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Objective Human papillomavirus (HPV) is presumed to be the cause of the development of head and neck squamous cell carcinoma.However,the current research results are not very sure.The article was designed to study the distribution of HPV infection in the tissues of laryngopharyngeal squamous cell carcinoma and laryngeal papilloma and its significance.Methods We collected samples of tissues from 67 patients who were treated in our hospital,including 41 cases of laryngeal papilloma,26 cases with laryngeal carcinoma,hypopharyngeal carcinoma,and oropharyngeal carcinoma.In the same period,20 cases of benign vocal cord polyps were treated as control group.PCR was applied in HPV typing test of fresh tumor tissues from operations,followed by the analysis on HPV infection type and distribution.Results In research group,44/67 cases was HPV-positive (65.7％),among which the HPV types of the laryngeal papilloma were HPVll (36/41) and HPV6 (4/41),and HPVI6 (6/26) in laryngeal carcinoma,hypopharyngeal carcinoma,and oropharyngeal carcinoma.While in control group,vocal cord polyps were HPV-negative without any multitype infection.Conclusion HPV16 may be a risk factor for the occurrence of laryngeal cancer and oropharyngeal cancer (non-alcoholic-induced or non-tobacco-induced cancer).HPV has high prevalence in Chinese laryngeal papilloma people,but HPV has low prevalence in Chinese laryngeal or hypopharyngeal carcinoma people.HPV testing of head and neck cancer is worth promoting.
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Objective Our study was aimed to study the clinical effect of carbon dioxide laser in the treatment of adult laryn -geal papilloma . Methods Clinical data of patients with adult laryngeal papilloma received treatment at our hospital from January 2012 to June 2014 was retrospectively analyzed .Patients were divided into two groups according to the type of treatment , observation group:received carbon dioxide laser treatment;control group:received tumor forceps treatment .Participants included 61 cases of pa-tients, the observation group of 26 cases, control group 35 cases.Compare the general clinical data , short-term clinical effects(opera-tion time, length of hospital stay , complications , short-term curative effect ) , the change of immune inflammation indexes before and after operation and postoperative recurrence in 1 year of two groups patients. Results The operation time and the length of hospital stay in observation group was significantly lower than that in control group[(33.66 ±4.71)min vs (37.19 ±5.21)min,(4.07 ±1.25)d vs (4.83 ±1.38)d, P 4.11) ng/L, (36.16 ±7.37) ng/L, (12.63 ±4.12) ng/L.The differences of these parameters between the two groups were signifi-cant (P<0.001).Observation group patients had a lower rate of recurrence 1 year after surgery than that of control group patients (12.00%vs 37.93%, P=0.029). Conclusion In the treatment of adult laryngeal papilloma , carbon dioxide laser can achieve a better minimally invasive and lower postoperative recurrence when compare to tumor forceps treatment .
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Objective. Describe factors associated with aggressive forms of recurrent respiratory papillomatosis (RRP). Materials and methods. One hundred eighty-nine RRP cases diagnosed between 1985 and 2009 were identified in pathological records. HPV was detected by the SPF-10 method with broad spectrum primers, (version 1). Results. 113 patients had only one surgery (less aggressive) and 76, two or more interventions (more aggressive). The likelihood of aggressive lesions decreased with increasing age at diagnosis and HPV-11 was associated with no significant increase in the risk of aggressiveness. Conclusions. The age at diagnosis was the main determinant of RRP aggressiveness.
Objetivo. Describir factores asociados con formas agresivas de papilomatosis respiratoria recurrente (PRR). Material y métodos. Se identificaron 189 casos de PRR diagnosticados entre 1985-2009 en registros patológicos. VPH fue detectado por el método SPF-10 con cebadores de amplio espectro, (versión 1). Resultados. 113 casos presentaron una intervención quirúrgica (menos agresivas) y 76, dos o más intervenciones (más agresivas). La probabilidad de lesiones agresivas disminuyó con el aumento de la edad al momento del diagnóstico y el VPH-11 se asoció con aumento no significativo del riesgo de agresividad. Conclusiones. La edad al momento del diagnóstico fue el principal determinante de la agresividad de PRR.
