ABSTRACT
OBJECTIVE:To establish the quality method control of Jiufen spray,a percutaneous administration preparatio_n.METHODS:To determine the contents with TLCs method.The thin-layer plate was made of silica gel GF254. The spread out agent was chloroform-methanol-ammonia solution(4mol/L)(2∶6∶1). Dual wavelength scanning:strychnine and brucine:?s=254nm,?r=325nm;ephedrine:?s=500nm,?r=440nm.RESULTS:The within-day recoveries of strychnine,brucine and ephedrine were 99.048?2.388,99.532?0.958 and 99.504?0.555;the between-day recoveries were 98.796?1.058,99.58?0.476 and 99.42?0.838;the contents were(1.474?0.047)%,(1.372?0.052)%and(1.506?0.064)%(n=4) respectively.CONCLUSION:This method is stable and reliable and can be used for determination of the contents of this preparation.