ABSTRACT
AIM: To investigate the dynamic influence of zanthoxylum seed oilA2(ZSOA2) on NF-?B signaling pathway and inflammatory factor in pulmonary tissue of asthmatic mice.METHODS: The suspensoid(0.2 mL containing 20% albumin hydroxide and 10% ovalbumin) was administered by intraperitoneal injection to sensitize the BALB/c mice on day 1,then 0.4% ovalbumin solution(50 ?L in phosphate buffer fluid) was dripped into the respiratory tract through nasal cavity to establish the asthmatic mouse model.After dripped ovalbumin for 24 h,48 h,3 d,7 d and 14 d,the mice were killed at specified time points.The contents of interleukin-4(IL-4),interleukin-5(IL-5) and interferon-?(IFN-?) in bronchoalveolar lavage fluid(BALF) were determined by ELISA.The pathological changes of the lung tissues were observed with HE staining.The inflammatory cell counts were conducted by Eosin staining.The protein levels of adhesion molecule and the molecules of NF-?B signaling pathway in lung tissues were determined by Western blotting.RESULTS: In ZSOA2 treated mice,the pathological injury of the lung was significantly attenuated as compared to the model mice,the counts of eosinophils and lymphocytes were reduced obviously in lung bronchial area of asthmatic mice at all observed time points(P
ABSTRACT
AIM: The expression of the Fas Antigen and induction of apoptosis by anti-Fas antibody in esoinophils in vitro were investigated. METHODS: Purified eosinophils from health donors were cultured for 72 h in the presence of human IL-5 and with or without anti-Fas monclonal antibody (MoAb) at various concentrations (1-1000 ?g/L). The expression of the Fas antigen on eosinophils was determined by immunocytochemistry. The changes of eosinophils viability and apoptosis were also studied. RESULTS: The Fas antigen was expressed on freshly isolated eosinophils, which had no significant changes after culture in the presence or absence of IL-5. The anti-Fas MoAb at different concentration suppressed significantly the IL-5-mediated eosinophils survival (78%?9%). When eosinophils were cultured in the presence of IL-5 (1?10 4 U/L) with anti-Fas MoAb (1 000 ?g/L), the percentage of alive cell decreased to 30%?12%( P