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Papillomaviridae/classification , Papillomavirus Infections/virology , Respiratory Tract Infections/virology , Colombia , Retrospective StudiesABSTRACT
AIM: To investigate correlation between the expression of survivin and caspase-3 proteins in juvenile laryngeal papilloma. METHODS: The expression of survivin and caspase-3 proteins were detected with immunohistochemial method in 43 cases of juvenile laryngeal papilloma, 25 vocal nodules and 25 normal laryngeal mucosa. RESULTS: The positive rates of survivin protein in juvenile laryngeal papilloma were 57.14% and higher than that in voeal nodules (P<0.01)and the normal laryngeal moeusa (P<0.01). And the Caspase-3 protein positive rate was 26.19% in juvenile laryngeal papilloma and lower than that in voeal nodules and the normal laryngeal mucosa (P<0.01).There was a significant negative correlation between the expression of survivin and caspase-3 in juvenile laryngeal papilloma. CONCLUSION: The abnormal expression of survivin and caspase-3 may play important role in the pathogenesis of juvenile laryngeal papilloma.
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Objective:To explore the relationship between the expression of EGFR and STAT3 in human la-ryngeal papilloma and its biological behavior. Method: Reverse transcription polymerasechain reaction(RT-PCR), immunohistochemical staining and Western blot were used to evaluate the mRNA and protein expression of EGFR and STAT3(p-STAT3) in 42 laryngeal papilloma tissues and 15 samples of normal laryngeal tissue, and the rela-tionship between the protein expression of them and clinicpathological parameters was also analysized. Result: The mRNA expression levels of EGFR and STAT3 in laryngeal papilloma tissue were significantly higher than that in normal laryngeal tissue(P<0.05,P<0.01). Protein positive expression of EGFR and p-STAT3 were also detected in a significantly greater proportion of laryngeal papilloma than normal laryngeal tissue by immunohistochemistry and western blot(P<0.01,P<0.05). There was relationship between EGFR and p-STAT3 overexpression in la-ryngeal papilloma(P<0.05). The expression p-STAT3 was correlated with the recurrence and canceration of la-ryngeal papilloma(P<0.05). Conclusion: The EGFR-STAT3 signal transduction pathway may be involved in the pathogenesis of laryngeal papilloma,, and the persistent activation of STAT3 gene plays an important role in the recurrence and canceration of laryngeal papilloma.
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BACKGROUND: Compared to condyloma acuminatum, the cause of laryngeal papilloma has not been clearly established. Although mode of viral transmission is not known yet, laryngeal papilloma seems to be caused by same viruses as condyloma acuminatum. OBJECTIVE: This study was done to investigate the HPV types of condyloma acuminatum and laryngeal papilloma and the epidemiologic relationship between these two diseases. METHODS: The polymerase chain reaction (PCR) was done in fifteen cases of condyloma acuminatum and ten cases of laryngeal papilloma. RESULTS: Same types of HPV DNA (HPV6,11) were detected in all cases of condyloma acuminatum and laryngeal papilloma. CONCLUSION: Laryngeal papilloma is considered a pure viral disease caused by HPV 6 or HPVll and closely related to condyloma acuminatum caused by HPV 6, 11.
Subject(s)
Humans , DNA , Human papillomavirus 6 , Papilloma , Polymerase Chain Reaction , Virus DiseasesABSTRACT
BACKGROUND: Compared to condyloma acuminatum, the cause of laryngeal papilloma has not been clearly established. Although mode of viral transmission is not known yet, laryngeal papilloma seems to be caused by same viruses as condyloma acuminatum. OBJECTIVE: This study was done to investigate the HPV types of condyloma acuminatum and laryngeal papilloma and the epidemiologic relationship between these two diseases. METHODS: The polymerase chain reaction (PCR) was done in fifteen cases of condyloma acuminatum and ten cases of laryngeal papilloma. RESULTS: Same types of HPV DNA (HPV6,11) were detected in all cases of condyloma acuminatum and laryngeal papilloma. CONCLUSION: Laryngeal papilloma is considered a pure viral disease caused by HPV 6 or HPVll and closely related to condyloma acuminatum caused by HPV 6, 11.