ABSTRACT
Objective To evaluate the therapeutic effect of Kangbingdu oral liquid in combination with ribavirin aerosol for hand, foot, and mouth disease (HFMD). Methods A total of 135 HFMD children were enrolled and assigned to receive ribavirin aerosol alone (ontrol group, 67 patients) or ribavirin aerosol plus Kangbingdu oral liquid group (treatment group, 68 patients) for 7 days. Therapeutic effect was evaluated as the time to fever resolution, subsidence of skin rash and oral ulcers. All throat swab samples were tested using a fluorescence quantitative RT-PCR assay for panenteroviruses, enterovirus 71(EV71)and Coxsackievirus A16 (CA16). Results The time to fever resolution(2.51±0.42 d vs. 3.92±0.61 d;t=15.621, P<0.01), subsidence of skin rash(4.22±1.34 d vs. 6.33±1.41 d;t=8.914, P<0.01)and oral ulcers(3.01±0.32 d vs. 4.52±1.21 d;t=9.880, P<0.01)in the treatment group were significantly shorter than those in the control group. The healing rate in the treatment group were significantly higher than that in the control group(88.2%vs. 44.8%;χ2=26.761, P<0.01), and total effective rate was no different in treatment and control groups(94.1%vs. 83.6%;χ2=2.801, P=0.094). The rates of conversion from positive to negative for panenteroviruses(90.9% vs. 52.6%;χ2=13.331, P<0.01)and EV71(83.3%vs. 52.4%;χ2=4.307, P=0.038)in the treatment group were significantly higher than those in the control group, and the rate of conversion from positive to negative for CA16 was no different in treatment and control groups(93.3%vs. 81.8%;χ2=0.082, P=0.774). Conclusion Kangbingdu oral liquid in combination with ribavirin aerosol may improve the rate of conversion from positive to negative for enteroviruses, reduced the time to fever resolution, subsidence of skin rash and oral ulcers in HFMD children.
ABSTRACT
OBJECTIVE:To establish a TLCS method for the determination of Sarsa sa pogenin in Kangbingdu oral liquid.METHODS:TLCS was used with benzol-acetone(9 ∶1)as developing agent Sawtooth scanning was at ?S=443nm,?R=600nm RESULTS :The linear range was 1 028?g～3 624?g(r=0 9 995) The average recovery was 100.89%,and the RSD was 3 36%(n=5) The stability and precision were go od CONCLUSION:TLCS method can be used for the determination of Sarsasapogenin in Kangbingdu oral liquid
ABSTRACT
Objective To establish a method for the determination of chimoni and forsythiaside in Kangbingdu Oral Liquid(KOL) and to develop a new quality standard for KOL. Methods A RP-HPLC method was adopted for the determination of chimonin and forsythiaside of KOL. The chromatographic conditions were as follows:Kromasil RP-C18(250 mm? 4.6 mm,5 ? m) column was used,a mixture of acetonitrile -0.1 % acetic acid(15 ∶ 85,v/v) served as mobile phase,the flow rate was 0.80 mL? min-1,and detective wavelengths of time process were 0→ 25 min:258 nm,and 25→ 30 min:277 nm. Theoretical plate number of chimoni was over 6000 and that of forsythiaside was over 5000. Results The chimonin showed a good linearity in the range of 48.8ng～ 1525 ng(r=0.999 4),and the linear range of forsythiaside was in the ranged of 56ng～ 1750 ng(r=0.999 5).The mean recovery of chimonin and forsythiaside was 97.65 % and 99.21 % respectively. The content of chimonin in the 10 batches of samples was in the range of 14.40～ 20.41 ? g? mL-1,and that of forsythiaside was in the range of 28.70～ 36.01 ? g? mL-1. Conclusion The developed method has been proven to be simple,stable and reproducible,and can be applied for quality control of the Kangbingdu Oral Liquid.