ABSTRACT
OBJECTIVE: To extract and isolate Isatidis indigotica polysaccharide A(with uniform molecular weight) and to study its structure.METHODS: Hydrosoluble crude polysaccharides were extracted from Isatidis indigotica before being separated and purified by column chromatography to obtain polysaccharide A.The purity and relative molecular weight of polysaccharide A were determined by high performance gel chromatography(HPGPC) and the chemical structure each polysaccharide constituent was studied by UV detection,IR spectral analysis,NMR detection,periodate oxidation and Smith degradation methods.RESULTS: The average molecular weight of polysaccharide A was 11 700.Arabinose and galactose were the major monosaccharide;? was the major configuration of glycosidic bond was predominantly,and 1→3 was the main linkage while 1→4 and/or 1→2 linkages were also presented in polysaccharides A.CONCLUSION:Isatidis indigotica polysaccharides A is a new compound,and its purity remains as high as above 96%.
ABSTRACT
Anthranilic acid in the leaves of Isatidis indigotica Fort. was determined by first orderderivative UV spectrophotometry. The average recovery was 96.66％ and the coefficient of deviation was1.09％. The method showed no significant difference as compared with high performance capillary elec-trophoresis at 95％ confidence level. The method is simple, rapid and accurate.