ABSTRACT
This study was aimed to establish the quality and quantity analysis methods of tectoridin contented in the Iris tectorum Maxim. Thin layer chromatography (TLC) method was used to give quality analysis on tectoridin in I. tectorum. And the HPLC method was used to give quantity analysis on tectoridin in I. tectorum. The Diamonsil C18 (200 mm í 4.6 mm, 5 μm) column was used as analytical column. The acetonitrile-0.2% phosphoric acid (20:80) was used as mobile phase at the flow rate of 1.0 mL·min-1. The detection wavelength was 265 nm and the column temperature was 25℃. The results showed that the quality analysis of I. Tectorum had specific identification with-out the interference of other ingredients. The amount of inlet tectoridin had a good linearity with the response val-ue of peak area in the range of 0.12~2.22 μg. The average recovery rate was 100.96%. It was concluded that this method was simple and reproducible, which can be used in the quality control of I. tectorum.
ABSTRACT
Mangiferin, one of the active constituents of Rhizoma Belamcandae, in samples of Be-lamcanda chinensis (L.) DC. or its substitute was determined quantitatively by RP-HPLC. The 11 sam-ples collected from different localities for analysis were: 7 rhizomes of wildly grown or cultivated B. chi-nensis, 1 of its leaf and stem, and 3 substitutes (a wildly grown and another commercially available Iristectorum Maxim. and a I. dichotoma Pall. ). Results of the analysis showed that the contents of mangiferinin Rhizoma Belamcandae were significantly higher than that of its substitutes I. tectorum and I. di-chotoma. There were also certain significant differences between samples from different localities (P<0.05), but with no statistically significant difference between the rhizome or leaf and stem, neither be-tween cultivated and wildly grown samples, (P>0.05). The method was proved to be quick, simple andreproducible, and may provide a reliable basis for the quality control and evaluation of B. chinensis.
ABSTRACT
Object To establish an analysis method for determ in ation of irone in Iris tectorum Maxim. f. alba Makino. Methods The three isomers of irone were quantitatively analyz ed by GC-MS. Irone in I. tectorum extract was determinated by GC. Results The three isomers of irone, ?, ?, ?- irone were s p eculated according to the MS splitting decomposition law. Irone contents in the extract were 687, 238 ?g/g (n=6), which h ad much difference. Conclusion The analysis method for irone by GC-MS and GC is hig her efficiency, precise, and the analysis time is acceptable.