Niveles de urea en leche en sistemas silvopastoriles / Urea Levels in the Milk in silvopastoral Systems

Resumen El exceso de proteína en la dieta puede generar problemas productivos y reproductivos en rumiantes. La medición de la urea en la leche permite determinar la disponibilidad ruminal de energía y proteína, y, así, hacer ajustes en la dieta de ser necesario. El objetivo de este estudio fue cuantificar la concentración de urea en la leche en un hato que pastorea sistemas silvopastoriles multiestrato en alta densidad. Se evaluó un sistema doble propósito en El Cerrito, Valle del Cauca (Colombia). Los animales estuvieron divididos en tres grupos de alimentación (praderas con guinea-leucaena-algarrobo, estrella-leucaena-algarrobo, y estrella-algarrobo, respectivamente). Se recolectaron muestras de leche en el ordeño de la mañana y la tarde de cada animal seleccionado en cada grupo, a las cuales se les determinó la concentración de urea (Ureasa/ Berthelot). La concentración de urea en la leche del ordeño de la mañana y la tarde estuvo correlacionada positivamente (r = 0,86), la concentración promedio fue de 22,7±9,4 mg/dl. Sin embargo, entre grupos de alimentación varió (p < 0,001), fue mayor en el primer muestreo (p < 0,001), y también se observó una amplia variación entre los muestreos de una misma base forrajera. Los sistemas silvopastoriles multiestrato presentan adecuado balance entre la materia orgánica fermentable y la proteína degradable a nivel ruminal.

Abstract Excess of protein in the diet can lead to both productive and reproductive problems in ruminants. Measuring the urea in the milk allows determining the ruminant availability of energy and protein and, therefore, to do some adjustments to the diet whenever necessary. This study aims to quantify the urea concentration in the milk in a farm that takes care of high-density multi-layer silvopastoral systems. A system with dual purpose was evaluated in El Cerrito, Valle del Cauca (Colombia). The animals were divided into three feeding groups (prairies with guinea-leucaena-algarrobo, estrella-leucaena-algarrobo and estrella-algarrobo, respectively). Samples of milk were gathered during the morning and afternoon milking from the animal selected in each group, and then the urea concentration was determined (Urease/ Berthelot). The urea concentrations in the morning and afternoon milk were correlated positively (r = 0.86), the average concentration was 22.7+9.4 mg/dl. However, the values varied between the feeding groups (p < 0.001), it was higher in the first sample (p < 0.001). Likewise, a broad variation was observed between the samples inside the same foraging basis. Multi-layer silvopastoral systems show an appropriate balance between the fermentable organic matter and the degradable protein in the ruminants.

Desenvolvimento de mudas de Mimosa caesalpiniifolia Benth e Amburana cearensis (Fr. All) A.C Smith. irrigadas com esgoto doméstico tratado / Development of seedlings of Mimosa caesalpiniifolia Benth e Amburana cearensis (Fr. All) A.C Smith. irrigated with treated domestic wastewater

RESUMO Avaliaram-se os efeitos da aplicação de diferentes diluições de um esgoto doméstico tratado como água de reúso no desenvolvimento de mudas de Mimosa caesalpiniifolia Benth e Amburana cearensis (Fr. All) A.C Smith. O experimento se deu por meio da utilização do arisco como substrato e da irrigação com os tratamentos compostos por cinco diluições de água de reúso com água de abastecimento, com cinco repetições cada, sendo eles: T1 (100% água de reúso + 0% água de abastecimento), T2 (75% água de reúso + 25% água de abastecimento), T3 (50% água de reúso + 50% água de abastecimento), T4 (25% água de reúso + 75% água de abastecimento) e T5 (0% água de reúso + 100% água de abastecimento). Os efeitos das diferentes diluições do esgoto tratado foram avaliados nas seguintes características funcionais: número de folhas, tamanho da raiz principal, quantidade de matéria seca das folhas, da raiz principal e das raízes secundárias. Além disso, foram mensurados altura da planta e diâmetro do coleto durante 30, 60 e 90 dias. Os resultados obtidos para A. cearensis demonstraram que matéria seca das folhas e das raízes secundárias diferiram entre os tratamentos, sendo encontrados os maiores valores em T2, T3 e T4. Nos dados de diâmetro, ao longo do tempo ocorreu diferença significativa aos 90 dias para o tratamento T3. Para M. caesalpiniifolia, as diluições do esgoto não apresentaram diferença significativa entre os tratamentos. Desse modo, concluiu-se que o esgoto doméstico tratado proporcionou efeito positivo ou neutro no desenvolvimento, podendo ser uma fonte hídrica, na forma diluída ou concentrada, potencial para a irrigação de mudas dessas espécies.

