Scavenger Receptor Class A to E Involved in Various Cancers / 전남의대학술지

Scavenger receptors typically bind to multiple ligands on a cell surface, including endogenous and modified host-derived molecules and microbial pathogens. They promote the elimination of degraded or harmful substances such as non-self or altered-self targets through endocytosis, phagocytosis, and adhesion. Currently, scavenger receptors are subdivided into eight classes based on several variations in their sequences due to alternative splicing. Since recent studies indicate targeting scavenger receptors has been involved in cancer prognosis and carcinogenesis, we will focus on the current knowledge about the emerging role of scavenger receptor classes A to E in cancer progression.

Lectin-Like Oxidized LDL Receptor 1 Mediates the Uptake of the C-Terminal Domain of Hsp70 (A Promising Immune Adjuvant Molecule) and Antigen Peptide Complexes.

Extracellular heat shock protein 70 (Hsp70) is an adjuvant molecule that stimulates the immune system. The C-terminal domain of Hsp70 (C70), without the ATPase domain, is sufficient for antigen cross-presentation. However, the mechanism by which the receptor mediates the uptake of C70–peptide complex remains unclear. We therefore aimed to determine the process by which the receptor mediates the uptake of antigenic peptide-bound C70. Methodology: Hsp70 and C70 individually cloned into pET28a were expressed in Escherichia coli BL21 (DE3) and were purified on Ni-NTA agarose and MonoQ HR5/5. Hsp70 and C70 were labeled with Alexa 555 and Alexa 633, respectively, to detect cellular binding. HEK293 cells stably expressing lectin-like oxidized LDL receptor-1 (LOX 1) and KG-1 human dendritic-like cells were incubated with Alexa-labeled Hsp70 and C70 individually or with C70 and antigenic complexes and were observed using fluorescence microscopy. The affinity of LOX-1 toward Hsp70 and C70 was analyzed by chip assay using surface plasmon resonance, which immobilized LOX-1 ligand recognition domain. Results: HEK293 cells stably expressing LOX-1 and KG-1 cells accepted the C70– peptide and Hsp70–peptide complexes. Anti-LOX-1-neutralizing antibody inhibited the uptake of the C70–peptide complexes by KG-1 cells. The dissociation constant (KD) of C70 toward the LOX-1 extracellular domain, measured by surface plasmon resonance, was 4.02 × 10−7 M and that of the C70–peptide complex was 6.6 × 10−8 M. C70 increased the LOX-1 affinity by forming a complex with the antigen peptide. Conclusion: Our findings suggest that LOX-1 is the primary receptor for the C70–peptide and the Hsp70–peptide complexes. C70 is a promising adjuvant molecule that is internalized via LOX-1. In addition, it is convenient to prepare C70 using an E. coli expression system and C70 is more stable than full-length Hsp70.

Possible Implications of Placental Expressions for Heat Shock Protein 70 and Lectin-like Oxidized Low Density Lipoprotein Receptor-1 on Postnatal Blood Pressure in Their Newborns / 체질인류학회지

The 'fetal origin' hypothesis propose the alteration in fetal environment result in developmental adaptation, the permanently change in structure, physiology and metabolism, thereby predisposing to cardiovascular, metabolic and endocrine disease in adult life. Evidence is accumulating that the fetal environment affects newborn cardiac structure and function in humans, and blood pressure (BP) in newborn predicts the likelihood of developing hypertension in adult life. However, few studies have reported the influence of fetal factors on BP in neonates and an attempt to relate fetal factors to a neonate's BP seems to be important to identify individuals at risk of developing hypertension later in life. As the placenta is the regulator of nutrient composition and supply from mother to fetus and the source of hormonal signals that affect maternal and fetal metabolism, appropriate development of the placenta is crucial to normal fetal development. By virtue of these roles the placenta is in a key position to play a direct role in fetal programming. The aim of this study was to evaluate positive relationship between placental oxidative stress and BP in their healthy newborn offsprings, and propose to relate fetal factors to a neonatal BP. Systemic blood pressure was measured by automated device in 68 healthy term newborns who were born at Ewha Womans Medical Center, and their tissue samples of placentas were obtained from 40 cases which are 20 cases from high neonatal blood pressure group and 20 cases from low neonatal blood pressure group. We investigated placental expressions for heat shock protein (HSP) 70 and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1), as markers for placental oxidative stress using immunohistochemistry and Western blot analysis, and evaluated their association with BP in healthy term newborn babies. The mean values of placental LOX-1 and HSP 70 were significantly higher in newborns with high BP group compared to those with low BP group. Increase in placental oxidative stress was associated with higher newborn systolic blood pressure. These findings suggest that newborn blood pressure may represent prenatal influence on cardiac structure and function.

Captopril suppresses lectin-like oxidized LDL receptor-1 protein in rats after acute lung injury / 第三军医大学学报

Objective To study the expression of lectin-like oxidized LDL receptor-1 (LOX-1) protein in acute lung injury (ALI) and the effect of captopril on this expression in order to investigate its role in the process and preliminary intervention.Methods Sprague-Dawley rats were randomly divided into 3 groups,normal control group,lipopolysaccharide (LPS) groups,and a LPS+captopril group,each group 10 rats.ALI model was induced by intratracheal injection of LPS (5 mg/kg),then those of LPS+captopril group were give an intraperitoneal injection of captopril (1.25 mg/kg).The animals of normal control group received an intratracheal injection of normal saline.In 6 h after LPS injection,the level of p(O2),wet/dry ratio (W/D),concentration of total protein in bronchoalveolar lavage fluid (BALF) and lung tissue histopathological changes were examined.The expression of LOX-1 protein in the lung tissue was measured by Western blot analysis.HE staining was used to examine the pathological changes of the lung.Results Histological examination showed that extensive lung inflammation were seen in the LPS group,which manifested by accumulation of significant numbers of neutrophils.The level of p(O2) in LPS group [(6.86?0.75) kPa] was decreased compared with that in sham group [(12.14?0.60) kPa,P