The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs / 中华创伤杂志(英文版)

<p><b>PURPOSE</b>Microgravity is known to cause endothelium dysfunction in astronauts returning from spaceflight. We aimed to reveal the regulatory mechanism in alterations of human endothelial cells after simulated microgravity (SMG).</p><p><b>METHODS</b>We utilized the rotary cell culture system (RCCS-1) to explore the subsequent effects of SMG on human umbilical vein endothelial cells (HUVECs).</p><p><b>RESULTS</b>SMG-treated HUVECs appeared obvious growth inhibition after return to normal gravity, which might be attributed to a set of responses including alteration of cytoskeleton, decreased cell adhesion capacity and increased apoptosis. Expression levels of mTOR and its downstream Apaf-1 were increased during subsequent culturing after SMG. miR-22 was up-regulated and its target genes SRF and LAMC1 were down-regulated at mRNA levels. LAMC1 siRNAs reduced cell adhesion rate and inhibited stress fiber formation while SRF siRNAs caused apoptosis.</p><p><b>CONCLUSION</b>SMG has the subsequent biological effects on HUVECs, resulting in growth inhibition through mTOR signaling and miR-22-mediated mechanism.</p>

MicroRNA-506 inhibits malignancy of colorectal carcinoma cells by targeting LAMC1 / 实用医学杂志

Objective To investigate the effects of microRNA-506 (miR-506) on malignant phenotype of colorectal carcinoma cells. To identify the target gene of miR-506 in colon carcinoma. Methods SW480 cells were divided into five groups, known as normal cell group, miR-506 overexpression and, miR-506 inhibition groups with their vehicle groups.The migration and invasion abilities of SW480 cells were measured with Transwell migration assay. Cell viability and colony forming activities were measured by CCK8 and colony formation assays, respectively. Furthermore, bioinformatic method, green fluorescent protein (GFP) reporter assays, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were applied to predict potential target genes of miR-506. Results The number of migrated and invasive cells, viability and clonality in the miR-506 overexpression groups reduced. LAMC1 mRNA and protein levels in the miR-506 overexpression groups were lower than those in the control groups. Conclusion LAMC1 is a direct target gene for miR-506 and miR-506 could inhibit the cell migratioin and invasion.