ABSTRACT
Objective:To investigate the expression of LACTB and GST-π in endometrial carcinoma and their correlation with estrogen and progesterone receptors.Methods:A total of 165 patients with endometrial cancer admitted to our hospital from Jan. 2015 to Oct. 2020 were selected to observe the expression of LACTB, GST-π, estrogen and progesterone receptors in patients with different tumor stages, degrees of differentiation, muscular infiltration, tissue type, tumor diameter and whether there was lymph node metastasis. The correlation between the expression of LACTB and GST-π and the expression of female and progesterone receptors and the survival of patients with different expressions of LACTB and GST-π were analyzed.Results:The LATCB positive rate decreased in patients with tumor stage III to IV, high differentiation, myometrial infiltration ≥1/2, tissue type I, tumor diameter <2 cm and no lymph node metastasis ( P<0.05), and the GST-π positive rate was not statistically significant ( P>0.05). The positive rate of GST-π in patients with chemotherapy resistance was higher than that in patients with chemotherapy sensitivity, and the difference was statistically significant ( P<0.05). The positive rate of estrogen receptor decreased in patients with tumor stage III to IV, high differentiation, tissue type II, no lymph node metastasis, and chemotherapy resistance, and the difference was statistically significant ( P<0.05). The positive rate of progesterone receptor decreased in patients with tumor stage III to IV, low differentiation, tissue type I and no lymph node metastasis, and the difference was statistically significant ( P<0.05). In this study, 110 patients with LACTB positive expression were detected, with an average LACTB positive staining intensity (25.92±4.77) %, and 99 patients with GST-π positive expression were detected, with an average GST-π positive staining intensity (27.46±4.83). A total of 50 patients with LACTB positive and GST-π negative had an average survival time of (41.48±5.52) months, a total of 39 patients with LACTB negative and GST-π positive had an average survival time of (21.25±3.15) months, and a total of 60 patients with LACTB positive and GST-π positive had an average survival time of (21.25±3.15) months. The mean survival time of 16 patients with both LACTB negative and GST-π negative was 29.31±3.77 months. The mean survival time was 31.54±4.61 months. Pearson correlation test showed that the staining intensity of LACTB positive staining was positively correlated with the survival time of patients. The positive staining intensity of GST-π was negatively correlated with survival (correlation coefficient =0.392, -0.284, P<0.01). Pearson correlation analysis showed that LACTB was positively correlated with estrogen and progesterone receptors. GST-π was negatively correlated with estrogen receptor ( P<0.05) . Conclusions:The expression of LACTB in patients with endometrial cancer is associated with the disease of the patients. The survival of patients with high expression is longer. The expression of GST-π is associated with the chemotherapy resistance of patients, both of which can be used as indicators to evaluate the prognosis of patients. Moreover, the expressions of LACTB and GST-π are correlated with the expression of female and progesterone receptors, which may be regulated by the expression of female and progesterone receptors.
ABSTRACT
Objective: To investigate the expression level of lactamase β (LACTB) in gastric cancer and its influence on the prognosis of gastric cancer patients. Methods: The varied expression of LACTB in 90 pairs of gastric cancer tissue and peritumor tissue was analyzed by immunohistochemistry. The prognostic role of LACTB mRNA in gastric cancer was analyzed by the Kaplan-Meier Plotter database. The factors affecting the overall survival of gastric cancer patients were analyzed with Cox survival model. The prognostic nomogram model was established to predict overall survival of gastric cancer patients. The proteins which interacted with LACTB were analyzed by the STRING database, and they were tagged by the notable associated functional cluster of GO (gene ontology) and KEGG (Kyoto encyclopedia of genes and genomes) pathway. Results: The expression of LACTB in gastric cancer tissue was obviously lower than that in peritumor tissue (P=0.000). The low expression of LACTB mRNA may suggest that the prognosis of gastric cancer patient is poor, and the C-index of nomogram for predicting prognosis of gastric cancer patients was 0.641. A set of LACTB and 38 proteins which interacted with LACTB participated in five kinds of signaling pathway. Results for the classification of GO (gene ontology) annotation demonstrated that the proteins were involved in one kind of cellular component, nine kinds of molecular function and two kinds of biological process. Conclusion: The low expression of LACTB may be a poor-prognostic factor in gastric cancer.