ABSTRACT This work has assessed the effects of different dilutions of treated domestic wastewater, such as reuse water, on the development of Mimosa caesalpiniifolia Benth and Amburana cearensis (Fr. All) A.C Smith. seedlings. The experiment made use of sandy soil as substrate and irrigation with treatment consisted of five dilutions of reuse water and public supply water (PSW), with five repetitions each, as follows: T1 (100% reuse water + 0% PSW), T2 (75% reuse water + 25% public supply water), T3 (50% reuse water + 50% public supply water), T4 (25% reuse water + 75% public supply water), and T5 (0% reuse water + 100% public supply water). Effects of the different dilutions of treated wastewater were evaluated according to the following functional characteristics: number of leaves, size of primary root, dry matter content of leaves, dry matter content of primary root, and dry matter content of lateral roots. In addition, plant height and collar diameter were also measured over the period of 30, 60, and 90 days. Results obtained for A. cearensis show that dry matter content of leaves and dry matter content of primary root had the highest values with T2, T3, and T4. As far as diameter over time is concerned, there was a significant difference for the T3 treatment in 90 days. Treatments did not produce any meaningful differences in the case of M. Caesalpiniifolia. It was possible to conclude, therefore, that treated domestic wastewater had neutral or positive effects on development and could be a potential water source to irrigate seedlings of these species, in both diluted and concentrated forms.

Structural Characterisation of the Leaf of Bauhinia monandra Kurz (Fabaceae - Cercidoideae)

Abstract Bauhinia monandra Kurz (Fabaceae) is an ornamental leguminous tree from Africa. Its leaf has been proven to be hypoglycaemic, and are a source of lecithin BmoLL, providing this species with great medicinal potential. Curiously, there are no reports of the anatomical description of the leaf of this species. The present work aimed at describing the leaf anatomy of B. monandra, and make histochemical inferences. To this end, anatomical procedures in light and scanning electron microscopy were performed from different parts of the leaves of plants growing in the state of Bahia, Brazil. The leaf is amphistomatous. It possesses papillate epidermis, nonglandular and navicular trichomes, and the mesophyll is dorsiventral. The central vein is distinct from the others of the first degree. The first, second, and third-degree veins possess thick fibre bundles with associated monocrystals. The other veins are a little fibrous and excessively reduced. The petiole vascularisation consists of circularly arranged collateral bundles, plus two accessory bundles. The pulvinus have a wide parenchymatic cortex with abundant druses and vascularisation restricted to the central region, surrounded by a crystalline sheath and by a starch sheath. The motile cushion has the vascular bundles arranged in series. Associated with the phloem from the pulvinus vascular bundles and the motile apparatus, occur non-lignified septate fibres that confer flexibility to these organs. The absence of lateral projections and the arrangement of vascular bundles in the petiole was the most distinctive anatomical features and of the greatest taxonomic potential observed in the leaf of B. monandra.

Obtención de hidrolizados proteicos de leguminosas usando una proteasa recombinante de Pseudomonas aeruginosa M211 / Obtaining protein hydrolysates from leguminous using a recombinant protease from Pseudomonas aeruginosa M211

