ABSTRACT
Objective To establish an HPLC method for determination of 2,3,5,4’-tetrahydroxystibene-2-O-β-D-glucoside(SBGC)and hyperin in the effective fraction of Jiangzhining. Methods HPLC analysis was performed on a C18 column(4.6 mm×250 mm,5 μm),with acetonitrile-0.1% methanoic acid water solution(15.5: 84.5)serving as the mobile phase. The flow rate was 1.0 mL·min-1 ,the wavelength was 340 nm,and the injection volume was 20 μL. Results The calibration curve was linear for SBGC in the range of 24-120 μg·mL-1(r = 0.999 8)and for hyperin in the range of 2.4-12.0 μg·mL-1(r= 0.999 6),respectively.Their average recoveries were 100.07% and 100.14%,respectively.The contents of SBGC and hyperin were 2.26% and 0.23%,respectively Conclusion The method is convenient,precise and reliable for determination of the content of SBGC and hyperin in the effective fraction of Jiangzhining.
ABSTRACT
OBJECTIVE:To establish an HPLC method for the determination of the content of Quercetin in Jiangzhining capsules.METHODS:The chromatographic column was Shim-pack VP-ODS C18(150 mm?4.6 mm,5 ?m).The mobile phase was methanol-0.4% phosphoric(52∶48) at a flow rate of 0.8 mL?min-1.The detection wavelength was 373 nm.RESULTS:The linear range of Quercetin was 0.018 9～0.056 6 ?g(r=0.999 9),with its average recovery rate at 98.06%(RSD=1.73%,n=6).CONCLUSION:The method is simple,reliable and accurate,and suitable for the quality control of Quercetin in Jiangzhining capsules.