ABSTRACT
Objective To investigate the cflecl of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4., spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OFCs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method. The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situ hybridization were observed in L4-J spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased(P<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord significantly increased(PcO. 05) compared with the CCI group, the reflex threshold of mcchaniral withdrawl of rats in the OF.Cs group was significantly increased (/'cO. 05). The percentage and average absorbance of P2X7 receptor positive cells in L«., spinal cord significantly decreased compared with the OECs group(P<0. 05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher(/,<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord were lower(H<0. 05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain belter and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.
ABSTRACT
STUDY DESIGN: We have analyzed the laparoscopic lateral retroperitoneal approach of the L4-5 interspace to the miniopen retroperitoneal approach for lateral lumbar interbody fusion. OBJECTIVES: To prospectively compare the laparoscopic lateral retroperitoneal approach of the L4-5 interspace to the miniopen retroperitoneal approach for lateral lumbar interbody fusion Summary of Background Data : The introduction of laparoscopic techniques in 1993 has stimulated a great deal of discussion regarding the risks and benefits of such minimally invasive approaches. In many centers the anterior endoscopic approach to L5-S1 has become routine. However exposure at L4-5 can be much more difficult. MATERIALS AND METHODS: From 1997 to 1999 thirty eight patients were entered into a prospective study. These patients were all undergoing anterior interbody fusion at the L4-5 level. The patients were divided into two groups for analysis. Group I patients underwent anterior interbody fusion utilizing threaded interbody devices placed via laparoscopic lateral retroperitoneal approach. Group II patients underwent anterior lumbar interbody fusion using threaded interbody devices placed via a miniopen retroperitoneal approach. RESULTS: In Group I, Operation time was 48 minutes longer than Group II (p=0.035) but there were no significant statistical differences in bleeding amount and hospitalization period. Parethesia and tingling sensation of thigh were developed in two cases of Group I patients, one case of Group II patients but they were gradually diminished. In Group I, only one cage was inserted in five cases of patients (28%) who had an inadequate exposure of L4-5 area. However, all of the patients in Group II (100%) had an adequate exposure of L4-5 area. CONCLUSION: The surgical results of laparoscopic technique was not superior to miniopen technique.