ABSTRACT
OBJECTIVE: To screen the habitat processing technology of Isatis tinctoria slices, and provide basis for its quality.METHODS: Appearance score, the contents of moisture, ash, extract and main component (R, S) -goitrin in I. tinctoria slices prepared by 4 kinds of dried degree [A. 60 ℃ drying after cutting; B. cutting after natural drying; C. cutting after natural drying (13-15 d), drying at 60 ℃ again; D. cutting after natural drying (30-50 d), drying at 60 ℃ again]from fresh harvested sample. RESULTS: Appearance score was in descending order with C>D>A>B; the content of moisture was in descending order with B>A>D>C. There was little difference in the content of ash among different methods. The contents of extract and (R, S)-goitrin were in descending order with C>A>B>D. CONCLUSIONS: The quality of I. tinctoria slices prepared by 4 processing technologies are different, and the quality of I. tinctoria slices prepared by cutting after natural drying (about 15 d)and drying at 60 ℃ again is relatively best.
ABSTRACT
To detect possible pathogenic virus(es) in woad (Isatis tinctoria) cultivated at Institute of Medicinal Plant Development in Beijing, reverse transcription(RT)-PCR was performed using total RNA of symptomatic woad leaves with primers for poty-, polero-, tobamovirus, broad bean wilt virus 2(BBWV2) and cucumber mosaic virus (CMV). A 657 bp fragment was amplified from symptomatic woad using CMV primers. Sequencing and BLAST analysis indicated that this fragment shared 99% nucleotide identity and 100% amino acid identity with CMV-Vi isolate. The isolate was named CMV-Isatis tinctorial (CMV-It). Phylogenetic analysis based on nucleotide sequences of CP genes showed that CMV-It clustered with CMV-K and belonged to subgroup I. To our knowledge, this is first identification of CMV in woad by RT-PCR and the CP gene was analyzed. This work provided data for research and control of woad mosaic disease.
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Objective To research the anti-influenza effect of active ingredients in Compound Yizhihao Granule (CYG). Methods The cytotoxicity and cytopathic effects (CPE) were observed under the phase-contrast microscope, besides 50% toxicity concentration (TC50) and 50% inhibitory concentration (IC50) were also calculated using Reed-Muench method, then the antiviral activity in vitro according to Selection Index (SI = TC50/IC50) was evaluated. In PR8 virus-infected mice, survival time, death rate, and lung index were observed in order to evaluate the protective effect. Besides, the effective ingredients were determined using HPLC method, and their contents were calculated by external standard method. Results CYG could inhibit the influenza virus-induced CPE, with IC50 of 4.6 mg/mL (equal to herbal extracts 262.2 μg/mL), and no direct cytotoxic effect at this concentration. PR8-infected mice were ig given CYG, the lung index and mortality were significantly reduced, and survival time was obviously prolonged. HPLC analysis indicated CYG contained many kinds of antivirus active components, including rupestonic acid, epigoitrin, and adenosine. Conclusion CYG is an effective natural anti-influenza medicine. Its antiviral effect should be the synergic effect of a variety of antiviral active ingredients.