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This study aimed to explore the effect of Salviae Miltiorrhizae Radix et Rhizoma, its stems and leaves on the diversity of intestinal microflora in rats with diabetic kidney injury. Diabetic rats model was established by feeding high glucose and high fat diet and 5% glucose solution with intraperitoneal injection of 30 mg·kg~(-1) streptozocin(STZ). The rats were randomly divided into normal group, model group, irbesartan control group, Huangkui Capsules control group, as well as low, middle and high dose groups of Sal-viae Miltiorrhizae Radix et Rhizoma, its stems and leaves. After administration for 2 weeks, 16 S rRNA technique was used to analyze the diversity of intestinal microflora in the feces of each group. The results showed rats in the model group developed renal tubular epithelial vacuole degeneration and a large amount of inflammatory cell infiltration in the renal interstitium. A small amount of inflammatory cell infiltration was seen in each administration group. The kidney structure of rats in irbesartan group, Huangkui Capsules group, high-dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem water extract, as well as high dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem ethnol extract group was close to the normal group. The diversity and structure of intestinal flora in the model group were significantly different from those in the normal group. Each administration group improved the fecal flora diversity in rats with diabetic kidney injury to a certain extent, especially the high dose of Salviae Miltiorrhizae Radix et Rhizoma and its stems water extract. Different flora were found in feces of diabetic nephropathy model rats on class, order, family and genus levels. On families and genera levels, the relative abundance of Bifidobacterium, Turicibacter, Peptostreptococcaceae, Desulfovibrio, and SMB53 showed an upward trend in model group, but that of Lactobacillus, Clostridium, Rikenella, Rumen fungi showed a downward trend. The administration groups can improve the relative abundance of the above intestinal flora in the model rats to a normal-like level. The results of this study provide a reference for resource utilization and further development of Salviae Miltiorrhizae Radix et Rhizoma.
Subject(s)
Animals , Rats , Diabetes Mellitus, Experimental/drug therapy , Diabetic Nephropathies/drug therapy , Drugs, Chinese Herbal , Gastrointestinal Microbiome , Salvia miltiorrhizaABSTRACT
Objective: To evaluate the efficiency of ITS2 and matK DNA barcode for the identification the Labiatae medicinal plants in Emei Mountain. Methods: A total of 23 samples of Labiatae medicinal plants were harvested in Emei Mountain. The DNA was extracted and used for PCR to obtain the ITS2 sequences. Meanwhile, 54 ITS2 and 51 matK sequences of the medicinal plants in Labiatae were downloaded from Genbank. The interspecies and intraspecific genetic distance and sequence variation sites of all sequences were determined by MEGA 5.0 software. The Neighbor Joining (NJ) phylogenetic tree was constructed, and barcoding gap analysis was then performed. Results: The length of various ITS2 sequence was distributed from 190 to 237 bp, with GC content of 53%-73%. Moreover, significant barcoding gap was observed when comparing the distances, the recognition rate for plants of the Labiatae family was 94%.The barcoding gap of the matK sequence was not significant, there was obvious overlap, and the recognition rate for the Labiatae family was 96%. Conclusion: ITS2 has a better ability to identify Lamiaceae plants at the species level, but the matK sequence has a higher recognition rate for Lamiaceae plants. Therefore, the employment of ITS2 as core with matK as supplement was able to identify Lamiaceae plants quickly and accurately, and understand the genetic relationship between species accurately. This provides an important theoretical basis for the effective protection and rational development of the medicinal plant resources of the Labiatae plants in Emei Mountain.
