ABSTRACT
OBJECTIVE: To study the chemical constituents of Kummerowia striata. METHODS: The chemical constituents were isolated and purified with silica column chromatography, gel chromatography, etc. Their structures were identified by physicochem-ical properties and various spectroscopic methods including NMR spectrum, MS, UV, etc. RESULTS: Fourteen compounds were isolated and elucidated as quercetin-3-O-α-L-rhao pyranoside(1), kaempferol-3-O-α-L-rhamopyra noside(2), apigenin(3), quercetin(4), quercetin-3-O-β-D-galactopyranside(5), luteolin-7-O-β-D-glucoside (6), luteolin(7), campherol-3-O-D-sophoroside(8), isoorientin (9), orientin(10), apigenin-7-O-β-D-glucuronide methylester(11), 7-hydroxy-4′-methoxy isoflavone(12), tricin(13), and tricin-7-O-β-D-glucopyranoside(14). CONCLUSION: Compounds 1, 2, 5, 6, 8, 9, and 10-14 are isolated from Kummerowia striata for the first time.
ABSTRACT
Objective To examine the therapeutic effect of Kummerowia striata (KS) on IgA nephropathy (IgAN) rats.Methods Sixty SD rats were equally randomized into 6 groups after adaptive feeding for 3 days:blank control group,model control group,tripterygium glycosides (TG) group (in the dose of 0.0165 g/kg),and high-,middle-and low-dose KS groups (in the dose of 16.0,8.0,4.0 g/kg respectively).IgAN rat model was established with bovine serum albumin (BSA),lipopolysaccharide (LPS) and carbon tetrachloride.After modeling,rats except those in blank control group received gastric gavage of corresponding drugs for 10 continuous weeks.Levels of 24-hour proteinuria and hematuria in each group were measured,serum levels of total protein (TP),serum albumin (ALB),aspartate aminotransferase (AST),and alanine aminotransferase (ALT) were tested.The pathological changes of renal tissue were observed under light microscope by HE staining.Results Water decoction of KS could significantly decrease the proteinuria and hematuria levels,and reduce renal pathological changes of IgAN model rats.However,water decoction had no effect on indicators of liver function such as TP,ALB,AST,and ALT in IgAN rats.Conclusion KS has certain therapeutic effect on IgAN model rats.