ABSTRACT
@#ObjectiveTo investigate the immune tolerance function and significance of allogene bone marrow injection to the small intestines transplantation of rats.MethodsInbreeding line rat F344/N and Wistar/A were selected to perform heterotopic graft of the whole small intestine. 7 days before allogene transplantation, donator bone marrow cells (BMC) were injected into thymus of acceptor (the testing group). According to the isogene and allogene rat transplant model, it was comprehended whether injecting allogene donator marrow into acceptor thymus could decrease the acute rejection after transplantation.Results3, 5 or 7 days after allogeneic rat dystopia whole small intestine transplantation, typical reject reaction appeared, but there was no reject reaction in isogenome and testing group. 3 days after allotransplantation, serum soluble interleukin-2 receptor (sIL-2R) and tumor necrotic factor-α (TNF-α) levels were significantly higher than the other groups (P<0.01). The level of serum sIL-2R and TNF-α in the allogene marrow injecting group were only slight higher on the 3rd or 5th day, and getting downtrend, and there was no significant difference compared with isogenic transplantation group.ConclusionAllogenic donator bone marrow intrathymic injecting into acceptor 7 days before small intestina transplantation, can reduce the reject reaction after the grafting. The levels of serum sIL-2R and TNF-α can be selected as a sensitive early diagnosis index of acute rejection after small intestine transplantation.
ABSTRACT
Objective:To investigate the effect of intrathymic injection of allogenic antigen to sciatic nerve transplantation.Methods:C57BL/6(H-2b) mice were used as donors and BALB/c(H-2d) as recipients. The recipients were divided into four groups: auto-transplantation group, allogenic transplantation group, allogenic transplantation and using immunosuppressive drugs,intrathymic injection group: 3 mol/L KCl MHC antigen extractions were injected into the recipients’ thymus before two weeks before the sciatic nerves were transplanted. In the third week all the recipients underwent immunological detections for IL-2R,TNF-?,mixed lymphocytes culture and apoptosis.Results:All the detections indicated that it was of significant difference between intrathymic injection group and those in allogenic transplantation group.Conclusion:The immunological rejection of allogenic peripheral nerve transplantation can be somewhat inhibited by intrathymic injection of allogenic MHC antigen.
ABSTRACT
Objective:To study the effects of intrathymical injection allogenic antigen on the establishment of donor specific transplantation immunotolerance.Methods:H 2 antigen extracted from splenic cells of donor C56BL/6 mice was intrathymically injected to recipients adult Balb/c mice, one week later,donor C57BL/6 or C3H skingrafts were transplanted,observing transplanted skin survival times ,meanwhile MLC?CML ?DTH was detected.Results:the median survival time (MST) of transplanted skin allografts was over 70 days in tolerance recipients while the skin MST graft in the control group was 12.6?1.69 days,tolerance recipients normally rejected the donor C3H skin grafts (MST 13.4 ? 1.42 days), MLC detection showed that tolerance recipient splenocyte responded to C57BL/6 stimulator significant reduction ,but in normal reaction to donor C3H stimulator,the CML and DTH of tolerance recipient splenocyte to target cell of donor C57BL/6 was inhabited.The results suggested that slpecific tolerance might be induced by intrathymic injection. Conclusion:The intrathymic injection of donor H 2 antigen extracted from C57BL/6 splenic cells could induce recipients specific tolerance to skin transplantation.