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Abstract: The Lamiaceae family includes about 7,000 species allocated in 236 genera, with almost cosmopolitan distribution, currently divided into nine subfamilies. In Brazil 46 genera and 525 species occur, distributed in four subfamilies. Among these subfamilies, Ajugoideae and Viticoideae were originally parts of the Verbenaceae family, being transferred to Lamiaceae as a result of several systematic studies on the two families. Ajugoideae is characterized by having drupes with four mericarps, non-persistent styles and pollen grains with exine formed by granular and branched columns. On the other hand, Viticoideae is characterized to have drupes with 4-locular pyrenes or 2 2-locular pyrenes and exine microreticulate or slightly rough. We present here the results of a taxonomic survey of the subfamilies Ajugoideae and Viticoideae (Lamiaceae) for Paraíba State, Brazil, based on excursions to collect fertile material, analyses of specimens incorporated into herbaria in Paraíba (ACAM, CSTR, EAN and JPB) and Pernambuco State (IPA and PEUFR), and consultations of the SpeciesLink and Herbário Virtual REFLORA databases. Identifications were based on the specialized literature and the examination of type and protolog specimens. Ten species belonging to three genera, five belonging to the subfamily Ajugoideae (Aegiphila integrifolia (Jacq.) Moldenke, A. luschnathii Schauer, A. pernambucensis Moldenke, A. verticillata Vell. and Amasonia campestris (Aubl.) Moldenke) and five to the subfamily Viticoideae (Vitex gardneriana Schauer, V. megapotamica (Spreng.) Moldenke, V. orinocensis Kunth, V. rufescens A. Juss. and V. schaueriana Moldenke) were recorded. The taxonomic treatment includes a key to identify genera and species, descriptions, illustrations, taxonomic comments, geographic distribution, habitats and phenological data of the species.
Resumo: A família Lamiaceae engloba cerca de 7.000 espécies alocadas em 236 gêneros, com distribuição quase cosmopolita, atualmente dividida em nove subfamílias. No Brasil ocorrem 46 gêneros e 525 espécies, distribuídas em quatro subfamílias. Dentre estas subfamílias, Ajugoideae e Viticoideae eram originalmente partes da família Verbenaceae, sendo transferidas para Lamiaceae como resultado de diversos estudos sistemáticos sobre as duas famílias. Ajugoideae caracteriza-se por possuir drupas com quatro mericarpos, estiletes não persistentes e grãos de pólen com exina formada por colunas granulares e ramificadas. Por outro lado, Viticoideae caracteriza-se por possuir drupas com um pirênio 4-locular ou 2 pirênios 2-loculares e exina microrreticulada ou levemente rugosa. O presente trabalho compreende o levantamento taxonômico das subfamílias Ajugoideae e Viticoideae (Lamiaceae) para o estado da Paraíba, Brasil. O estudo foi baseado em espécimes coletados em campo, na análise de coleções depositadas nos herbários ACAM, CSTR, EAN, IPA, JPB e PEURF) e na consulta às bases de dados SpeciesLink e Herbário Virtual REFLORA. Foram registradas 10 espécies em três gêneros, sendo cinco pertencentes à subfamília Ajugoideae (Aegiphila integrifolia (Jacq.) Moldenke, A. luschnathii Schauer, A. pernambucensis Moldenke, A. verticillata Vell. e Amasonia campestris (Aubl.) Moldenke) e cinco à subfamília Viticoideae (Vitex gardneriana Schauer, V. megapotamica (Spreng.) Moldenke, V. orinocensis Kunth., V. rufescens A. Juss. e V. schaueriana Moldenke). O tratamento taxonômico inclui uma chave para identificação dos gêneros e das espécies, descrições, ilustrações, comentários taxonômicos, distribuição geográfica, hábitats e dados fenológicos das espécies.
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A chronological review of botanical explorations in Colombian was made through the revision of notes and publications from early 1500 ́s to late 1990 ́s. Complementary, herbaria photographs of Lamiaceae collections from botanical explorations in Colombia, deposited in the New York Botanical Garden Herbarium were analyzed in order to have a clear idea about the researches that were involved with collections in Colombia during the last 150 years.
A través de la consulta a notas científicas y publicaciones de varios autores fue hecha una revisión de la historia botánica de Colombia desde los años de 1500 ́s hasta 1990 ́s. Complementariamente, fueron analizadas fotografías de ejemplares de herbario de las colecciones de LamiaceAE provenientes de Colombia, depositadas en el Herbario del Jardin Botanico de Nueva York. Las especies, nombres de los colectores y sus instituciones de afiliación fueron comparadas y son presentadas seguidas de una breve discusión relacionada con el legado botánico de Colombia.
