ABSTRACT
The chemical constituents in stem leaf, root, and flower of Ixeris sonchifolia were identified by the ultra performance li-quid chromatography coupled with linear ion trap quadrupole-orbitrap mass spectrometry(UPLC-LTQ-Orbitrap-MS~n). The separation was performed on an Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) with a mobile phase of water(containing 0.1% formic acid, A)-acetonitrile(B) with gradient elution. With electrospray ionization source, the data of 70% methanol extract from stem leaf, root and flower of I. sonchifolia were collected by high-resolution full-scan Fourier transform spectroscopy, data dependent acquisition, precursor ion scan, and selected ion monitoring in the negative and positive ion modes. The compounds were identified based on accurate molecular weight, retention time, fragment ions, comparison with reference standard, Clog P and references. A total of 131 compounds were identified from the 70% methanol extract of I. sonchifolia, including nucleosides, flavonoids, organic acids, terpenoids, and phenylpropanoids, and 119, 110, and 126 compounds were identified from the stem leaf, root and flower of I. sonchifolia, respectively. In addition, isorhamnetin, isorhamnetin-7-O-sambubioside and caffeylshikimic acid were discovered from I. sonchifolia for the first time. This study comprehensively analyzed and compared the chemical constituents in different parts of I. sonchifolia, which facilitated the discovery of effective substances and the development and application of medicinal material resources of I. sonchifolia.
Subject(s)
Drugs, Chinese Herbal/chemistry , Methanol , Chromatography, High Pressure Liquid/methods , Mass Spectrometry , AsteraceaeABSTRACT
Objective To study thechemicalconstituentsof Ixerissonchifolia.Methods Monomericcompoundswerei‐solated by chromatography on silica gel column ,Sephadex LH‐20 ,ODS chromatography column and HPLC .Thestructure of the compound wasconfirmed on the basis of physio‐chemical constants and spectroscopic analysis .2 ,2‐diphenyl‐1‐pikrylhydra‐zyl (DPPH) and 3‐ethylbenzthiazoline‐6‐sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds .Results Five compounds were isolated from 70% ethanol‐water extraction and their structures were identified as (+ )‐pinoresinol‐4‐O‐β‐D‐glucopyranoside(1) ,glochidioboside(2) ,3 ,4 ,5‐trimethoxyphenyl‐1‐O‐β‐D‐glucopy‐ranoside(3) ,on‐onin(4) ,daidzein(5) ,luteolin (6) ,and apigenin (7) .Meanwhile ,antioxidant activities of all the isolates were evaluated by DPPHand ABTS .Conclusion Compounds 1‐5 were isolated from this genus for the first time .The active results showed that 1 ,2 ,4‐7 exhibited potent antioxidant activity .
ABSTRACT
AIM:To establish a HPLC diagitized fingerprint for Ixeris sonchifolia Hance Injection to serve as a digitized method criteria for its overall quality control. METHODS: The chromatographic fingerprints were obtained by injecting 20 ?L of the sample solution each time on a Century SIL BDS column (250 mm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of 1% acetic acid water and 1% acetic acid acetonitrile. The flow rate was 1 mL/min, the column temperature was maintained at (30?0.15)℃ and the detection wavelength was set at 265 nm. The chromatographic fingerprints were charateristically digitized and evaluated by the software of the digitized evaluation system of traditional Chinese medicine fingerprint with the super information characteristics to give the 42 parameters such as chromatographic fingerprint index (F), chromatographic fingerprint resolution index (RF), which could thoroughly disclose the hidden information in the fingerprints. Meanwhile, the apparent molecular weight, the peak position, the elution fore index and the pseudo peak area were marked in terms of the principle of QSPR when the two peaks were selected as the reference peaks. RESULTS: 21 co-possessing peaks were selected as the fingerprint peaks of Ixeris sonchifolia Hance Injection by taking caffeic acid peak as the reference peak to establish the digitized fingerprint and obtain the important digital information about its quanlity control. The stabilities among different batches of samples were evaluated by the dual qualitative and dual quantitative similarity method. CONCLUSION: This new digitized fingerprint method with good precision and reproducibility can be perfectly applied to the quality control over Ixeris sonchifolia Hance Injection. Digitized fingerprint obtained from the dual qualitative and dual quantitative similarity method maybe extend further to assess and control the quanlity of TCM.