ABSTRACT
OBJECTIVE: To isolate and purify the reference compounds and improve the quality standard of Korean medicinal Herba Artemisiae sacrorum. METHODS: The chemical constituents were isolated from the aerial parts of Korean medicinal herb Artemisia sacrorum by silica gel, ODS column chromatography and preparative HPLC, and the structures were identified by NMR and MS. RESULTS: Fourteen known compounds were isolated and identified as follows:1α-acetoxyeudesm-4-en-6β,11βH-12,6-olide(1),(11S)-3-oxoeudesma-1,4(15)-dieno-12,6α-lactone(2), 1-epi-dehydroisoerivanin(3), 11-epi-taurin(4), chrysanthemolide(5), 1α-acetyl-gallicadiol(6), erivanin(7), 1α, 4α-dihydroxyeudesm-2-en-5α, 6β, 11βH-12, 6-olide(8), vulgarin(9),(+)-dehydrovomifoliol(10), isoevodionol(11),(+)-epi-pinoresinol(12), lariciresinol-4'-O-β-D-glucopyranoside(13), and lariciresinol-4-O-β-D-glucopyranoside(14). CONCLUSION: All compounds are obtained from this plant for the first time. These compounds can be used as reference substances for the quality control of this ethnic medicine.
ABSTRACT
Objective: To study the chemical constituents of ethyl acetate fraction from Patrinia scabra. Methods: Chemical constituents were isolated and purified by macroporous resin, silica gel, and Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of spectroscopic analysis and chemical evidence. Results: Twelve compounds were isolated and purified. Their structures were identified as 5-O-feruloylquinic acid (1), 7α-morroniside (2), 7β-morroniside (3), villosol (4), protocatechuic acid (5), β-daucosterol (6), isopatriscabroside I (7), patriscabroside I (8), patrinioside (9), pinoresinol-4,4'-di-O-β-D-glucopyranoside (10), lariciresinol-4'-O-β-D-glucopyranoside (11), and villosolside (12). Conclusion: Compounds 2-6 are isolated from this plant for the first time and the compound 1 is isolated from the plants of Patrinia Juss. (Valerianaceae) for the first time.
ABSTRACT
Objective: To study the chemical constituents in the antiviral fraction of Isatidis Radix and the activity of the monomer components. Methods: The water extract of Isatidis Radix was adsorbed by macroporous resin, and the antiviral fraction was eluted with 10% and 50% ethanol, repeatedly separated and purificated by silica gel column chromatography, reverse phase silica gel column chromatography, and Sephadex LH-20. The chemical structures were identificated using physicochemical constants and spectral data. Results: Ten compounds were isolated from the active fraction of water extract, and they were respectively identified as syringin (1), 4-(1, 2, 3-trihydroxypropyl)-2, 6-dimethoxyphenyl-1-O-β-D-glucopyranoside (2), isolariciresino (3), isolariciresinol 4-O-β-D-glucopyranoside (4), lariciresinol-4-O-β-D-glucopyranoside (5), lariciresinol-4, 4'-bis-O-β-D-glucopyranoside (6), 2-hydroxy-1, 4-benzenedi carboxylic acid (7), D-mannitol (8), indole-3-acetonitrile6-O-β-D-glucopyranoside (9), and 3-[2'-(5'-hydroxymethyl) furyl]-1(2H)-isoquinoline-7-O-β-D-glucopyranoside (10). Conclusion: Compounds 2, 4, 7, 8, and 10 are isolated from Isatidis Radix for the first time. Compound 10 has the significant effect of anti-HSV-2 virus. Therapeutic index (TI) is 6.07. Compounds 1, 5, and 6 also show some inhibition to HSV virus.
ABSTRACT
Objective: To study the chemical constituents from the rhizome of Drynaria fortunei. Methods: Silica gel, ODS, Sephadex LH-20 column chromatography, and semi-preparative HPLC were used to isolate pure compounds. The compounds were identified on the basis of their physicochemical properties and spectroscopic data. Results: Fourteen compounds were isolated from the rhizome of D. fortunei, including three lignans (1-3) and eight flavonoids (4-11). By spectroscopic techniques, such as 1H-NMR, 13C-NMR, and ESI-MS, these compounds were identified as (7′R,8′S)-dihydrodehydrodiconiferyl alcohol 4′-O-β-D-glucopyranoside (1), lariciresinol 4′-O-β-D-glucopyranoside (2), (-)-secoisolariciresinol 4-O-β-D-glucopyranoside (3), eriodictyol (4), eriodictyol 7-O-β-D-glucopyranoside (5), neoeriocitrin (6), naringin (7), luteolin (8), luteolin 7-O-β-D-glucopyranoside (9), luteolin 8-C-β-D- glucopyranoside (10), 2′,4′-dihydroxydihydrochalcone (11), maltol 3-O-β-D-glucopyranoside (12), β-sitosterol (13), and daucosterol (14). Conclusion: Compounds 1-3, and 11 are isolated from the plants in Polypodiaceae family for the first time, and compounds 5 and 8-10 are isolated from the plants of Drynaria (Bory) J. Sm. for the first time. Compound 1 is present as rotamers at room temperature.