ABSTRACT
To establish a method for the identification of five species and one variety of medicinal plants from Diospyros, their leaf veins, epidermis, anatomic and powder characters were observed and compared with macro-morphological and microscopic methods. The results indicated the differences of secondary and tertiary veins among those Diospyros species. The single cell non-glandular hair and glandular hair exist in most species' epidermis while stone cells were only found in the leaf powders of two species. Through the study, the main differences of leaf macro- and micro-morphology of these species were obtained and practical keys were also established, which can provide scientific base not only for identification of these species during their vegetative stages, but also for accuracy authentication of the source of Kaki Folium.
ABSTRACT
The systematic analysis between 15 different species and one species with two accessions in Solanum has been carried out and need all over revisions and investigations. In continuation to our previous morphometric analysis, currently stomatal variation is analyzed using light microscopy and scanning electron microscopy. In addition to classical anomocytic and anisocytic stomatal types, new types of stomata (anisotricytic, paratetracytic, diacytic and paracytic) are noticed for the first time in the genus Solanum. The scanning electron microscopic study reveals the nature of stomatal pore, nature of peristomatal rims as well as the presence of epicuticular wax deposits and wax flakes which are of taxonomic significance. This study also indicates that foliar stomatal characteristics are valuable taxonomic traits, which can be utilized to address the taxonomic issues within the genus.
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<p><b>OBJECTIVE</b>To carry out a pharmacobotanical study of Lonchocarpus cyanescens (Schum & Thonn) Benth (L. cyanescens) and Leptoderris micrantha Dunn (L. micrantha) which are two key medicinal plants from the family Fabaceae.</p><p><b>METHODS</b>The epidermal peel was obtained by soaking the leaf in concentrated nitric acid (HNO3) in a petri dish. Both surfaces were carefully mounted on clean glass slides and dehydrated by ethyl alcohol, and stained with safaranin O for 2 min. Transverse sections of plant leaf were obtained by free hand sectioning. Phytochemical screening for various constituents was carried out on the powdered leaves. Other parameters such as, moisture content, ash value, acid insoluble ash, water-soluble ash, water and alcohol extractive values were obtained by standard techniques.</p><p><b>RESULTS</b>THE DISTINCTIVE FEATURES OF THE SPECIES INCLUDE: the presence of stomata on both surfaces of L. cyanescens and the absence in L. micrantha. Presence of larger epidermal cells in both upper and lower surfaces of L. cyanescens [(35.25±1.64)×(31.25±2.36), (43.0±2.63)×(39.5±5.11)] respectively compared to L. micrantha. Glandular multicellular trichomes are present in L. micrantha but absent in L. cyanescens. Numerous trichomes surround the transverse section of the leaf of L. micrantha but absent in L. cyanescens. Preliminary phytochemical screening showed that both species contain secondary metabolites such as alkaloids, anthraquinones, cardiac glycosides, tannins, saponins, steroids and flavonoids.</p><p><b>CONCLUSIONS</b>The microscopic and phytochemical data provided in this study are useful for the standardization of the medicinal plants.</p>
ABSTRACT
Object To study the micro morphological characteristics of the leaf and stem epidermis of three species in Rabdosia (Bl ) Hassk Methods The leaf and stem epidermis of Rabdosia amethystoides (Benth ) Hara, R macrocalyx (Dann) Hara and R nervosa (Hemsl ) C Y Wu et H W Li were observed by scanning electron microscope (SEM) Results Some characteristics are same among three species, such as elliptical stoma and glandular scale which has four cells, but some characteristics are di fferent, such as the shape of epidermal cell, the distribution of nonglandular hair, the shape of stoma Conclusion The leaf and stem epidermal characteristics are obviously different among the three species, it provides the new reference to discriminate the plants of Rabdosia (Bl ) Hassk
ABSTRACT
Objective To explore an approach to identifying the plant source of Flos Lonicerae and Lonicera confusa rapidly, precisely, and efficiently. Methods Using LM and SEM to observe and compare the characters of leaf epidermis of the five species of Lonicer L. including L. japonica, L. confuse, L. hypoglauca, L. dasystyla, and L. macrantha. Results There are obvious differences in leaf epidermis among the five species of Lonicera L. under LM and SEM. Conclusion The characteristics of leaf epidermis can be used to identify the plant source of Flos Lonicerae and L. confusa. According to their characteristics under LM and SEM, the retrieval keys to the five species of Lonicera L. have been compiled.