El género Pseudomonas es una fuente importante de proteasas; sin embargo, su uso está restringido en la industria alimentaria. El clonaje permite aprovechar la capacidad catalítica de estas enzimas mediante su producción en microorganismos inocuos. Por otro lado, las leguminosas son fuentes ricas en proteínas, a partir de las cuales se pueden obtener compuestos con valor agregado mediante procesos de hidrólisis enzimática. En este estudio, se produjo y caracterizó una proteasa recombinante (PT4) alcalina y termoestable de Pseudomonas aeruginosa M211, para la obtención de hidrolizados proteicos de leguminosas. Para ello, el gen de la proteasa se clonó en el vector pJET1.2/blunt utilizando E. coli DHalfa como hospedero. El análisis de la secuencia nucleotídica parcial de la proteasa indicó un 99 % de similitud con Peptidasas de la Familia M4 de Pseudomonas aeruginosa. La enzima recombinante presentó un peso molecular de 80 kDa, demostró ser activa y estable en condiciones alcalinas y termófilas con un pH y temperatura óptimos de 8 y 60 °C, respectivamente, y fue inhibida por EDTA. Además, hidrolizó proteínas de semillas de Glycine max, Phaseolus lunatus, Lupinus mutabilis y Erythrina edulis, obteniéndose fracciones peptídicas menores a 40 kDa. Esta proteasa recombinante se podría utilizar en la elaboración de hidrolizados proteicos funcionales a partir proteínas de distintas fuentes y residuos agroalimentarios.

The genus Pseudomonas is an important source of proteases; however, in the food industry the use of this bacterium is restricted. Cloning allows for the use of the proteolytic activity of Pseudomonas proteases through their production in innocuous microorganisms. Leguminous are protein-rich sources from which value-added compounds can be obtained through enzymatic hydrolysis. In this study, an alkaline and thermostable recombinant protease (PT4) from Pseudomonas aeruginosa M211 was cloned and characterized in order to obtain protein hydrolysates from leguminous. Therefore, protease gene was cloned into the pJET1.2 / blunt vector using E. coli DHalpha as a host. Analysis of protease partial nucleotide sequence showed 99% homology with Peptidases M4 Family from Pseudomonas aeruginosa. The molecular weight of the recombinant enzyme was 80 kDa, it was active and stable under alkaline and thermophilic conditions, presented an optimum pH and temperature of 8 and 60 °C, respectively, and was inhibited by EDTA. In addition, it hydrolysed Glycine max, Phaseolus lunatus, Lupinus mutabilis y Erythrina edulis proteins, obtaining peptide fractions less than 40 kDa. This recombinant protease could be used in the elaboration of functional hydrolysates using protein from different sources and agricultural waste.

Consequence of chromium-tainted soil on physical and biochemical responses of Vigna radiata L.

Mung bean (Vigna radiata L.) is one of the important leguminous plants of India, with shorter growing season. In this work, the intention was to correlate the effects of chromium on physical and biochemical responses of V. radiata L. Mung bean seeds were germinated and grown under controlled treatment, with different concentrations of waste soil mixed with garden soil. Physical parameters such as shoot length, root length, and fresh weight were found highest in control soil and 25% of contaminated soil, whereas 50% and pure contaminated soil showed poor growth stage and poor quality physical parameters. Biochemical parameters such as total chlorophyll content, protein content, and starch content were found highest in control soil and 25% contaminated soil, but these parameters were found to be less in 50% and pure contaminated soil. It was observed that physical and chemical parameters were declining with increasing chromium contamination.

Inhibitory Effect of Rhizobia Isolated from Several Leguminous against Phytopathogenic Fungi

