Follow-up of a Composite Endomycorrhizal Inoculum in the Rhizosphere of Olive Plants, Analysis after 42 Months of Culture

Endomycorrhizal inoculum, consisting of more than 26 mycorrhizal species, has been followed over time at the rhizosphere of olive trees. The evolution during time of this endomycorrhizal inoculum at the rhizosphere of plants has been discussed in this study. After 42 months, 45 species were isolated from the rhizosphere of inoculated olive plants. These species belong to 6 genera (Glomus, Acaulospora, Gigaspora; Scutellospora; Pacispora and Entrophospora), from these genera, Glomus was the most dominant (40%) followed by the Acaulospora (30%). Glomus constrictum and G. intraradices were the most abundant species, their frequency are respectively 17% and 15%. In comparison between detected species, those of primary inoculum and those recovered after 30 months, 36 endomycorrhizal species appeared and 14 species disappeared, but four species G. clarum, G. intraradices, G. mossea and G. versiforme have been able to maintain their status and stability of multiplication in the rhizosphere of olive plants.

Mycorrhizal Cultivation of Dendrobium officinale / 中国药学杂志

OBJECTIVE: To provide the scientific basis for the application of mycorrhizal fungi in Dendrobium officinale artificial cultivation. METHODS: The dominant strain was screened in a planting base by using biomass as the index. The effects of initial inoculums and supplementary inoculation on the growth and the chemical composition of D. officinale were further investigated. RESULTS: Mycorrhizal fungi M2(Mycena sp.) was found to be the advantageous strain. After 16 months of growth, the stem dry weight(SDW) and plant dry weight(PDW) of the three M2-treatment groups were significantly higher than those of CK, and the SDW of group M(M2 initial inoculums of 1.0 g·clump-1) was the highest, which increased by 39.2% and 11.1% compared with CK and group L(M2 initial inoculums of 1.0 g·clump-1)(P<0.05), respectively. Furthermore, M2 had no effect on the chemical properties of stem polysaccharide, and could improve the contents of naringenin and bibenzyls in the stems. The supplementary inoculation of M2 enhanced the plant-growth-promoting effects of M2. The SDW of the supplementary group increased by 18.0%(P<0.05) compared with the non-supplementary group, while supplementary inoculation had a certain effect on the chemical properties of polysaccharide. CONCLUSION: Mycorrhizal fungus M2 has good application prospects for D. officinale artificial cultivation.

Inoculação de Sclerotinia sclerotiorum em sementes de oleaginosas: transmissão e seus efeitos sobre a emergência de plantas / Inoculation of Sclerotinia sclerotiorum in seed of oleaginous plants: transmission and effects on emergence

O trabalho propôs-se a verificar a transmissibilidade de Sclerotinia sclerotiorum de sementes de canola, cártamo, crambe, girassol, nabo forrageiro e níger inoculadas artificialmente e suas implicações na emergência de plântulas. O isolado fúngico foi repicado em placas de Petri, contendo meio BDA, incubado a 20ºC e fotoperíodo de 12 horas. Após o crescimento do patógeno, foram colocadas 50 sementes de cada cultura por placa, onde permaneceram por 20 horas. Como testemunha, utilizaram-se sementes incubadas nas mesmas condições, porém apenas em meio BDA. Observou-se que S. sclerotiorum pode ser transmitido para as plântulas das culturas quando associado às suas sementes, sendo estas uma importante fonte de inóculo. O fungo provocou tombamento de pré e pós-emergência em todas as espécies estudadas.

The study aimed to verify the transmission of Sclerotinia sclerotiorum of rapeseed, safflower, crambe, sunflower, radish and niger seed (artificially inoculated) and its implications on seedling emergence. The fungal isolate was peaked in Petri dishes containing PDA medium and incubated at 20°C and 12 hours photoperiod. After the growth of the pathogen, 50 seeds were placed on each plate cultures where they remained for 20 hours. As a control we used seeds incubated under the same conditions, but only on PDA. It was observed that S. sclerotiorum can be transmitted to seedlings of crops when associated with its seeds, being an important source of inoculum. The fungus caused damping-off in all of the studied species.

