ABSTRACT
Objective Our aim is to investigate the effects of lndomethacin on the expression of VEGF and the change of Micmves-sel Density(MVD)in remnant kidney of rats with 5/6 subtotal nephrectomy(STNx).Methods The renal subtotal ablation model was established by surgically 5/6 renal resection of the male Spragne-Dawley(SD)rats.Three groups were divided in our experimental protocol,including Sham,STNx and STNxI group.At the 8th week after gastric gavage,pathological changes of the remnant kidney were evaluated.Immunohistachemistry were used to examine the expression ofVEGF and MVD in the remnant kidney.Results At the 8th week after gastric gavage,only Up was significant decreased(P<0.05).TIS in Indomethacin group WaS significandy increased(P<0.05)and the preitu bular capillary density wa$significant decreased(P<0.05),compared with STNx group.Although BUN,Cr,the remnant kidney glomerulus's GSI were also decreased,the difference was not statistically significant(P>0.05).The glomendus～capillary density had no signifi-cant difference(P>0.05).In all rats,the expression of VEGF was positive correlated with capillary density between glomemlus and tubu-lointerstitimn(P<0.05).Conclusions Iadomethacin Can decrease UP in remnant kidney of rats with 5/6 subtotal nephrectemy(ST-Nx),and decrease the preitubular capillary density and aggravate mbulointerstitial injury at the same time.
ABSTRACT
The present experimental study was carried out to investigate the effect of indogenous prostaglandeins produced in the peripheral nerves after injury and the action of indomethacin ( a prostaglandin synthesis inhibitor) on their regeneration on the animal models with sciatic nerve crushed. The main results were: 1 , Radioimmunoassay demonstrated a stepladder-like increase in PGF 2 alpha in the sciatic nerve after crushing injury ; 2 , light microsocpy showed the number, diameter and area of the cross section of regenerated, myelinated nerve fibers passing through the crushed segment of the tibial fascicle of sciatic nerve of the rabbits being more than those injected with normal saline ; 3 , Culture of dissociated dorsal ganglia root cells from newborn rabbits revealed that nerve cells grown in media containing PGF 2 alpha showed significantly smaller size, less number of regenerated processes and lower content of RNA than those grown in media containing indomethacin. Scanning electronmicroscopy demomstrated different surface morphological features. These results indicated that an excess prostaglandin was produced in the rcushed nerve and inhibited nerve regeneration. Indomethacin as a prostaglandin synthesis inhibitor prevented excessive prostaglandin formation to promote nerve regeneration.