Aims: This study was carried out to evaluate the antagonistic effect of ten isolated characterized Rhizobium sp. and three referenced strains against aggressive phytopathogenic fungi Fusarium spp., Aspergillus spp., Penicillium spp., Alternaria spp., Humicola spp. and Cladosporium spp. isolated from infected and wilted plants in vitro and in vivo. Study Design:  First, we have ten strains of rhizobia isolated from leguminous and characterized, after that,  some strains of fungi were isolated from infected  and wilt plants,  such as Fusarium, Aspergillus, Penicillium,  Alternaria, Humicola and Cladosporium. Finally, The investigation of the potential of the isolated rhizobia and three referenced strains was evaluated in dual culture, in pots experiments and on seeds. Place and Duration of Study: The study was carried out at the Department of Microbiology, Faculty of Nature and Life Sciences, Laboratory of Applied Microbiology, between April and November 2016. Methodology: Rhizobia isolates were identified upon their phenotypic traits as: the morphology of the colony, of the physiological characters (growth temperature, salt tolerance, resistance to antibiotics, metabolism of carbon source, generation time…) and also the production of extracellular metabolites as siderophores and proteases. Fungal isolates were identified by their macroscopic and microscopic characters. The antagonistic effect of rhizobia against phytopathogenic fungi was examined in vitro by: confrontation in Petri dishes and inoculation of seeds; and in vivo by inoculation of plants. Results: The isolated rhizobia were resistant to penicillin and amoxicillin with generation time ranged from 1.9 to 6.4 h, they were able to grow at temperatures from 4°C to 37°C, tolerate salt concentration (0.5 –2%) and produce siderophores and proteases. The results demonstrated the effectiveness of the rhizobia isolates and the referenced strains against the fungi isolates in vitro and in vivo. In dual culture, all rhizobia isolates inhibited the mycelial growth of the pathogens. The best disease control was obtained with isolate F3 from faba bean which inhibited the fungal growth with more than 70%. The highest inhibition growth was obtained against Alternaria spp.2, Penicillium spp.2, Cladosporium spp. and Humicola spp. with an inhibition rate ranging from 90 to 96%. In pot experiments, Rhizobia isolates from chickpea, lentil and faba bean reduced significantly all disease incidence  with more than 75%; where the best fight was observed in lentil plants, while in faba bean no wilted plants were observed. Seeds inoculated with rhizobia and fungi in Petri dishes showed that isolates from faba bean and lentil were the most effective in reducing fungi disease incidence. Conclusion: Rhizobia have a high potentiality to inhibit the growth of tested pathogens and could be fielded within an integrated disease management package.

Study of Some Pucciniales Encountered on Leguminous Plants in Morocco

This particular work deals with the Pucciniales fungi (Basidiomycetes) of Morocco. Surveys in northern Atlantic Morocco have allowed collecting dozen species of plants (Oxalis corniculata, Vicia sativa and Lotus edulis) infected by rust. The different symptoms that have been observed are described in the laboratory and a microscopic study of spores, allowed to identify the responsible pathogens for these symptoms which are like: Puccinia oxalidis, Uromyces ervi and Uromyces genistae-tincortoriae. The obtained results help to know the diversification of host Pucciniales and the description of other new species for that cause the fungal diversity of Morocco is to be known.

Dry matter yield and bromatological composition of gliricidia in different crop densities / Produção e composição bromatológica da massa seca de gliricídia em diferentes densidades de plantio

ABSTRACT: The objective of this study was to evaluate the effect of different gliricidia planting densities on productive and qualitative parameters. The experiment was carried out at the Experimental Station Pedro Arle, Embrapa Tabuleiros Costeiros (Embrapa Coastal Tablelands), in Frei Paulo, Sergipe, Brazil. The effect of densities of 10,000; 20,000; 30,000 and 40,000 plants ha-1 was tested in biomass production (fresh and dry), dry matter content (DM), crude protein (CP), neutral detergent fiber (NDF), and acid-detergent fiber (ADF), in gliricidia leaves and tender stems. The experiment consisted of a randomized block design with four replications. Production of leaf and stem fresh biomass; production of leaf dry matter; percentage of fresh leaves in relation to the total fresh matter; and percentage of leaf dry matter of 13 cuttings were evaluated from September 2nd, 2009 to December 18th, 2013. There was increase in the production of total fresh matter, leaf fresh matter, and leaf dry matter (P<0.05) in planting densities greater than 20,000 plants ha-1. Year effect (P<0.05) was found only for total production of fresh matter, production of fresh leaves, percentage of leaf fresh matter, leaf dry matter content and crude protein.

RESUMO: Este estudo teve como objetivo avaliar o efeito de diferentes densidades de plantio de gliricídia sobre parâmetros produtivos e qualitativos. O experimento foi realizado na Estação Experimental Pedro Arle, da Embrapa Tabuleiros Costeiros, em Frei Paulo, Sergipe, Brasil, o efeito das densidades de cultivo de 10.000; 20.000; 30.000 e 40.000 plantas ha-1 foi testado nos rendimentos de biomassa (verde e seca), teores de matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN) e fibra em detergente ácido (FDA) em folhas e caules mais tenros de gliricidia. Foi utilizado o delineamento em blocos casualizados com quatro repetições. A Produção de biomassa fresca de folhas e caules, a produção de massa seca de folhas, a porcentagem de folhas frescas relacionados à biomassa fresca total, a porcentagem de matéria seca nas folhas de 13 cortes foi avaliada durante o período de 02/09/2009 e 18/12/2013. As produções de biomassa fresca total, biomassa das folhas frescas, matéria seca de folhas aumentaram (P<0,05) com densidades de plantio superiores a 20.000 plantas ha-1. Foi encontrado efeito do ano (P<0,05) apenas para a produção total de biomassa fresca, produção de folhas frescas, porcentagem de biomassa de folhas frescas, teor de matéria seca das folhas e PB.

Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.

This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100 percent of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 = 0.43 to 26.19 = 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD50 > 0.15 = 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituentsfrom primary and secondary metabolism upon Ae. aegypti.

Este trabalho objetivou avaliar a toxicidade dos extratos aquosos de sementes de 15 espécies de leguminosas contra larvas de Aedes aegypti. Foi realizada uma caracterização química e bioquímica parcial dos extratos aquosos e a avaliação da toxicidade aguda em camundongos. Os extratos de Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum e Piptadenia moniliformis causaram 100 por cento de mortalidade depois de 1 a 3 h de exposição e mostraram valores de CL50 e CL90 entre 0,43 = 0,01 e 9,06 ± 0,12 e entre 0,71 = 0,02 e 13,03 = 0,15 mg/mL, respectivamente. Dentre os constituintes do metabolismo secundário, os extratos das sementes apresentaram taninos, fenóis, flavonas, flavonóis, xantonas, saponinas e alcalóides. Os extratos apresentaram alto teor de proteínas solúveis (0,98 to 7,71 mg/mL), lectina (32 to 256 UH/mL) e inibidor de tripsina (3,64 ± 0,43 to 26,19 = 0,05 gIT/kg de farinha). O perfil eletroforéticomostrou uma grande diversidade de proteínas, muitas dasquais já descritas como inseticidas. Os extratos mostraram baixa toxicidade ao camundongo (DL50 > 0,15 = 0,01 g/kg peso corporal), porém apesar desses resultados promissores, estudos posteriores são necessários para compreender a toxicidade desses extratos e de seus constituintes do metabolismo primário e secundário sobre Ae. aegypti.

Produção animal e valor nutritivo da forragem de pastagem de coastcross consorciada com amendoim forrageiro / Animal production and nutritive value of a coastcross pasture mixed with forage peanut

Neste estudo foram utilizados os tratamentos: coastcross + amendoim forrageiro + 200kg/ha de N; coastcross + amendoim forrageiro + 100kg/ha de N; coastcross + 200kg/ha de N e coastcross + amendoim forrageiro (parcelas) no inverno, primavera, verão e outono (sub parcelas), delineados em blocos ao acaso. Novilhas foram manejadas sob lotação contínua e taxa de lotação variável em pastagem mantida a 17cm de altura. Amostras foram coletadas a cada 28 dias para determinar o valor nutritivo da forragem. Foram avaliados: ganho médio diário (GMD), ganho de peso vivo (GPV), taxa de lotação (TL) e número de animais dia (NAD). Quanto ao valor nutritivo da forragem, os piores resultados ocorreram nas pastagens sem adubação, 16,9 por cento e 6,0 por cento de PB de folha e colmo, respectivamente, e 70,1 por cento de FDN de folha. Na primavera e no verão, o GMD foi mais alto, 0,518 e 0,515kg/animal do que no inverno e outono, 0,396 e 0,293kg/animal, respectivamente. A TL foi superior nas pastagens que receberam a maior dose de nitrogênio, 5,38UA/ha em média, e no verão, 6,81UA/ha. O GPV foi mais elevado nas áreas com adubação, 1341kg de PV/ha, em relação aos pastos não adubados, 735kg/ha.

