ABSTRACT
Objective: To investigate the chemical constituents from the aerial parts of Leonurus macranthus. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Results: Nineteen compounds were isolated from the CH2Cl2 layer of 70% aqueous acetone extract in the aerial parts of L. macranthus, and identified as (+)-syringaresinol (1), (+)-1-hydroxysyringaresinol (2), rayalinol (3), erythro-guaiacylglycerol-β-O-4'-coniferyl ether (4), (7R, 7'R, 7″S, 8S, 8'S, 8″S)-3', 4″- dihydroxy-3, 5, 4', 5″-tetramethoxy-7, 9': 7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineo-lignan-7″, 9″-diol (5), (7R, 7'R, 7″S, 8S, 8'S, 8″S)-4', 5″- dihydroxy-3, 5, 3', 4″-tetramethoxy-7, 9': 7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineo-lignan-7″, 9″-diol (6), genkwanin (7), 3'-hydroxy- genkwanin (8), eriodictyol (9), isoscopoletin (10), p-coumaric acid (11), caffeic acid methyl ester (12), trans-ferulic acid (13), syringic aldehyde (14), vanillic acid (15), oct-1-en-3-yl β-glucopyranoside (16), 5-hydroxy-2-pyrrolidone (17), pterolactam (18), and nicotinamide (19), respectively. Conclusion: Compounds 1-6 and 9-19 are isolated from the plants of genus Leonurus Linn. for the first time, and compounds 7 and 8 are found from L. macranthus for the first time.
ABSTRACT
Objective To study the chemical constituents from Pericarpium Citri Reticulatae (Citrus reticulata). Methods The chemical constituents were isolated and purified by silica gel column, Sephadex LH-20, and ODS column chromatography. The structures were identified by spectral data. Results Nineteen compounds were isolated and identified as 4′,5,6,7-tetramethoxyflavone (1), 3,3′,4′,5,6,7,8-heptmethoxyflavone (2), sinensetin (3), 5-O-demethylnobiletin (4), tangeretin (5), nobiletin (6), apigenin (7), 5-O-desmethyltangeretin (8), 5,7-dihydroxy-3,3′,4′,6-tetramethoxyflavone (9), pachypodol (10), 4′,5,6,7-tetramethoxyflavanone (11), 3′,4′,5,7,8-pentamethoxyflavanone (12), agestricin C (13), scoparone (14), isoscopoletin (15), hesperidin (16), didymin (17), methylhesperidine (18), and naringin (19). Conclusion Compounds 9-15 are obtained from this plant for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from Gleditsiae Spina (the thorns of Gleditsia sinensis). Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatographic techniques, and their chemical structures were confirmed on the basis of spectroscopic analysis by the physicochemical properties. Cytotoxic activity using the MTT colorimetry method was performed to measure the inhibitory effect of the compounds on cell proliferation of HepG2, A-549, and EC109. Results: Three coumarins were obtained from the ethyl acetate soluble fraction of the 90% ethanolic extract and their structures were identified as scoparone (1), isoscopoletin (2), and 6-amino-7-methoxycoumarin (3). Conclusion: Compound 3 is a novel coumarin named gledisinmarin A. Compounds 1 and 2 are isolated from this plant for the first time. Compound 2 displays the stronger cytotoxicity against A549 cell with an IC50 value of 34.47 μg/mL, while cisplatin with an IC50 value of 11.50 μg/mL as a positive control.
ABSTRACT
Chemical investigations of the dichloromethane extracts of Ixora philippinensis Merr., a plant endemic to the Philippines, led to the isolation of syringaresinol (1), pinoresinol (2), isoscopoletin (3), squalene (4), β-sitosterol (5a), and stigmasterol (5b) from the stems; and 4, 5a, 5b, lupeol (6), and lutein (7) from the leaves. The structures of 1 and 3 were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 4-7 were identified by comparison of their NMR data with literature data.