Phenotype identification of CD8αα+ T cells infiltrating psoriatic skin lesions / 中华皮肤科杂志

Objective To identify the phenotype of CD8αα+ T cells locally infiltrating psoriatic skin lesions,and to investigate their role in the occurrence of psoriasis.Methods Skin lesions were obtained from 8 patients with confirmed plaque psoriasis in the progressive stage,who visited the Department of Dermatology in Xijing Hospital affiliated to the Fourth Military Medical University from January to December in 2017.Of the 8 patients,4 were male and 4 were female,with ages ranging from 24 to 50 years.Normal skin tissues were obtained from discarded skin tissues of 8 healthy controls in plastic surgery.Of the 8 healthy controls,4 were male and 4 were female,with ages ranged from 23 to 46 years.Immunofluorescence technique was used to investigate the distribution of CD8αα+ T cells,determine the proportion of CD8αα+ T cell subsets,identify the immunological phenotype of CD8αα+ T cells and measure the expression of interleukin-17A (IL-17A).Results Infiltration of CD8 + T cells was observed in the dermis and epidermis of the 8 psoriatic skin lesions,and the proportion of CD8αα+ T cells was 88.48％ ± 7.39％.However,only a few CD8+ T cells locally infiltrated the control skin tissues,and the proportion of CD8αα+ T cells was 14.43％ ± 13.14％.There was a significant difference in the proportion of CD8αα+ T cells between the psoriatic skin lesions and control skin tissues (t =11.5,P ＜ 0.01).CD8αα+ T cells in the psoriatic epidermis expressed CD103 (a marker of tissue-resident cells),while CD8αα+ T cells in the psoriatic dermis did not express CD103.CD8αα+ T cells in the psoriatic lesions were identified as CD45RA CCR7 effector T cells,and did not express Foxp3,CD25 and CD122 (markers of CD8+ regulatory T cells).The proportion of CD8αα+ T cells producing IL-17A in the psoriatic lesions was 24.85％ ± 4.25％,while CD8αα+ T cells in the control skin tissues did not produce IL-17A.There was a significant difference in the proportion of CDSαα+ T cells producing IL-17A between the psoriatic skin lesions and control skin tissues (t =5.853,P ＜ 0.01).Conclusion CD8αα+ T cells infiltrating psoriatic lesions are effector memory T cells,and may contribute to the occurrence and development of psoriasis by producing IL-17A.

Expression of midkine and interleukin-17 in rheumatoid arthritis patients and its clinical significance / 中华风湿病学杂志

ObjectiveTo study the midkine(MK) and interleukin(IL)-17 levels in the sera of rheumatoid arthritis(RA) patients. MethodsMK, IL-17 and anti-CCP levels in the sera were detected by ELISA in 79 patients with rheumatoid arthritis, 70 healthy controls and 37 patients with osteoarthritis (OA).Krusk-Wallis H test, Mann-whitney U test and Spearman's correlation were used for statistical analysis.ResultsThe serum level of MK of RA patients(327±167) pg/ml was significantly higher than healthy controls (225±109) pg/ml and OA patients (209±130) pg/ml (H=22.01, P＜0.01), but there was no significant difference between that of the healthy controls and OA group (P＞0.05). The serum level of IL-17 (44±15) pg/ml from RA patients was increased significantly than healthy controls (27±8) pg/ml and OA patients (26±8) pg/ml(H=66.89, P＜0.01 ), but there was no significant difference between healthy controls and OA group(P＞0.05). Serum MK values were correlated with IL-17 and CRP(r=0.398, P＜0.01; r=0.285, P＜0.01 ), but not with DAS, RF, ESR, AKA and anti-CCP. ConclusionThe serum level of MK and IL-17 from RA patients is increased significantly than healthy controls and OA patients, so MK and IL-17 may be involved in the pathogenesis of RA.