ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the consequence of oral administration of Calliandra portoricensis (C. portoricensis) leaf extract on the stomach and pancreas in Swiss albino mice.</p><p><b>METHODS</b>Three groups of mice (B, C and D) were treated with 4 mg/kg of C. portoricensis extract. Group A was the control and received an equivalent volume of distilled water. Group B received C. portoricensis leaf extract for 7 days, Group C received C. portoricensis leaf extract for 14 days, and Group D received C. portoricensis leaf extract for 28 days. At different stages in the study, the mice were sacrificed and the stomach and pancreas were excised and fixed in 10% formol saline for histological analysis.</p><p><b>RESULTS</b>The result showed a normal microstructural outline in groups B and C as compared with the control. However, animals in group D showed disorganization of the mucosa and discontinuation of epithelial lining of the stomach while the islets of Langerans in the pancreas were at various degree of degeneration as compared with the control mice.</p><p><b>CONCLUSIONS</b>The present finding suggests that chronic administration (28 days as seen in this study) of C. portoricensis leaf extract may inhibit the proper function of the stomach and pancreas.</p>
Subject(s)
Animals , Mice , Fabaceae , Chemistry , Organ Size , Pancreas , Pathology , Plant Extracts , Pharmacology , Plant Leaves , Chemistry , Stomach , PathologyABSTRACT
Se reporta un inusual caso de granulomatosis de células de Langerhans localizado en maxilares en un paciente de 30 meses de edad, quien presentaba además de las lesiones líticas óseas, lesiones de aspecto tumoral en encía palatina y lingual de ambas arcadas dentarias. El examen óseo no arrojaba compromiso de otros huesos. El diagnóstico histológico fue corroborado por el Centro Nacional de Referencia en Anatomía Patológica.
A unusual case of Langerhans´ cells is reported, located in maxillae of a 30 months old patients, who, apart from bone lithic injuries, showed injuries of tumoral type in lingual and palatine gingiva of both dental archs. According to bone examination, there weren´t involvement of other bones. Histologic diagnosis was corroborated by National Reference Center in Pathologic Anatomy.
ABSTRACT
It has been shown that epidermal Langerhans cells (LCs) were antigen presenting cells in cutaneous cell-mediated immuneresponse,and they were implicated in skin allograft rejection,If LCs can be depleted from skin grafts,the survival period of the allografts may be prolonged. Therefore,we observed the changes of LCs in cultured human epidermal cells (ECs) by ATPase staining,HLA-Dr monoclonal antibody indirect immunofluoresence and enzyme labelling avidin-biotin complex (ABC) staining.And the capacity of ECs to stimulate allogeneic lymphocyte proligeration before and after cultures was testedby mixed epidermal cell-lymphocyte culture response (MEcLR).We found that LCs in original skin explants were significantly decreased and deformed three days after culture.LCs were reduced more than 90 percent after 7 days of culture.LCs in original skin explants were approximately eliminated 14 days or more of culture.LCs were never seen within epidermal outgrowth in culture.The Ecs gained from the original skin explants which were cultured after 7 days and from epidermal outgrowth lost their ability to stimulate allogeneic lymphocyte transformation in MEcLR.These results indicate that LCs bearing HLA-Dr antigen in human are able to be depleted from epidermal grafts by epidermal cell culture,and suggest that the ECs cultured in our culture system can prolong their survival period as they are used as allografts.