ABSTRACT
Objective To study the intervention effect and mechanism of integripetal rhodiola herb (IRH) on Alzheimer’s disease model rats. Methods Alzheimer’s disease model was made by injecting D-galactose (10 mg/mL) in abdominal cavity and taking AlCl3 (20 mg/mL) by intragastric administration, and injecting scopolamine. Morris water maze testing system was undertaken to observe the change of learning and memory ability in rats, biochemical indicator tested was to contrast the state of oxidative stress, common HE staining and immunohistchemistry staining were to observe the morphology change of the hippcampus and cortex cell. The model rats were administrated IRH by means of intragastric administration contrasting with hydergine to compare the effect. Results The memory ability of model group was obviously decreased, cellula nervosa were damaged badly. A? and senile plaques appeared on hippcampus and cortex. All of the test indexes in Morris water maze showed that the abilities of space search, learning and memory in different groups remarkably improved compared with that of the model group (P
ABSTRACT
Objective:To investigate the effect of IRH on pulmonary fibrosis and its intervention mechanism.Methods:45 sprague-dawley rats were randomly divided into three groups.The model group and the intervention group were received endotracheal injection of bleomycln to induce pulmonary fibrosis,while the control group were received endotracheal injection of saline.From the second day the intervention group IRH were injected to the stomach,while the other two groups were injected saline instead.On the 7th day,14th day and 28th day the lungs were isolated.The expression of MMP-2 and TIMP-1 was detected by immunohistochemical method respectively.Results:There was only a very weak expression of MMP-2 and TIMP-1 in the control group,there was significantly higher level in model group compared with control group on the 7th day,14th day and 28th day.The IRH group was significantly lower than the model group with the same time points,though it was also obviously higher than the control group all over the period(P