ABSTRACT
Objective To investigate the expression and prognostic significance of CD151, c-Met and integrin alpha 3, alpha6 in pancreatic ductal adenocarcinoma (PDAC). Methods The expression of CD151, c-Met and integrin alpha3, alpha6 in 71 patients with PDAC and 10 samples of normal pancreas tissues were detected by immunohistochemistry, and the relationship between the expression of CD151, c-Met and integrin alpha 3, alpha 6 and the clinicopathological features, prognosis of these patients was analyzed. Results The positive expression rates of CD151, c-Met and integrin alpha 3, alpha 6 in PDAC were 81.69% (58/71) , 69.01% (49/71), 69.01% (49/71) and 84.51% (60/71) , and there was no expression in normal pancreas tissues. The expressions of CD151, c-Met were significantly associated with TNM stage and lymph node metastasis (P < 0.05). The expression of CD151 was positively correlated with the expressions of c-Met and integrin alpha3, alpha6 (r =0.583, P =0.000, r = 0.457;P =0.000, r = 0.671 ;P =0.000). Univariate analysis suggested the expression of CD151, c-Met and integrin alpha3, alpha6 was associated with survival (P<0.05). Multivariate analysis suggested the expression of CD151, c-Met was the independent prognostic factor for post-operative survival. Conclusions CD151, c-Met and integrin alpha3, alpha6 play a role in the development, metastasis and prognosis of PDAC, and they might be new markers to predict biological behavior and the prognosis of PDAC patients.
ABSTRACT
ObjectiveTo study the effect of astragaloside Ⅳ(AS-Ⅳ) on glucose-induced podocyte adhesion and its possible mechanism. MethodsConditionally immortalized mouse podoeytes were treated with 10, 50, 100 mg/L AS-Ⅳ and with 100 mg/L AS-Ⅳ for 3, 6, 12, 24 h. Cell attachment was measured by fluorescence and centrifugation cell adhesion assays, respectively. Expression of α3β1 integrin mRNA and protein was examined by real-time PCR and Western blot. ResultsHigh glucose induced a significant reduction in adherent podocytes compared to normal glucose group (P<0.05). AS-Ⅳ improved high glucose-induced podocyte adhesion in a time- and dose-dependent manner. Real-time PCR and Western blot analysis revealed that high glucose-induced down-regulation of α3β1 integrin in podocytes were significantly meliorated by AS-Ⅳ (P<0.05). ConclusionAstragaloside Ⅳ improved high glucose-induced podocyte adhesion which may be mediated through α3β1 integrin up-regulation.
ABSTRACT
Objective: To detect the expressions of integrin alpha 3 and matrix metalloproteinase-9 (MMP-9) in pancreatic adenocarcinoma, and investigate their relationahip with clinicopathological parameters, furthermore analyze the correlation between integrin alpha 3 and MMP-9. Methods: We collected 100 cases of paraffin-embedded pancreatic adenocarcinoma tissues. The expressions of integrin alpha3 and MMP-9 were detected by immunohistochemical labelled streptavidin biotin ( LsAB) methods. Results: Ninety eight percent pancreatic adenocarcinomas were integrin alpha3-positive and 75% pancreatic adenocarcinomas highly expressed integrin alpha 3. The expressions of integrin alpha 3 in head, neck and uncinate pancreatic adenocarcinomas were stronger than those in pancreatic adenocarcinomas located at body and tail (P=0.041). Increased expression of integrin alpha3 was associated with local lymph node metastasis (P=0.012). Eighty one percent pancreatic adenocarcinomas expressed MMP-9 and 39% of them showed strongly positive. There was no correlation between the expression of MMP-9 and age, gender, tumor location, tumor grade, duodenum invasion, local lymph node metastasis, perineural invasion or TNM stage. The expression of MMP-9 positively correlated with the expression of integrin alpha 3. Conclusion: Expression of integrin alpha 3 correlates with the location of pancreatic adenocarcinomas and plays a role in local lymph node metastasis. Integrin alpha 3 might up-regulate the expression of MMP-9.