ABSTRACT
Objective@#To observe the effect of low concentration paraquat (PQ) on activation and phenotypic M1/M2 polarization of mouse microglia cells (BV2) .@*Methods@#BV2 cells were used as model, and cultured in vitro were exposed to paraquat at designed concentrations of 0, 0.015, 0.03, 0.06, 0.12, 0.24, 0.48 μmol/L and 0.05 μmol/L 1-methyl-4-phenylpyridinium (MPP+) for 24 h, and cell viability was determined by CCK8 assay. After induced by 0, 0.015, 0.03, 0.06, 0.12 μmol/L PQ and 0.05 μmol/L MPP+ for 24 h, the contents of tumor necrosis factor-α (TNF-α) , interleukin-6 (IL-6) and IL-1β in cell culture supernatant were determined by enzyme-linked inmunosorbent assay (ELISA) . Cell migration ability was determined by transwell. Immunofluorescence (IF) and flow cytometry were used to determine the phagocytic capacity of cells. Designed concentrations of 0, 0.03, 0.06, 0.12 μmol/L PQ and 0.05 μmol/L MPP+ for 24 h, the protein expressions of M1 markers of BV2 (TNF-α, IL-6, IL-1β, Nitric oxide synthase-iNOS, CD86) and M2 markers of BV2 (Arginase type-1 Arg-1 and Mannose recepteor-CD206) were determined by Western Blot after PQ expourse (0, 0.03, 0.06, 0.12 μmol/L) and 0.05 μmol/L MPP+ induction.@*Results@#Compared with 0 μmol/L PQ group, proliferation activity of BV2 cells was significantly increased by 0.03~0.12 μmol/L PQ while inhibited by 0.48 μmol/L PQ (P<0.05) . The cell proliferation activity of cells treated with 0.03 μmol/L PQ was significantly increased in 24 hours (P<0.05) . ELISA showed that TNF-α, IL-6 and IL-1β contents in the cell supernatant of the PQ group were significantly higher than those of 0 μmol/L PQ group, especially in 0.03 and 0.06 μmol/L PQ exposed group (P<0.05) . The results of IF and flow cytometry showed that phagocytic capacity of 0.015, 0.03 and 0.06 μmol/L PQ group was significantly enhanced compared with 0 μmol/L PQ group (P<0.05) . Transwell showed that the cell invasion ability of 0.03, 0.06, 0.12 μmol/L PQ was significantly higher than that of 0 μmol/L PQ group (P<0.05) . Western blot showed that compared with 0 μmol/L PQ group, the expression levels of M1 markers TNF-α, IL-6, IL-1β, iNOS and CD86 were significantly increased in 0.03 and 0.06 μmol/L PQ exposed group, while the expression levels of M2 markers Arg-1 and CD206 protein were decreased in 0.06 and 0.12 μmol/L PQ exposed group (P<0.05) .@*Conclusion@#Low concentration PQ can abnormally activate BV2 cell, making the transformation of BV2 cell into pro-inflammatory M1 type and inhibiting its transformation into anti-inflammatory M2 type.
ABSTRACT
Objective To observe the effect of Huoluojiangu Capsule(HJC) on anti-inflammatory experiment.Methods The anti-inflammatory experimental models were established in three kinds of rats with pleuritis,the swellen sole of the foot and the PGE_2 content in inflammatory tissues,respectively.Results The swollen reaction in the sole of foot in rats induced by egg white was lessoned with the treatment of low,middle and high dosage of HJC.Compared with the blank control group,the treatment is effective in low dosage group and evidently effective in groups of middle and high dosage after 30 min(P0.05),respectively.Compared with the blank control group,the effect of HJC on the PGE_2 content in the inflammatory tissues of middle and high dosage groups was significant(P