Effects of different time on ischemia/reperfusion injury in isolated rat hearts / 中国药学杂志

METHODS: Male Sprague Dawley rats which were used lor Langendoff isolated heart perfusion were divided into four groups; normal control group (n=6), 120 group (n=1), 130 group (n=8) and 140 group (n=8), these hearts were subjected to global ischemia for 20, 30 and 40 min respectively. Then coronary flow, heart rate, creatinine kinase and lactate dehydrogenase in effluent and the changes of cardiac function parameters were measured in different groups. Infarct and risk areas were measured by planimetry using Image/J software.