ABSTRACT
Objective:To investigate the changes of somatostatin ,epidermal growth factor receptor ,prostaglandin E2 and mucus of ileum in SD rats injected aspirin.Methods:32 SD rats were randomly divided into 4 groups:oral intake group ,coloclyster group ,in-traperitoneal injection group and normal control group.Ileal tissue sections were stained by Alcian blue.The positive area and IOD of Alcian blue was counted by image analysis in each group.The concentration of SST ,EGFR and PGE2 were detected by ELISA immuno-histochemistry ,and the IOD of each index was measured by image analysis in each group.Results:The score of ileum mucosal injury were highest in aspirin treatment groups ( P<0.05 ) ,but the score was no difference among aspirin treatment groups.The area and IOD of mucus in aspirin treatment groups were less than normal control group .The Area ( 602.17 ±158.03 ) and level of IOD ( 249.54 ± 113.19) in intraperitoneal injection group was the lowest (P<0.05).There were no differences of level of SST ,EGFR was observed among all groups .The concentration and IOD of PGE2 in normal control group was the highest (P<0.05).However,his discrepancy was not obvious among three groups of aspirin.Conclusion: Aspirin, in the four routes of administration , can cause damage to the mucosa of the rat small intestine in two weeks.The integrity of the small intestinal mucosa and mucus secretion were damaged significantly ,and it was the worst in intraperitoneal injection group.These changes might be correlated with the reduction of PGE 2.No clear results showed that the SST and EGFR were involved in the pathophysiology of the NSAIDs .
ABSTRACT
Objective To evaluate the effect of enteral nutrition emulsion on the immunologic function and intestinal mucous barrier in diabetic patients. Methods Eighty diabetic patients were randomly divided into con-trol group (n=40) and enteral nutrition group (n=40). The urine lactulose (L) and mannitol (M) levels and the blood immunologic indicators recorded on day 1 and day 8. Results The L/M ratio was significantly lower in enteral nutrition group than in control group on day 1 and day 8 ( P < 0. 05 ). The IgG level was significantly higher in enteral nutrition group than in control group on day 8 ( P = 0. 02 ). Conclusion Enteral nutrition emulsion can decrease the permeability of intestinal mucous membrane and improve the immunologic function in diabetic pa-tients.
ABSTRACT
Objective:To investigate the protective effect of the ischemic preconditioning on intestinal mucosal barrier after the rats' pancreas transplantation and analyze the possible mechanism.Methods:Group control included 12 normal rats received sham operation,36 steptozozin-induced diabetic SD rats were randomly assigned to 3 groups: Group I/R consisted of 12 diabetic rats which received pancreas transplantation alone,Group DIPC consisted of 12 diabetic rats which received pancreas transplantation with ischemic reperfusion in the pancreas graft before ablating donors,Group DIPC consisted of 12 diabetic rats which received pancreas transplantation with ischemic reperfusion in the recipient.The intestinal permeability,absorptional function and morphology,the TNF-?,NO,SOD and amylase activity in serum,MDA,SOD and MPO in intsetinal tissue were monitored 5 day after reperfusion.Simultaneitily,the bacteria translocational ratio of mesentery lymph node,liver and spleen were observed.Results:The TNF-? level,the amylase activity in serum(P0.05),the degree of intestinal mucous injury,the apoptosis index(P