ABSTRACT
Gynecological inflammatory disease refers to female reproductive system inflammatory disease, which has a direct impact on the female conception and reproductive health. Kangfuyan capsule is a classic traditional Chinese medicine preparation of the Miao nationality, which has the functions of promoting blood circulation, removing blood stasis, and clearing away heat and dampness. It is mainly used to treat pelvic inflammation, vaginitis, and chronic cervicitis caused by the accumulation of heat and dampness. In this paper, the basic research and clinical application of the Kangfuyan capsule in gynecological inflammatory diseases were reviewed to provide a reference for the development of drug for gynecological inflammation diseases.
ABSTRACT
OBJECTIVE To establish the fingerprints of Kangfuyan capsules and carry out chemical pattern recognition analysis，and simultaneously determine the contents of five components so as to promote the quality standard of the drug. METHODS High performance liquid chromatography （HPLC）fingerprints of 11 batches of Kangfuyan capsules （S1-S11）were established by Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）；identification and attribution analysis of chromatographic peaks were carried out by comparison with the chromatograms of the reference substance and the decoction pieces of single ingredient. SPSS 26.0 and SIMCA 14.1 software were used for cluster analysis and principal component analysis. HPLC method was used to determine the contents of matrine ，phellodendrine chloride ，rutin，forsythoside A and berberine hydrochloride. RESULTS There were 29 common peaks in the fingerprints for 11 batches of samples ，and the similarity was higher than 0.99. A total of 5 chromatographic peaks were identified ，which are matrine （peak 3），phellodendron chloride （peak 14），rutin （peak 20），forsythiaside A （peak 22） and berberine hydrochloride （peak 28）. The results of cluster analysis and principal component analysis showed that S 1-S9 were clustered into one category ，and S 10 and S 11 were clustered into another category. The contents of above 5 components were 29.320 5-60.144 3，0.621 6-1.076 6，1.025 9-2.830 5，2.899 3-6.212 7 and 4.425 1-8.581 6 mg/g， respectively. CONCLUSIONS The established fingerprint and content determination method are stable and reliable ，and can provide reference for the quality control of the preparation in combination with chemical pattern recognition analysis.
ABSTRACT
The aim of this paper was to explore the modern scientific connotation of Qizu Xueyu syndrome in Miao medicine and the intervention mechanism of Kangfuyan Capsules on Qizu Xueyu syndrome. Under the guidance of the theory of Miao medicine,an experimental animal model was constructed to characterize Qizu Xueyu syndrome in Miao medicine,and its modern scientific connotation was explored from the perspectives of general signs,blood lipid index,serum biochemical index and immune index. Furthermore,the relevant experimental animals were given Kangfuyan Capsules for intervention,then observed the corrective effect of Kangfuyan Capsules on abnormal physiological and biochemical indexes of the experimental animal model. The model animals showed signs such as mental depression,rough coat,dull and glossy,and the writhing behavior changed significantly. Low density lipoprotein cholesterol,creatine phosphate kinase,Ig G,urea nitrogen,creatinine,direct bilirubin,urea,urinary biliary and liver index were significantly higher than those in the blank group( P< 0. 01). The blood sugar and thymus index were significantly lower than those in the blank group( P <0. 01). After the intervention of Kangfuyan Capsules,the physiological and mental state of the experimental animals improved,their weight increased and their writhing behavior improved. Creatine phosphate kinase( CK) was significantly higher than that of Qizu Xueyu group( P<0. 01),direct bilirubin was significantly lower than that of Qizu Xueyu group( P<0. 01),immune indexes had no significant difference,urinary bilirubin returned to normal. The modern scientific connotation of Qizu Xueyu syndrome in Miao medicine can be characterized by ischemia,tissue and cell damage,infection and abnormal liver and kidney function caused by smooth muscle spasm. Kangfuyan Capsules can relieve uterine smooth muscle spasm,improve local blood circulation,repair muscle cell injury,protect liver and improve renal function.