ABSTRACT
Objective: Based on the study of chemical composition research of Ilex latifolia, to develop an HPLC method for the simultaneous determination of latifoloside F, latifoloside H, latifoloside C, latifoloside D, kudinoside F and ilekudinoside E. The purity of the six saponins was confirmed by LC-MS. Methods: Chromatographic separation was performed on a Wonda Cract ODS-2 column (250 mm × 4.6 mm, 5 μm) with linear gradient elution of acetonitrile and water at a flow rate of 1 mL/min, and the detection wavelength was 210 nm. The mass spectrum was negative ion mode, the capillary temperature was 250℃, the solvent gas was nitrogen, the flow rate was 0.75 L/min, the spray voltage was 4.0 kV, and the lens voltage was 125V. Results: The six saponins were well separated and all calibration curves showed good linearity within the test ranges (r > 0.999 2). The average recoveries were between 99.52% and 100.96%, and RSD values were less than 2%. Conclusion: An accurate and simple method is first established for the qualitation and quantification of six saponins in I. latifolia. The results demonstrate that the method could be applied as a reliable quality control method for the seed of I. latifolia.