ABSTRACT
［ Objective:To establish a HPLC method for examination of L-carnosine，eyeseryl，glutathione and acetyl hexapeptide-8 in cosmetics. Method:After being extracted by water， L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 were examined by HPLC with methanol-0.01% formic acid （V/V） aqueous solution as the mobile phase. The column was Agilent Eclipse XDB-C18 （5 μm， 4.6 mm×150 mm）. The flow rate was 0.5 mL/min. The determination wavelength was set at 210 nm. Results:There was a good linear relationship within the range of 5-100 μg/mL for L-carnosine， eyeseryl， glutathione， and acetyl hexapeptide-8. The recoveries of L-carnosine Eyeseryl Glutathione and Acetyl Hexapeptide-8 were between 92.5%~105.9%， with a RSD from 0.5% to 3.5%. Conclusion:The method is simple， sensitive， specific and reproducible in the examination of L-carnosine， Eyeseryl， Glutathione， and Acetyl Hexapeptide-8 in cosmetics.
ABSTRACT
Aim: To study the protective effect of L-carnosine on deguelin-induced neurotoxicity. Methods: SH-SY5Y cells were treated with 1, 10, 20, 40, 60, 80, 100 mmol · L" L-carnosine for 24 h; cell viability was detected by CCK-8 assay. SH-SY5Y cells were respectively treated with 30 mmol · L-1 L-carnosine, 20 μmol · L-1 deguelin, 20 μmol · L-1 deguelin with 30 mmol · L-1 L-carnosine for 24 h; AO/EB method was employed for observing the morphology and apoptotic morphology of treated cells. SH-SY5Y cells were respectively treated with 8 μmol · L-1 deguelin, 8 μmol · L-1 deguelin with 3 mmol · L-1 L-carnosine, 8 μmol · L-1 deguelin with 30 mmol · L-1 L-carnosine, 30 mmol · L-1 L-carnosine, 20 μmol · L-1 deguelin, 20 μmol · L-1 deguelin with 3 mmol · L-1 L-carnosine, 20 μmol · L-1 deguelin with 30 mmol · L-1 L-carnosine, 50 μmol · L-1 deguelin, 50 μmol · L-1 deguelin with 3 mmol · L-1 L-carnosine, 50 μmol · L-1 deguelin with 30 mmol · L-1 L-carnosine for 24 h. Cell proliferation was detected by CCK-8 assay; apoptotic rate of treated cells was determined by flow cytometry; the reactive oxygen species (ROS) of treated cells was detected by DCFH-DA staining flow cytometry. Results: After 30 mmol · L-1 L-carnosine was co-treated with 20 μmol · L-1 and 50 μmol · L-1 of deguelin, the cell inhibitory rate decreased by 9. 07% and 6. 1%, the number of early apoptotic cells decreased, and the early apoptotic rate decreased by 9. 35%. The total apoptotic rate decreased by 10. 7%, and the ROS level was lower than that of the deguelin alone treatment group. The difference was statistically significant (P < 0. 05). Conclusions: L-carnosine can effectively reduce the neurotoxicity of deguelin-induced SH-SY5Y cells, which may be related to the reduction of oxidative stress levels, thereby inhibiting apoptosis and protecting cells.
ABSTRACT
Objective To study the L-carnosine induced apoptosis of human hepatocellular carcinoma HepG2 cells and its mechanism.Methods Human hepatocellular carcinoma HepG2 cells were cultured in vitro,then the cells in logarithmic phase were divided into 5 mM,20 mM,50 mM L-carnosine group and control group,treated with 5 mM,20 mM,50 mM L-carnosine and saline respectively.The proliferation of HepG2 cells was measured by CCK-8 assay after 24 hours and 48 hours treatment.The cell apoptosis and cell cycle were detected by flow cytometry after 48 hours treatment.The protein expression of Caspase-8,PI3K and Akt were detected by Western blot in each group after 48 hours treatment.Results CCK-8 and flow cytometry assay showed that both 20 mM and 50 mM L-carnosine treated group significantly inhibited the proliferation of HepG2 cells after 24 hours and 48 hours,and the inhibition were in a time and dose-dependent manner.Western blot revealed that the expressions of PI3K and Akt were down-regulated with the increase of L-carnosine concentration,while the expression of Caspase-8 was increased.Conclusion L-carnosine exhibits an inhibitory effect of the proliferation and promote apoptosis of human liver cancer cell line HepG2.The mechanism may be associated with the inhibition of the PI3K/Akt signaling pathway and activation of Caspase-8 signaling pathway.
