ABSTRACT
OBJECTIVE@#To elucidate the mechanisms of 4 effective components from a Chinese medicine formula, namely Qingre Huoxue Jiedu Formula (QHJ heat- and toxin-clearing and blood-activating formula), in the treatment of nerve growth factor (NGF)-induced psoriasis.@*METHODS@#Keratinocyte proliferation and T cell proliferation models were developed using NGF. An NGF solution (NGF+DMEM, 100 ng/mL) was added to all induced groups and treated groups and were cultured for 24 h, while a solution with NTRK1 antagonist (K252a+DEME, 300 nmol/L) was added and cultured for 1 h. The models were used to evaluate the effects of the treatment with each of the 4 components of QHJ, namely shikonin, paeonol, astilbin and ursolic acid. Cell apoptosis and proliferation were measured by flow cytometry analysis and CCK8 assay, respectively. The mRNA expression levels of Bax, Bcl-xl, and NGF receptor (NGFR) were assessed by quantitative real-time PCR (qRT-PCR) and Western blot analysis, respectively.@*RESULTS@#(1) All QHJ-treated groups showed significantly increased cell apoptosis and inhibition of cell proliferation compared with the NGF-induced groups (P<0.05). In addition, treatment with QHJ plus NTRK1 significantly enhanced cell apoptosis and inhibition of cell proliferation compared with cells treated with QHJ only (P<0.05), particularly in cells treated with ursolic acid. (2) QHJ-treated groups showed higher protein expression levels of Bax, Bcl-xl compared with other groups (P<0.05). Additionally, treatment with QHJ plus NTRK1 significantly increased the protein expression levels of Bax, Bcl-xl and NGFR compared with those treated with QHJ only (all P<0.05), especially in those treated with shikonin.@*CONCLUSION@#The action mechanism of QHJ on psoriasis might be through enhancing cell apoptosis and inhibition of cell proliferation, and upregulating the expression level of Bax, Bcl-xl and NGFR.
Subject(s)
Humans , Apoptosis , Drugs, Chinese Herbal/therapeutic use , Nerve Growth Factor/metabolism , Psoriasis/drug therapyABSTRACT
Se trata de una adolescente femenina de 16 años de edad, natural de Cuyagua estado Aragua y procedente de la localidad, quien presenta desde hace cuatro meses aproximadamente, prurito intenso a nivel del complejo areola pezón y exudado, amarillento, no fétido, de moderada cantidad en ambas mamas. Refiere haber consultado a facultativo quien indica tratamiento, a base de oxacilina no presentando mejoría. Acude el día 05/04/16 al Centro Integral de Especialidades "Los Grillitos", CORPOSALUD. Municipio Mario Briceño Iragorry a consulta especializada. Al examen físico se evidencia: mamas simétricas, complejo areola pezón eritematoso, con zonas de descamación, exudado amarillento y a nivel de mama izquierda se extiende hasta piel del cuadrante ínfero interno; se indica realizar paraclínicos: ecosonograma mamario, hematología completa, VSG, PCR y se toma muestra para cultivo, de la misma manera se indica tratamiento con corticoides tópicos y antibióticoterapia (ciprofloxacina). Acude a control el 15/04/16 con evolución clínica satisfactoria y resultado de cultivo positivo para S. aureus, sensible a ciprofloxacina.
It is female 16-year-old native of Cuyagua state Aragua and from the town, who presents from four months about intense itching at the level of the nipple areola complex and not fetid yellowish exudate moderate amount of both breasts, which refers consultation with physician who indicates treatment based oxacillin presented no improvement. She goes on 5/4/16 at Integral Center specialty "The Grillitos" CORPOSALUD. Municipio Mario Briceño Iragorry a specialist consultation, physical examination is evident: symmetrical breasts, nipple areola complex erythematosus, with areas of desquamation and yellowish exudate, and level of left breast extends to skin internal bottom quadrant shown performing paraclinical: sonogram breast, complete blood count, ESR, CRP and sample is taken for cultivation, in the same way treatment with topical corticosteroids and antibiotics (ciprofloxacin) is indicated. Control goes to 04/15/16 with satisfactory clinical evolution resulting in positive culture for S. aureus sensitive to ciprofloxacin.