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1.
Indian J Pathol Microbiol ; 2013 Apr-Jun 56 (2): 114-119
Article in English | IMSEAR | ID: sea-155843

ABSTRACT

Background: There are several methods for counting platelets, of which the international fl ow reference method (IRM) is considered to be the gold standard. We compared the platelet count given by this method to the count given by automated analyzers using other methods, such as optical fl uorescence and impedance. Aims: The aim of this study is to compare the platelet counts obtained by Sysmex XE 2100 by Impedance (Sysmex-I), optical fl orescence (Sysmex-O) and reported (Sysmex-R) based on the switching algorithm and LH-750 by Impedance (LH-750) with the IRM in thrombocytopenic blood samples. To calculate the sensitivity, specifi city, positive predictive value (PPV) and negative predictive value (NPV) of various technologies at the clinically relevant transfusion thresholds of 10 × 109/l and 20 × 109/l. Materials and Methods: A total of 118 blood samples with platelet count of <50 × 109/l were selected for the study. Platelet counts of all samples were analyzed by all methods using the Sysmex analyzer, LH-750 and IRM in parallel within 6 h of collection. Statistical Analysis Used: Pearson correlation, bland Altman analysis, sensitivity and specifi city, PPV and NPV. Results and Conclusions: Sysmex-R had the least Bias and 95% limits of agreement (95%LA) range and thus correlated best with IRM values. LH-750 had a higher Bias compared to Sysmex-O and Sysmex-R, but a strikingly similar 95% LA ensures similar results in all three methods. In fact, in the oncology subset, it had the narrowest 95% LA, which made it the best performer in this subgroup. Of the three Sysmex results, Sysmex-I had the highest bias, widest 95% LA and highest potential risk of over transfusion. Hence, Sysmex-R and LH-750 were found to be reliable tools for estimation of platelet count in thrombocytopenic patients.

2.
Journal of Laboratory Medicine and Quality Assurance ; : 173-181, 2005.
Article in Korean | WPRIM | ID: wpr-68688

ABSTRACT

BACKGROUND: Our aim was to set reference intervals of healthy adults using Beckman Coulter LH 750 by gender and age. METHODS: The specimens were obtained from a total of 705 healthy adults (male 484, female 221), who took part in annual health-check at Chungnam National University Hospital, analyzed in total 22 parameters and compared using SPSS V10.0 program. RESULTS: Totally 16 parameters showed the Gaussian distribution with 12 in parametric method and 4 in logarithmically transformed parametric method. All acquired reference intervals were showed in Table 3, 4, 5 and 6. There were statistical significances between genders in RBC, Hgb, Hct, MCV, MCH, WBC, EO%, LY#, MO#, EO#, MPV, PDW (P<0.001), BA% (P=0.001), NE% (P=0.016), BA# (P=0.019), MO% (P=0.021) and NE# (P=0.039), between age decades in RBC, Hgb, Hct, MCV, MCH, NE%, LY% (P<0.001), LY# (P=0.002), EO%, NE# (P=0.003) and Pct (P=0.033) as well as between genders and age decades in RBC, Hct (P=0.001), Hgb (P=0.004), LY# (P=0.005), Plt (P=0.014) and MO% (P=0.017). CONCLUSIONS: This study suggested that the reference intervals of RBC and Hgb ought to be set by both genders and age decades, WBC by gender and the others by total study populations. Moreover, it need to be set the reference intervals by each laboratory for itself and to be monitored with periodic review.


Subject(s)
Adult , Female , Humans , Cell Count , Normal Distribution
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