In this study, the ollowing treatments were used: coastcross + forage peanut + 200kg/ha of N; coastcross + forage peanut + 100kg/ha of N; coastcross + 200kg/ha of N and coastcross + forage peanut (plots) in the winter, spring, summer, and autumn (subplots), designed in randomized blocks. Heifers were managed under continuous stocking and variable stocking rate on pasture maintained at 17cm height. Samples were collected every 28 days determining the nutritional value of forage. Average daily gain (ADG), weight gain (WG), stocking rate (SR), and number of animals/day (NAD) were evaluated. As for forage nutritional value, the worst results were found in pasture without fertilization, 16.9 percent and 6.0 percent CP of leaf and stem, respectively, and 70.1 percent NDF in leaves. In the spring and summer, animals had a higher ADG, 0.518 and 0.515kg/animal, than 0.396 and 0.293kg/animal in the winter and autumn, respectively. SR was higher in pastures that received higher nitrogen doses, 5.38AU/ha in average; and in the summer, 6.81AU/ha. LWG was higher in fertilized areas, 1,341kg LW/ha, than in not fertilized pastures, 735kg/ha.

Morpho-anatomical and micromorphometrical evaluations in soybean genotypes during water stress

In a greenhouse experiment, morpho-anatomical and micromorphometrical analyses of two soybean cultivars, MG/BR46 (Conquista) and BR16-tolerant and sensitive to drought, respectively-were used to study their water-deficit-tolerance strategies. Drought treatments were applied at reproductive stages from R2 to R7, where evaluations were conducted at 30 days and 45 days after stress started, respectively. The total length of Conquista plants (shoot + root) was greater than of BR16 plants. Pod dry weight was adversely affected due to the lack of moisture, decreasing productivity even of Conquista plants. Both the cultivars had normal development of root hairs; however, it was observed a decrease in the cortex:central cylinder ratio in BR16 stressed for 30 days, and they also showed similar leaflet thickness and stomata distribution. Differences in drought tolerance observed between the two cultivars seemed to be related to factors other than morphological traits since this species has a short lifecycle.

Análises morfo-anatômicas e micromorfométricas de duas cultivares de soja, MG/BR46 (Conquista) e BR16—tolerante e sensível à seca, respectivamente, em experimento conduzido em casa de vegetação—foram feitas para estudar as diferentes estratégias de tolerância ao déficit hídrico. Tratamentos de seca foram aplicados no estádio reprodutivo R2 e R7, onde avaliações foram conduzidas em 30 dias e 45 dias após o inicio do estresse, respectivamente. O comprimento total das plantas da cultivar Conquista (parte aérea e raiz) foi maior do que das plantas da cultivar BR16. A massa seca da vagem foi adversamente afetada pelo déficit hídrico, diminuindo a produtividade das plantas da cultivar Conquista. Ambas as cultivares tiveram o desenvolvimento de pêlos radiciais normais e, uma diminuição da razão córtex:cilindro central foi observada em BR16 estressada por 30 dias, além de apresentar uma espessura do folíolo e distribuição dos estômatos normais. Diferenças na tolerância à seca observada entre as duas cultivares devem estar relacionadas também a outros fatores, alm das características morfológicas, já que esta espécie possui um ciclo de vida curto.

Sustentabilidade de sistemas de rotação e sucessão de culturas em solos de várzea no Sul do Brasil / Crop rotations sustainability and successions systems in tilled plain areas in Southern Brazil

A avaliação quantitativa com diferentes tipos de indicadores é fundamental na determinação da sustentabilidade dos sistemas de rotação e sucessão de culturas. Este estudo teve como objetivo avaliar a sustentabilidade de algumas sucessões de culturas em solo de várzea, nos sistemas de plantio direto (PD) e convencional (SC). O trabalho foi conduzido em Pelotas, Rio Grande do Sul (RS), em área experimental da Embrapa Clima Temperado. Os tratamentos foram constituídos por cinco anos de implantação de sistemas de rotação e sucessão de culturas de inverno e de primavera-verão, seguidos de dois anos de pousio e três anos da cultura do arroz irrigado. As culturas de primavera-verão foram o milho e a soja, além do arroz; enquanto que as de inverno foram gramíneas, leguminosas, consórcios, nabo forrageiro e campo natural. O delineamento experimental foi o de blocos ao acaso com parcelas subdivididas, com três repetições. As culturas de verão ocuparam as parcelas, enquanto as espécies de inverno foram alocadas nas subparcelas. Os parâmetros observados foram transformados em índices para padronizar as diversas variáveis, cada uma em relação àquela de melhor comportamento mensurado, e dispostos em quatro categorias de análise: (a) indicadores agronômicos (matéria seca e rendimento de grãos); (b) indicadores ambientais (fertilidade do solo); (c) indicadores energéticos (produção e eficiência energética); d) indicadores econômicos (valor bruto da produção e rentabilidade). Pela média harmônica obtida entre os índices dos indicadores, foram comparados os diferentes sistemas de rotação e sucessão de culturas, inferindo-se daí sua sustentabilidade e suas diferenças (Teste t P≤0,05). Concluiu-se que: (a) todos os sistemas de rotação e sucessão de culturas dos quais o milho faz parte apresentam maior índice de sustentabilidade; (b) os sistemas de rotação e sucessão de culturas S1 [gramínea x soja x arroz (PD)] e S4 [nabo x soja x arroz (PD)] apresentam, ...

