ABSTRACT
Objective To investigate the effect of mixed-skin grafting with autologous microskin and allogenetic acellular dermal matrix microskin on wound healing in rats,and to make a further study on the related mechanism.Methods Wistar rats were served as a allogenetic acellular dermal matrix donor rats,and SD rats as acceptors with mould of full thickness skin defects on their back.The ninety SD rats were divided into 5 groups with 18 rats in each group.Group 1 was transplanted with autologous microskin,and group 2 with allogenetic acellular dermal matrix microskin.Groups 3,4 and 5 were grafted with mixed-skin ratio between autologous microskin and allogenetic acellular dermal matrix microskin 1 ∶ 1,1 ∶ 0.5 and 1 ∶ 0.25,repectively.The rate of wound healing was measured,wound samples collected,hematoxylin and eosin stain carried out,fibronectin (FN) and laminin (LN)detected,and intergroup comparison made,respectively,2,3 and 4 weeks after skin grafting.Results The wound healing rates and FN and LN expression of mixed-skin grafting groups were higher than those of the group with autologous microskin grafting.The group of 1 ∶ 0.25 obviously increased (P＜0.05 or P＜0.01).Conclusions The wound healing rate with mixed-skin grafting is higher than that with autologous microskin grafting.The best effect is achieved when the skin ratio between autologous microskin and allogenetic acellular dermal matrix microskin is 1 ∶ 0.25.It is possibly due to the increase of FN and LN on wound skin surface.
ABSTRACT
Objective: To study the protective effect of Eucommia ulmoides polysaccharide (EUP) on liver-fibrosis rats and investigate its mechanism. Methods: Models of liver-fibrosis rats induced by carbon tetrachlotide (CCl 4) were established by sc injection of pure CCl4 (5 mL/kg) and then peanut oil with 40% CCl4 (3 mL/kg) to the back of rats for eight weeks. After the models were ig administrated by EUP for eight weeks, the indexes of the liver and spleen in liver-fibrosis rats were calculated; ALT and AST activities and contents of TP and ALB in serum were examined; Meanwhile, the ratio between ALB and GLOB (AJG) was computed; The four levels of HA, LN, PCIII, and IV-C in serum of liver-fibrosis rats were determined. SOD activities and Hyp, MDA, and GSH-Px levels in liver tissue were examined; The expression of transforming growth factor-β1 (TGF-β1) in liver was observed. Results: EUP could obviously be against the index increasing of the liver and spleen (P<0.01) in liver-fibrosis rats induced by CCl4; remarkably inhibit the increasing of ALT and AST activities in serum (P<0.01); decrease the content of HA, LN, PCIII, IV-C, and GLOB in serum (P<0.01); increase the content of TP and ALB, and ratio of A/G in serum (P<0.01); decrease the MDA amd Hyp levels in liver tissue (P<0.01); and improve SOD activities and GSH-Px levels in liver tissue (P<0.01); and decrease the expression of TGF-β1 as well. The best effect of EUP was observed in the high-dose group and inhibition of EUP on fibrosis was in a dose-dependent manner. Conclusion: EUP has the remarkable protective effects on liver-fibrosis rats.
ABSTRACT
Objective:To detect the contents of hyaluronic acid(HA),Laminin(LN) and probe their role in the pathologic course of chronic renal failure(CRF).Methods:The serum HA and LN from 37 patients with CRF and 40 healthy adults were measured by RIA.T test was used in data(x?s) analysis.Results:The HA and LN in CRF serum evidently increased than those of normal controls,respectively(P