ABSTRACT
Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of six components in Ilex kudingcha by HPLC. Methods The purity of kaempferol-3-O-β-D-rutanoside, isorhamnetin-3-O-β-D-rutanoside, kudinoside C, kudinoside A, kudinoside E, and kudinoside D was determined by HPLC-MS in test sample taking kudinoside C as internal standard, relative correction factors of the other five compounds were determined, and their contents were calculated. At the same time, the contents of these six components were determined by external standard method (ESM), and the difference between ESM and QAMS was compared to verify the feasibility and accuracy of QAMS method. Results There was no significant difference of the six components’ contents calculated between QAMS method and ESM method. Conclusion It is convenient, feasible, and accurate to measure the content of six components in I. kudingcha by QASM method.
ABSTRACT
Objective: To establish an HPLC method for simultaneously determining latifoloside G, latifoloside H, kudinoside G, latifoloside C, kudinoside A, kudinoside E, and kudinoside D in Ilex kudingcha. Methods: The separation was performed on a YMC C18 column (YMC-pack ODS-A, 250 mm × 4.6 mm, 5 μm) with water-acetonitrile as the mobile phase in a gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 30℃. Results: Seven triterpenoid saponins all have good separating degree, good linear range, and linearity (r > 0.999 5). The average sample recovery rates were 99.25%-100.8%, RSD < 2%. Conclusion: The established method is rapid, accurate, and with has repeatability and stability, which could provides the scientific evidence for the quality control of I. kudingcha.