ABSTRACT
alpha-Lipoic acid and L-carnosine are powerful antioxidants and are often used as a health supplement and as an ergogenic aid. The objective of this study was to investigate the effects of alpha-lipoic acid and/or L-carnosine supplementation on antioxidant activity in serum, skin, and liver of rats and blood lipid profiles for 6 weeks. Four treatment groups received diets containing regular rat chow diet (control, CON), 0.5% alpha-lipoic acid (ALA), 0.25% alpha-lipoic acid + 0.25% L-carnosine (ALA + LC), or 0.5% L-carnosine (LC). Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and lipid peroxidation products, malondialdehyde (MDA) concentrations, were analyzed in serum, skin, and liver. Blood lipid profiles were measured, including triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C). Skin and liver SOD activities of the ALA and LC groups were higher than those of the CON group (P < 0.05), but serum SOD activity was higher only in the LC group compared to that in the CON group (P < 0.05). Additionally, only liver GSH-Px activity in the LC group was higher than that of the CON and the other groups. Serum and skin MDA levels in the ALA and LC groups were lower than those in the CON group (P < 0.05). Serum TG and TC in the ALA and ALA + LC groups were lower than those in the CON and LC groups (P < 0.05). The HDL-C level in the LC group was higher than that in any other group (P < 0.05). LDL-C level was lower in the ALA + LC and LC groups than that in the CON group (P < 0.05). Thus, alpha-lipoic acid and L-carnosine supplementation increased antioxidant activity, decreased lipid peroxidation in the serum, liver, and skin of rats and positively modified blood lipid profiles.
Subject(s)
Animals , Rats , Antioxidants , Cholesterol , Cholesterol, HDL , Cholesterol, LDL , Diet , Glutathione Peroxidase , Lipid Peroxidation , Lipoproteins , Liver , Malondialdehyde , Skin , Superoxide Dismutase , Thioctic Acid , TriglyceridesABSTRACT
0.05).Conclusions Early injection of L-carnosine would not only improve cerebral oxidative phosphorylation,relieve neuronal injury of repeated FS,but play a role in the protection of neuronal cells.
ABSTRACT
Objective To explore the effect of L-carnosine on neuronal cell apoptosis in young rats with experimental febrile seizures(FS).Methods Forty 15-day SD rats were randomly divided into intervention group(n=30)and FS group(n=10).Warm water was used to induce 10 times FS.The intervention group was divided into E,G and H group,10 rats in each group.Intraperitoneal injection of L-carnosine(250 mg/kg)was separately given to the rats in E group,G group and H group respectively after 30,60 and 120 min of seizure.FS group were induced FS,but they were not given intervention.The rats were sacrificed at 12 hours after the last seizure.Neuronal cell apoptosis was determined by terminal eoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)in situ cell death kit.TUNEL positive cells were stained and counted as apoptosis in hippocampus and cortex.Ultrastructural changes of apoptosis neurons were observed under the electron microscope.Results The neuronal cells apoptosis count was 25.37?1.95 in FS group,12.36?1.13 in E group,17.85?2.04 in G group,and 22.69?2.69 in H group.Neuronal apoptosis of FS group was apparently higher than that of interventional groups(F=10.75 P0.05).Under the electron microscope,neuronal damage on hippocampal CA1 area and dentate gyrus of FS group and H group was obviously higher than that of E group.Conclusions Early injection of L-carnosine would not only relieve neuronal apoptosis of repeated FS,but also play a role in the protection of neuronal cells.
ABSTRACT
OBJECTIVE:To develop an HPLC method for the determination of L-Carnosine in Polaprezine granules.METHODS:The Chromatographic column was Agilent ODS with detection wavelength of 215 nm and flow rate of 1 mL?min-1.The mobile phase consisted of sodium subsulfite buffer solution(pH=3)-acetonetrile(77:23).The column temperature was set at 30 ℃ and injection size was 10 ?L.RESULTS:The good linearity was obtained in the range of 0.1559～3.1584 ?g for L-Carnosine(r=1.0000).The average recovery was 99.65%(RSD=0.078%).CONCLUSION:The method is simple,accurate and sensitive,and it is suitable for content determination of L-Carnosine in Polaprezine granules.