ABSTRACT
Objective: To analysis the main chemical ingredients of Forsythiae Fructus by LC-MS technology in the positive and negative ions mode. Methods: The chromatographic fingerprint was obtained with Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) and gradient elution with 0.05% H2O-formic acid (A)-acetonitrile (B), and the flow rate was 1.0 mL/min. The column temperature was maintained at 35℃. The detection wavelength was 200-600 nm. Positive and negative ions MS information of Bruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with literature data to analyze the compounds information. Results: Combined with the accurately relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 24 compounds were identified from the ethanolic extraction of Forsythia Fructus, which include 12 phenylethanoid glycosides, seven lignan ingredients, and five flavonoids. And 3,4-dihydroxyphenylethanoid glycoside (1), 2-methoxy-3,4,5-trihydroxyphenylethanoid glycoside (2), β-hydroxyforsythoside H (7), dihydroquercetagetin (24), and dihydromyricetin (25) were first identified in Forsythiae fructus. Conclusion: The method provides the technical support for the quality control of Forsythiae Fructus and contributes the reference data to elucidating the potential basis of Forsythiae Fructus.
ABSTRACT
Objective: To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS. Methods: The HPLC separation achieved on Agilent 1200 HPLC Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of 0.05% H2O-formic acid (A) and acetonitrile (B), the flow rate was 1.0 mL/min, the column temperature was 35℃, and the detection wavelength was 200-600 nm. Positive and negative ions MS information and element analysis, via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Results: Twenty-one chemical constituents were identified, including three phenolic acid, four flavonoids, and fourteen triterpenoid saponins, and isochlorogenic acid A (5), isochlorogenic acid B (6), 7,3'-di-O-methylquercetin (8), and 5-hydroxy-7-acetoxyflavone (24) were separated from Bupleuri Radix for the first time. Conclusion: It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS, which could provide the references for the application development.