Avaliação dos modelos logístico bicompartimental e de Gompertz na estimativa da dinâmica de fermentação ruminal in vitro do farelo e da torta de babaçu (Orbignya martiana) / Avaliation of bicompartimental logistic and Gompertz mathematical models to estimate gas production from babassu (Orbignya martiana) meal and pie using the semi-automated in vitro technique

Utilizaram-se dois modelos matemáticos para avaliar a produção de gases do farelo e da torta de babaçu, pela técnica in vitro semiautomática de produção de gases. Foram utilizados o modelo logístico e o de Gompertz. Os parâmetros de validação usados foram o quadrado médio do erro (QME), o coeficiente de determinação (R²), o desvio médio absoluto dos resíduos (DMA) e a análise gráfica dos resíduos. O modelo logístico bicompartimental apresentou menores valores (P<0,05) para o QME e o DMA em relação ao de Gompertz, e não houve diferença (P>0,05) quanto ao R². Os gráficos de dispersão mostraram semelhanças nos ajustes dos dois modelos. Na análise gráfica dos resíduos, os dois modelos descreveram bem cinética de produção de gases da matéria seca. No entanto, o modelo logístico apresentou melhor valor de QME. Para avaliação da cinética de fermentação ruminal do farelo e da torta de babaçu pela técnica in vitro semiautomática de produção de gases, recomenda-se adotar o modelo logístico.

Two mathematical models were used to evaluate gas production from the meal and pie of babassu using the semi-automated gas production technique. The logistic and Gompertz models were used and the validation parameters for both models were the residual mean square (RMS), the coefficient of determination (R 2 ), the absolute average residual (AAR), and the graphical analysis of residues. The logistic model showed lower RMS (P<0.05) and AAR in comparison to Gompertz model. Both Gompertz and logistic models showed similar R 2 . The dispersion graphics showed similarity for both models and graphics analyses demonstrated that both models describe well the kinetic of gas production of dry matter. However, the bicompartimental logistic model showed the best RMS value. Logistic models are recommended to describe the kinetic of gas production from babassu foods using the semi-automated in vitro technique.

DINÁMICA MICROBIANA EN VERMICOMPOSTAS COMERCIALES CON Y SIN INOCULACIÓN DEL HONGO Trichoderma spp / Microbial dynmiccs in commercial vermicomposts with and withot trichoderma sspp. fungus inoculation

En el presente trabajo se presentan los resultados de un ensayo en el cual se evaluó la efectividad de la inoculación del hongo Trichoderma spp, en una vermicomposta comercial, comparándose ésta con la población del hongo presente en un vermicompost sin inocular. El experimento se realizó en el laboratorio de postcosecha de la Universidad de Costa Rica. Se desarrolló en tres fases (cultivo, aislamiento e inoculación de cepas del hongo) y se utilizaron tres vermicompostas comerciales elaboradas a partir de un sustrato de broza de café, procedentes de diferentes composteras. Los resultados muestran que no hubo aumento ni disminución de la población de Trichoderma como producto de la inoculación de cepas del mismo.

In tThis paper, presents the results are presented onof the growthefectiveness of the Trichoderma fungus in a inoculation in a commercial vermicompost, in comparison with the fungus from a vermicompostand without inoculation. In a three-phase laboratory process (cultivation, isolation and inoculation of fungal strains), three different commercial vermicompost, prepared from coffee pulp, have beenwere used, coming from three different commercial vermicomposts. The results of experiments carried out in the Post-Harvest Laboratory of the Universidad de Costa Rica, don’t show any increase or decrease of the Trichoderma population after inoculation. The experiment was carried out in the Post -harvest Laboratory of University of Costa Rica. It was developed following three phases, using three commercial vermicompost prepared with broza of coffee. Results showed no differences in Trichoderma population as product of inoculation of it into the compost.