ABSTRACT
Objective To explore the clinical effect of 3D printing in vitro modeling for minimally invasive treatment of complex pelvic fractures.Methods From April 2016 to June 2017, 18 cases of complex pelvic fractures were treated by 3D printing in vitro modeling were selected.The operation time and intraoperative bleeding volume were recorded.Fracture reduction was evaluated by X-ray, CT and Matta scores, and functional recovery was assessed by Majeed score.Results All 18 patients were followed up for 12 to 24 months, with an average of 15.8 months.The operation time of 18 patients was 80～160 minutes, with an average of (120±23.1) minutes;the amount of bleeding was75～160 m L, with an average of (113.6±29.1) m L;the length of incision was 7～14 cm, with an average of (9.3±1.8) cm.According to the Matta score, the fracture reduction was excellent in 12 cases, good in 4 cases, fair in 2 cases, the excellent and good rate was 88.9％.Majeed score was used to evaluate functional recovery 6 months after operation, the results showed that 13 cases were excellent, 4 cases were good and 1 case was fair, the excellent and good rate was 94.4％.No complications such as internal fixation loosening or breakage occurred during the follow-up period.Conclusion For complex pelvic fractures, 3D printing in vitro modeling assisted minimally invasive surgery can make the operation more precision with less trauma, reduce the incidence of complications, and speed up the recovery of patients.
ABSTRACT
Background: Taraxacum officinale G.H. Weber ex Wiggers is a wild plant used in folk medicine to treat several diseases owing to bioactive secondary metabolites present in its tissue. The accumulation of such molecules in plant cells can occur as a response against abiotic stress, but these metabolites are often deposited in low concentrations. For this reason, the use of a biotechnological approach to improve the yields of technologically interesting bioactive compounds such as anthocyanins is a compelling option. This work focuses on investigating the potential of in vitro T. officinale cultures as an anthocyanin source. Results: To demonstrate the suitability of anthocyanin induction and accumulation in calluses under specific conditions, anthocyanin was induced in the T. officinale callus. A specific medium of 5.5% sucrose supplemented with 6-benzylaminopurine /1-naphthaleneacetic acid in a 10:1 ratio was used to produce an anthocyanin yield of 1.23 mg g-1 fw. An in vitro dandelion callus line was established from this experiment. Five mathematical models were then used to objectively and predictably explain the growth of anthocyanin-induced calluses from T. officinale. Of these models, the Richards model offered the most suitable representation of anthocyanin callus growth in a solid medium and permitted the calculation of the corresponding kinetic parameters. Conclusions: The findings demonstrate the potential of an in vitro anthocyanin-induced callus line from T. officinale as an industrial anthocyanin source.
Subject(s)
Taraxacum/growth & development , Plant Development , Anthocyanins/metabolism , In Vitro Techniques , Kinetics , Plant Cells , PhytochemicalsABSTRACT
Adult-acquired flatfoot deformity (AAFD), which is mainly caused by posterior tibial tendon dysfunction (PTTD), is a common foot and ankle disease, and most of the deformities are flexible. How to explain the pathogenesis of AAFD and choose proper surgical treatment for the deformity has become a hot research focus nowadays. With the development of in vitro modeling technique for flatfoot, the accuracy and repeatability of the biomechanical tests have been gradually recognized, and the research results have also provided important theoretical basis for the clinical treatment of flatfoot deformity. In this article, the biomechanical mechanism of AAFD caused by PTTD, and the various modeling methods of flatfoot based on cadaver or finite element model were veviewed. The biomechanical characteristics of different reconstruction procedures in relative basic researches on flatfoot deformity were also analyzed and compared. The author believes that on the basis of simulating the dynamic stability of foot by tendon loading, the in vitro model of flexible flatfoot established by selective ligaments section is more reliable, and the reconstruction procedure adopted by various flatfoot models has different biomechanical characteristics. The soft tissue reconstruction and the bony procedures should be performed at the same time, and individual bony procedures should be chosen based on the degree and feature of the deformity.