ABSTRACT
Objective: To study the chemical constituents from the roots and rhizomes of Valeriana jatamansi in Valeriana Linn., Valerianaceae. Methods: The chemical constituents were separated and purified by silica gel, medium pressure column chromatography, and preparative HPLC. Their structures were determined by 1D, 2D NMR, HRMS, and IR spectroscopic analyse. Results: An iridoid ester was isolated from the dichloromethane extract from the roots and rhizomes of V. jatamansi, which was named as valerjatadoid C. Conclusion: Compound 1 is a new iridoid ester.
ABSTRACT
Objective: To purify the iridoid ester fraction of ethyl acetate extract from Patriniae Radix (PHEBB), and to study the antitumor effect and mechanism in vivo and in vitro. Methods: The silica gel column chromatography was used to purify the effective fraction. The chemical constituents of PHEBB were elucidated by RP-HPLC; MTT assay was used to study the inhibition of PHEBB on human oral epithelial cancer cells KB, human colon cancer cells COLO-255, and human gastric cancer cells SGC-7901; Mice transplanted S180 and H22 experiments were used to study the antitumor effect in vivo; The immunohistochemical method was used to study the effect of IEEAM on the expression of related genes in tumor tissue. Results: The main effective components in PHEBB were desacetylisovaltratum and isovaltrate acetoxyhydrin, and they are both iridoid esters. The results of MTT showed that PHEBB had the significant inhibition on KB, COLO-205, and SGC-7901 cells, and the IC50 values were within 2.27-5.49 μg/mL; The experiment of mice transplanted tumor showed that after ip injection with PHEBB at a dose of 60 mg/kg, there was an inhibitory rate of 45.5% on S180 and 54.2% on H22; The immunohistochemistry results showed that PHEBB could upregulate Bax expression in H22 cells, decrease Bcl-2 expression, and reduce the microvascular density in tumor tissue. Conclusion: The main components in PHEBB are iridoid esters, and have certain antitumor effect in vitro and in vivo; the antitumor mechanism of PHEBB might be associated with the induction of apoptosis and the inhibition of tumor microvessel.