ABSTRACT
Objective: To study the chemical constituents from Kalimeris indica. Methods: Chemical constituents were isolated by column chromatography and semi-prepared HPLC, and the structures were elucidated by spectral data and physical-chemical properties. Results: Fifthteen compounds were isolated and respectively identified as friedelin (1), epifriedelanol (2), stimasterol (3), β-sitosterol (4), α-spinasterol (5), erythodiol (6), octadecanoic acid (7), fraxinellone (8), β-daucosterol (9), hexacosanol (10), ergoaterol (11), sphondin (12), dibntyl phthalate (13), oleanic acid (14), and dammerdienly acetate (15). Conclusion: Compounds 5, 8-13 are isolated from K. indica for the frist time. Compounds 1, 3, 5, 8, and 12 have inhibitory effects on Cytospora sp. and Rhizoctonia solani.
ABSTRACT
Objective: The polysaccharide from Kalimeris indica (KIP) has been proved to be one of effective parts with anti-gastric ulcer action. In order to accurately define the structure and structure-activity relationship of KIP, the purification and structural analysis of KIP were carried out. Methods: A water-soluble polysaccharide (KIP-1) was obtained from K. indica by hot water extraction, deproteination with enzymolysis and Sevag methods, and fractionation through DEAE cellulose (DE-52) column. The molecular weight of KIP-1 was determined by gel chromatography (Sephadex G-200). The structure was characterized with FTIR, GC, and GC-MS methylation analysis. The microstructure of KIP-1 was observed by atomic force microscope (AFM). Results: The results indicated that KIP-1 had a molecular weight of 11 592 and was mainly composed of mannose, glucose, and galactose with the ratio of 2.523:1.162:1. Methylation analysis showed that KIP-1 had a backbone of 1, 3-linked glucose and 1, 2-linked galactose, of which the branch point was at O-3 composed of a 1, 3, 6-linked mannose residue and terminated with mannose residue. The microstructure study showed that KIP-1 with an average particle size of 1-2 nm was granular, irregular-rounded, and ellipsoidal. Irregularly shaped structure was found together in cubic image of KIP-1 with the height about 1 nm. Conclusion: KIP-1 with branch structure is mainly composed of mannose, glucose, and galactose. It is the first time to get the polysaccharide from K. indica.