Subject(s)
History, 16th Century , History, 17th Century , History, 18th Century , History, 19th Century , History, 20th Century , Botany/history , Expeditions , Lamiaceae , ColombiaABSTRACT
Three new lignan glucosides, baicalensinosides A-C (1-3), were isolated from the roots of Scutellaria baicalensis. The structural elucidation was achieved by in-depth spectroscopic examinations and qualitative chemical test. Structurally, these compounds belong to the 3,4-dibenzyltetrahydrofuran-type lignan glycoside with a mono-hydroxyl substitution at the 7'-position of benzylidene group on the numbering system of lignans being one of their shared critical features. The anti-osteoporotic activity of the isolated compounds was assessed in an in vitro osteoprotegerin (OPG) transcriptional activity assay using dual luciferase reporter detection. At 10 μmol/L, compounds 1-3 increased the relative activating ratio of OPG transcription to 1.83, 0.84 and 0.98 times that of the control group, respectively.
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Este estudo avaliou os índices produtivos de matrizes suínas alimentadas com dietas contendo uma mescla líquida de óleos essenciais. Foram utilizadas 250 matrizes de ordens de parto (OP) entre um a oito, com duas dietas experimentais na gestação e duas na lactação (dietas controle e com 200ppm de óleos essenciais). O delineamento experimental foi o de blocos ao acaso, em um arranjo fatorial 2x2 (tratamentos na gestação e na lactação) com as matrizes e leitegadas como unidade experimental. O peso vivo médio da leitegada ao desmame e o número de desmamados foram 8,0% e 3,8% superiores (P<0,05), respectivamente, para os leitões filhos de matrizes suplementadas com óleos essenciais. A temperatura corporal das fêmeas do controle e OP cinco, avaliadas no período pré-parto e durante o parto, foi 4,4% e 3,5% superiores (P<0,05) aos óleos essenciais, respectivamente. A adição de óleos essenciais na dieta de matrizes no final de gestação e no início da lactação aumentou o número de leitões desmamados e o peso de leitegada. As fêmeas suplementadas com óleos essenciais não apresentaram temperatura e sinais clínicos condizentes com disgalactia.
The study evaluated the production index of sows fed diets containing a blend of essential oils. A total of 250 sows of parturition orders (PO) between one to eight, with two experimental diets during gestation and in the lactation (control diet with 200ppm and essential oils). The experimental design was a randomized blocks in a 2x2 factorial arrangement (treatments during pregnancy and lactation) with the sows and piglets as experimental unit. The average live weight of piglets at weaning and number of weaned were 8.0% and 3.8% higher (P<0.05), respectively for piglets from mothers supplemented with essential oils. The body temperature of females of the control and PO five, evaluated in the pre-parturition and in the parturition, was 4.4% and 3.5% higher (P<0.05) to essential oils, respectively. The addition of essential oils in the diet of sows in late pregnancy and early lactation increased the number of piglets weaned and litter weight. Females supplemented with essential oils, did not show temperature and clinical signs consistent with disgalactia.
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<p><b>OBJECTIVE</b>To evaluate antioxidant, antimicrobial and cytotoxic activity of different parts (root, flower, leaf and stem) of Leucas aspera (L. aspera) (Labiatae).</p><p><b>METHODS</b>Different parts of L. aspera were extracted with 80% (v/v) methanol. The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay.</p><p><b>RESULTS</b>All the extracts showed moderate to potent antioxidant activity, among which the root extract demonstrated the strongest antioxidant activity with the IC50 value of 6.552 µg/mL. Methanol extract of root possessed antioxidant activity near the range of vitamin E and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of root, flower, leaf and stem showed notable antibacterial activity against tested microorganisms. The root extract showed the highest mean zone of inhibition ranging from 9.0-11.0 mm against tested microorganisms, at a concentration of 100 mg/mL. In the brine shrimp lethality bioassay, it was evident that the methanol root extract did not show significant toxicity. The LC50 value for 12 h and 24 h observation was 2.890 mg/mL and 1.417 mg/mL, respectively.</p><p><b>CONCLUSIONS</b>The present finding suggests that the methanol root extract of L. aspera could be developed as pharmaceutical products.</p>
Subject(s)
Animals , Anti-Bacterial Agents , Chemistry , Pharmacology , Toxicity , Antioxidants , Chemistry , Pharmacology , Toxicity , Artemia , Bacteria , Biphenyl Compounds , Metabolism , Lamiaceae , Chemistry , Methanol , Microbial Viability , Picrates , Metabolism , Plant Components, Aerial , Chemistry , Plant Extracts , Chemistry , Pharmacology , Toxicity , Plant Roots , ChemistryABSTRACT
Objective: To evaluate antioxidant, antimicrobial and cytotoxic activity of different parts (root, flower, leaf and stem) of Leucas aspera (L. aspera) (Labiatae). Methods: Different parts of L. aspera were extracted with 80% (v/v) methanol. The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay. Results: All the extracts showed moderate to potent antioxidant activity, among which the root extract demonstrated the strongest antioxidant activity with the IC50 value of 6.552 μg/mL. Methanol extract of root possessed antioxidant activity near the range of vitamin E and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of root, flower, leaf and stem showed notable antibacterial activity against tested microorganisms. The root extract showed the highest mean zone of inhibition ranging from 9.0-11.0 mm against tested microorganisms, at a concentration of 100 mg/mL. In the brine shrimp lethality bioassay, it was evident that the methanol root extract did not show significant toxicity. The LC50 value for 12 h and 24 h observation was 2.890 mg/mL and 1.417 mg/mL, respectively. Conclusions: The present finding suggests that the methanol root extract of L. aspera could be developed as pharmaceutical products.
