ABSTRACT
Objective: To study the effects of Jinqi Jiangtang Tablet (JQJT) in compatibility on the pharmacokinetics of berberine hydrochloride (BH) in rats. Methods: Male SD rats were ig administered with JQJT (0.42 g/kg, equivalently 5 mg/kg BH), BH (5 mg/kg) + chlorogenic acid (CA, 3 mg/kg), and BH (5 mg/kg), respectively. The plasma concentration at indicated time points were determined by LC-MS/MS method after the drug administrations. The pharmacokinetic parameters of BH were calculated with DAS 3.0 software using non-compartmental model. Results: After the drug administration, no significant change was found for the MRT0~∞, tmax, and t1/2 values of BH, whereas the AUC0~∞ and Cmax values of BH were in the order of BH group >JQJT group > BH + CA group, and the CLz and Vz values of BH were in the order of BH + CA group > JQJT group ≈ BH group. Conclusion: The pharmacokinetic results indicate that the co-administration of BH + CA decreases the absorpation, while increases the plasma clearance and distribution of BH in rats. Additionally, the results also suggest that the compatibility of astragalus root or honeysuckle with Coptidis Rhizoma is likely to produce the opposite effect on the plasma elimination and distribution of BH in vivo in rats.
ABSTRACT
AIM: To establish a reversed-phase HPLC method of determining three kinds of berberine type alkaloids(jatrorrhizine,palmatine and berberine) in Rhizoma Coptidis and Jinqi Jiangtang Tablet(Flos Lonicerae japonicae,Rhizoma Coptidis and Radix Astragali) METHODS: RP-HPLC was used for determination.The separation was carried out on Diamonsil C_(18)column(4.6 mm?250 mm,5 ?m) with the mobile phase containing acetonitrile-0.1 mol/L potassium dihydrogen phosphate(30(∶)70) and a detective wavelength was at 345 nm.The flow rate was 1.0 mL/min. RESULTS: The average contents of jatrorrhizine,palmatine and berberine in Rhizoma Coptidis and Jinqi Jiangtang Tablet were 1.34%,1.61%,7.12% and 0.35%,0.64%,1.54% respectively. CONCLUSION: The method is simple,accurate with a good recovery and repeatability and can be used to control the quality of Rhizoma Coptidis and Jinqi Jiangtang Tablet.