ABSTRACT
Interferon regulatory factor 7 is originally identified in the context of Epstein-Barr virus infection,and has play a key role in regulator of type Ⅰ interferons.Aberrant production of type Ⅰ interferons is associated with many types of diseases such as cancers and autoimmune disorders.Furthermore,mounting evidence has shed light on the importance of interferon regulatory factor 7,which is a multifuwtional transcription factor,not only regulate the response of inflammation and immune in body,but also regulate others biological funetions such as tumor formation,cell invasion and so on.The article has reviewed the progression of both interferon regulatory factor 7 and tumor.
ABSTRACT
Interferon regulatory factor 7 (IRF7) is one of the transcriptional factors for the activation of type I Interferon (IFN) genes. It is known that IRF7 and the latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) are highly expressed in EBV type III latency cells, and LMP1 induces mRNA expression of IRF7. In this study, the expression pattern of endogenous IRF7 was observed in several B cell lines with or without EBV infection by immunofluorescence staining. IRF7 was localized in the cytoplasm of EBV-negative B cells and EBV type I latency B cell lines. However, IRF7 was located both in the cytoplasm and nucleus of EBV type III latency cell lines. In the Jijoye cell (type III latency cell), IRF7 was colocalized with LMP1 in the cytoplasm in a capping configuration, and their interaction was confirmed by co-immunoprecipitation of LMP1 and IRF7. This colocalization was confirmed by co-transfection of IRF7 and LMP1 plasmids in EBV-negative B cells. These results suggest that the IRF7 and LMP1 interact with each other, and this may relate to the mechanism whereby LMP1 exerts functional effects in B-lymphocytes.