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Introducción: Candida spp. y Aspergillus spp. son causa importante de infecciones a nivel mundial. Considerando la resistencia de estos patógenos a algunos de los antimicóticos disponibles, es necesaria la búsqueda de nuevos agentes antimicóticos. Diferentes aceites esenciales y extractos de plantas han mostrado actividad antimicótica in vitro. El objetivo de este estudio fue evaluar la actividad antimicótica, citotóxica y la composición química de aceites esenciales de la familia Labiatae. Materiales y métodos: Se evaluó la actividad antimicótica de 22 aceites de plantas de la familia Labiatae contra C. parapsilosis ATCC 22019, C. krusei ATCC 6258, A. flavus ATCC 204304 y A. fumigatus ATCC 204305, siguiendo las técnicas estándar EUCAST y CLSI M38-A para levaduras y hongos filamentosos, respectivamente. Adicionalmente la actividad citotóxica se evaluó en la línea celular Vero mediante la técnica colorimétrica del MTT. La caracterización de los aceites esenciales se llevó a cabo por cromatografía de gases acoplada a masas. Resultados: El aceite esencial mas activo fue el de Minthostachys mollis frente a todas las cepas evaluadas con rangos concentraciones mínimas inhibitorias (CMIs) entre 250 y 375 µg/mL. El aceite de la planta Hyptis mutabilis mostró actividad frente a A. fumigatus (CMI = 396.8 µg/mL). Estos aceites esenciales no fueron citotóxicos sobre las células Vero. Los componentes principales de los aceites de las plantas M. mollis y H. mutabillis fueron epóxido de cis-piperitona y 1,8-cineol, respectivamente. Conclusiones: Los aceites esenciales de las plantas M. mollis y H. mutabillis mostraron actividad antimicótica y no fueron citotóxicos en células Vero.
Introduction: Aspergillus spp. and Candida spp. are important cause of infections worldwide. Considering the resistance of these pathogens to some antifungal agents, there is greater need to search for new antifungal agents. Many extracts and essential oils isolated from plants have shown to exert antifungal effects in vitro. The aim of this study was to evaluate the antifungal, cytotoxic effect, and chemical composition of essential oils of family Labiatae. Materials and methods: Antifungal activity of twenty two essential oils from Labiatae family was evaluated against C. parapsilosis ATCC 22019, C. krusei ATCC 6258, A. flavus ATCC 204304 y A. fumigatus ATCC 204305, following EUCAST and M38-A standard protocols for yeast and filamentous fungi, respectively. Additionally, cytotoxic activity was evaluated on Vero cell line by colorimetric assay MTT. Essential oils was characterized by gas chromatography coupled to mass spectrometry. Results: The most active oil with all strains was obtained of Minthostachys mollis (MIC range 250 - 375 µg/mL). The essential oil from Hyptis mutabillis showed activity against A. fumigatus (GM-MIC = 396.8 µg/mL). These essential oils were not cytotoxic on Vero cells. The major components of essential oils from M. mollis and H. mutabillis were cis-piperitone epoxide and 1,8-cineol, respectively. Conclusions: Essential oils of H. mutabillis and M. mollis showed antifungal activity and they were not cytotoxic on Vero cells.
