Primary Study on the Chemical Constituents in Anticoagulating Active Fractions of Bombyx Batryticatus / 中国中医药信息杂志

Objective To explore the anticoagulating active components in water extraction, alcohol sedimentation and fraction separated by gelfiltration chromatography of Bombyx Batryticatus. Methods Anticoagulating active fractions were prepared by the methods of water extraction, 70% alcohol sedimentation and gelfiltration chromatography. Components (such as porteins or polypeptide, amino acids, oxalic acid ammonium) in each fraction were determined by chemical reaction indentification, Kjeldahl method, conductance, UV and HPLC. Results Polypeptide, amino acids and oxalic acid ammonium were the main components in anticoagulating active fractions, content of which reached more than 80%. The content of oxalic acid ammonium decreased about 52% processed by alcohol sedimentation, while the content of polypeptide and amino acids only decreased about 8%. The content of peptides and amino acids increased about 28% purified by gelfiltration chromatography compared with the one in water extraction and about 39% compared with the one in alcohol sedimentation. The content of oxalic acid ammonium was the same as the one in alcohol sedimentation, but decreased about 1 time compared with the one in water extraction. The content of 15 kinds of amino acids were 40%～50% in the solids of each fractions, which maked up to 60%～76% of the content of proteins determined by Kjeldahl method. Molecular weight range of peptides is 1.0～4.4 kDa. Conclusion This study provides a experimental basis for further separation and purification of anticoagulating active components from Bombyx Batryticatus.

Experimental studies on the alteration of NPN (non-protein nitrogen) with cold / 日本温泉気候物理医学会雑誌

Experiments to determine the relationship between the exposure time following death from cold and the amount of residual NPN in blood and organs.<br>Sixty rabbits weighing about 2500 grams were used in these experiments. Their whole bodies except the head and face were immersed in water at a temperature of 2 to 10°C and fastened by tight binding. Their rectal temperatures were measured five minutes apart using a thermocouple thermometer. The measured amount of residual NPN in tissues and blood with each experiment group by the Kjeldahl method after the death are as follows:<br>1) The amount of residual NPN in organs and blood differed depnding on aggressiveness and the length of time until death.<br>2) When the length of time until death was relatively short, the amount of NPN in organs decreased and that in blood increased.<br>3) When the length of time until death was relatively long, the amount of NPN inorgans increased extremely and that in blood also increased.<br>4) The amount of residual NPN in organs and blood starts to change at the initial stage of aggression.