ABSTRACT
Objective To investigate the role of vestibular organs on stress analgesia induced by rotation in mice. Methods Female mice were randomly divided into morphine group and rotation group. After 15 minutes of intraperitoneal injection of naloxone or normal saline, the hot plate latency of mice in each group was observed following morphine injection or rotation (250 r/min, 15 s on with 5 s off). After subcutaneous injecting morphine for 7 consecutive days, tolerance was formed and the hot plate latency in morphine-tolerant mice after rotation was observed. P-aminophenylarsonic acid was injected into the inner ear to damage the vestibular organs of the mice and the hot plate latency was observed in chemically labyrinthectomy mice. Results Compared with the normal saline group, the hot plate latency of mice in the naloxone group did not change significantly after rotation (P>0.05), and the hot plate latency decreased significantly after subcutaneous injection of morphine (P<0.05). The morphine-tolerant mice had no significant change in the hot plate latency after rotation compared with the normal saline group (P>0.05). After injection of p-aminophenylarsonic acid into the inner ear, the recovery time of the righting reflex in mice was significantly increased, and the swimming ability was significantly reduced (P<0.05), and the hot plate latency of mice with chemical labyrinthectomy was significantly shortened after rotation (P<0.05). Conclusion Chemical labyrinthectomy completely blocked the rotation-induced stress analgesia in mice. Vestibular organs play an important role in rotation-induced stress analgesia, and this stress analgesia may be mediated by a non-opioid system.
ABSTRACT
Objective To investigate Iomoxicam,paracetamol,trarnadol in post-operative analgesia of surgical abortion.Methods 200 patients undergoing surgical abortion were randomly allocated into four groups:lornoxicam,paracetamol,tramadol and the control group.Intravenous anesthesia was conducted.Lomoxicam 16 mg,paracetamol 1000mg,tramadol 100 mg were administered before patients awaking.VAS scores and complications were observed post-operation.Pre-operative and post-operative salivary cortisol was detected.Results VAS scores were less in the 3 analgesic groups than in control group at 15,30 minutes post-operation(P＜0.05).VAS scores had no difference in all groups at 60,120 minutes.Cortisol was higher in the control on the next day(P＜0.05).Conclusion Post-operative analgesia was effective for surgical abortion.
ABSTRACT
The effects of cholecystokinin octapeptide (CCK-8) and its selective antagonists (proglumide, MK-329) on morphine induced analgesia (MIA) in the paw pressure test of the Wistar-SPF male rats were investigated. Baseline pain thresholds indicated that the morning (09:00-12:00) testing were significantly higher than the afternoon (14:00-18:00) testing. This result supports that diurnal variation of endogenous opioids. MIA was shown clear (p<0.01) dose-dependent (1.5-15mg/kg, i. p., n=6) manner with significant correlation coefficient (y=3.98x+22.67, r=0.827, p<0.001). CCK significantly (p<0.01) reduced MIA. Both proglumide (5-50mg/kg) and MK-329 (0.5-5.0mg/kg) were enhanced MIA. Especially, MK-329 has shown potently enhanced MIA. i. e. it showed desinhibition to the CCK's inhibitory action to MIA. These results suggest that CCK play important role to the endogenous opioids antagonist via through CCK-B receptors and to the function of the pain/analgesic mechanisms.