Subject(s)
Fungi , OilsABSTRACT
This is the first report about the antibacterial activity of Hyptis martiusii Benth. In this study the ethanol extract of H. martiusii was tested for its antimicrobial activity against strains of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The growth of all bacterial strains tested was inhibited by the extract. The diameter of inhibition zones varied from 13 to 20 mm for the extract. The MIC and MBC values ranged from 128 to > 1024mg/mL and 256 to > 1024 mg/mL, respectively. It is therefore suggested that extracts from H. martiusii could be used as an anti-Staphylococcus agent. Compared with methicillin and gentamicin, the extract was more effective, being a promising antibacterial agent.
Este é o primeiro relato de atividade antibacteriana de Hyptis martiusii Benth. Neste estudo, o extrato etanólico de H. martiusii foi avaliado para atividade antimicrobiana contra linhagens de Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus. O crescimento de todas as bactérias testadas foi inibido pelo extrato. O diâmetro das zonas de inibição variaram de 13 - 20 mm. Os valores da CIM e CBM variaram de 128 a > 1024 mg/mL e 256 a > 1024 mg/mL, respectivamente. Devido a isso, podemos indicar que o extrato etanólico de H. martiusii pode ser usado como um agente anti-Staphylococcus. Quando comparado com outros antibióticos como meticilina e gentamicina, o extrato foi mais efetivo, demonstrando ser um promissor agente antibacteriano.
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Neste estudo foi investigada a ação antimicrobiana e a inibição de aderência in vitro do extrato hidroalcoólico de Rosmarinus officinalis Linn. (alecrim) sobre cepas padrão de Streptococcus mitis ATCC 98811, Streptococcus sanguinis ATCC 10556, Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 27609 e Lactobacillus casei ATCC 7469. Os ensaios foram realizados pelas técnicas de ágar-difusão em placas de Petri para determinação da Concentração Inibitória Mínima (CIM) e técnica de tubos inclinados para determinação da Concentração Inibitória Mínima de Aderência (CIMA) ao vidro, na presença de 5 por cento de sacarose. Os mesmos procedimentos foram realizados com a clorexidina à 0,12 por cento. As CIMs (mg/mL) do extrato de Rosmarinus officinalis sobre S. sanguinis ATCC 10556, S. mutans ATCC 25175, S. sobrinus ATCC 27609 e L. casei ATCC 7469 foram 1:1, 1:4, 1:1 e 1:4, respectivamente. Não houve inibição de crescimento de S. mitis ATCC 98811. As CIMAs do extrato de Rosmarinus officinalis frente a S. mitis ATCC 98811, S. mutans ATCC 25175 e S. sobrinus ATCC foram 1:8, 1:16 e 1:8, nessa ordem. Os resultados sugerem a possibilidade de uso do extrato de alecrim como antimicrobiano oral. No entanto, modelos de estudo que possam reproduzir situações mais próximas àquelas encontradas na cavidade oral são requeridas para avaliação de agentes antimicrobianos no tratamento e prevenção de infecções orais biofilme-dependentes.
In this study was investigated the antimicrobial activity and in vitro adherence inhibition of a hydro alcoholic Rosmarinus officinalis Linn. (alecrim) on standard strains of Streptococcus mitis ATCC 98811, Streptococcus sanguinis ATCC 10556, Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 27609 and Lactobacillus casei ATCC 7469 extract. The test was carried out by inundation tecniques in Petri dishes to determine the Minimum Inhibitory Concentration (MIC) and inclined tubes techniques the Minimum Inhibitory Concentration of Adherence to glass (MICA) at the presence of 5 percent sucrose. Tests with gluconate of chlorexidine 0.12 percent were performed as controls. MICs of the Rosmarinus officinalis extract dilutions (mg/mL) against S. sanguinis ATCC 10556, S. mutans ATCC 25175, S. sobrinus ATCC 27609 and L. casei ATCC 7469 were 1:1, 1:4, 1:1 e 1:4, respectively. The extract from alecrim inhibited all the standard strains growth tested, except for S. mitis ATCC 98811. MICAs of the Rosmarinus officinalis extract dilutions (mg/mL) against S. mitis ATCC 98811, S. mutans ATCC 25175 e S. sobrinus ATCC were 1:8, 1:16 e 1:8, respectively. The results suggest that there is a possibility of the alecrim use as an oral antimicrobial. Nevertheless, study models which could reproduce situations similar to those seen in bucal caries are necessary for the antimicrobial agents evaluation in the treatment and biofilm dependant oral infections prevention.
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Essential oils present antimicrobial activity against a variety of bacteria and yeasts, including species resistant to antibiotics and antifungicals. In this context, this work aims at the evaluation of the antimicrobial activity of the essential oil of Mentha x villosa Hudson ("hortelã da folha miúda"), its major component (rotundifolone) and four similar analogues of rotundifolone (limonene oxide, pulegone oxide, carvone epoxide and (+)-pulegone) against strain standards of Staphylococcus aureus ATCC 25923, E. coli ATCC 25922, Pseudomona aeruginosa ATCC 27853, Candida albicans ATCC 76645 and one strain of meticilin - resistant Staphylococcus aureus - MRSA (171c) from human clinic. The method of the diffusion in plates with solid medium was used. The results showed that the oil of Mentha x villosa, rotundifolone, limonene oxide and (+)-pulegone, are similar regarding the antimicrobial activity against the tested strains of S. aureus and C. albicans. All of the products present antimocrobial potential with antibacterial activity for S. aureus ATCC 25923 and antifungal activity for C. albicans ATCC 76645. None of the products presented antimicrobial activity for the strains of E. coli ATCC 25922 and P. aeruginosa ATCC 27853, representatives of the Gram negative bacteria.
Os óleos essenciais apresentam atividade antimicrobiana contra uma variedade de bactérias e fungos, incluindo espécies resistentes a antibióticos e antimicóticos. Neste contexto, este trabalho objetiva a avaliação da atividade de antimicrobiana do óleo essencial de Mentha x villosa Hudson (hortelã-da-folha-miúda) - seu componente majoritário (rotundifolona) e quatro análogos sintéticos da rotundifolona (epóxi-limoneno epóxi-pulegona, epóxi-carvona e (+)-pulegona) frente a cepas padrão de Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 76645 e uma cepa de Staphylococcus aureus meticilina - resistente - MRSA (171c) de clínica humana. Como método, foi utilizada a difusão em placas com médio sólido. Os resultados mostraram que o óleo de Mentha x villosa, rotundifolona, epóxi-limoneno e (+)-pulegona, são semelhante em função da atividade antimicrobiana para as cepas de S. aureus e C.albicans testadas. Todos os produtos apresentaram potencial antimicrobiano com atividade antibacteriana para S. aureus ATCC 25923 e atividade antifúngica para C. albicans ATCC 76645. Nenhum dos produtos apresentou atividade antimicrobiana para as cepas de E. coli ATCC 25922 e P. aeruginosa ATCC 27853, representantes das bactérias Gram negativas.
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Objective To study the chemical constituents of Ajuga decumbens. Methods Five compounds were isolated from methanol extraction and purified with silica gel column chromatography. Their structures were elucidated on the basis of spectral analysis. Results Five compounds were 1-octen-O-?-L-arabinopyranosyl-(1-6)-O-[?-D-glucopyranosyl-(1-2)]-?-D-glucopyranoside (Ⅰ), n-butyl-?-D-fructopyranoside (Ⅱ), 6, 7-dihydroxy-coumarin (Ⅲ), 5, 7-dihydroxy-4′-methylflavone (Ⅳ), and 3-O-?-D-glucopyranositosterol (Ⅴ). Conclusion 〖WT5BZ〗These five compounds are isolated from this plant for the first time.
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Foi verificada a presença do acido ursólico e seu derivado 2α-hidroxilado em Hyptis umbros. Salzmann, assim como do acido ursólico em Eriope crassipe. Bentham.
The presence of ursolic and its 2alfa-hydroxy derivative was established in the Labiatae Hyptis umbrosa Salzmann. Ursolic acid was also isolated from another Labiatae Eriope crassipes Bentham (Labiatae).