The quantitative analysis with different types of indicators is crucial in determining the sustainability of crop succession. This study aimed to appraise that in tilled plain areas. The research was carried out in Pelotas, RS, Brazil at Embrapa Clima Temperado research area. The treatments consisted of successions of winter and spring-summer crops species for five years, followed by two years of fallow and three years of rice cropping. The spring-summer crops were corn and soybean, besides rice; the winter crops were cereals, leguminous pastures, mixtures species, turnip and native grass species. Seeding was performed either by no-till (NT) or conventional (CT). The experiment was planned in a split-plot randomized blocks design with three replications, where the summer crops occupied the plots, while the winter's species were allocated to the subplots. The parameters measured were transformed into index to standardize the variables, each one in relation to the better measurement behavior, and arranged in four categories of analysis: (a) Agronomics indices (dry weight and grain yield); (b) Environmental indicators (soil fertility); (c) Energy Indicators (gain and energy efficiency); d) Economic Indicators (profitability and gross value of production). Through harmonic average rates calculated for each indicator, comparisons to the crop succession were then performed, and its sustainability inferred. Analyses of contrasts between the crop successions were accomplished too. The results provided the following conclusions: (a) All crop succession which had maize participation had a higher index sustainability; (b) the crops successions S1 [Cereals x soybean x rice(NT)] and S4 [Turnip x soybean x rice(NT)] had, respectively, the highest and the lowest sustainability among the ones with the soybean crop participation; (c) S5 [Native grass x soybean x rice(CT)] showed the lowest sustainability level, (d) S8 [Species mixtures x corn x rice(NT)] ...

Degradabilidade ruminal in situ de vagens de faveira (Parkia platycephala Benth.) em diferentes tamanhos de partículas / In situ ruminal degradability of faveira (Parkia platycephala Benth.) pods in different particle sizes

Estimaram-se os parâmetros de degradação ruminal da matéria seca (MS) e da proteína bruta (PB) de vagens de faveira, trituradas em partículas de 2 e 5mm, pelo método do saco de náilon in situ em ovinos, nos tempos de incubação 3, 6, 12, 24, 48, 72 e 96 horas, e determinou-se a degradabilidade efetiva, considerando-se taxas de passagem 2, 5 e 8 por cento/h. A fração a da MS e da PB foram 69,6 e 49,9 por cento, respectivamente, revelando elevada solubilidade da MS; a fração b para MS e PB foi 24,7 e 43,9 por cento, indicando baixa degradação da MS in situ, com estabilização da degradação da MS às 72h e da PB às 48h de incubação. O tanino de vagens de faveira não se mostrou depressor da degradabilidade in situ da PB

Soluble (a) and potentially degradable (b) fractions and degradation rate of b fraction (c) of dry matter (DM) and crude protein (CP) of Parkia platycephala pods in particle sizes 2 and 5mm were estimated by in situ nylon bag method in sheep. The times of incubation were 3, 6, 12, 24, 48, 72 and 96 hours, and the effective degradability (ED) was determined considering passage rates of 2, 5 and 8 percent/h. The a fractions for DM and CP was 69.6 and 49.9 percent, respectively, revealing elevated DM solubility: The b fractions for DM and CP was 24.7 and 43.9 percent, denoting reduced DM in situ degradation. The stabilization of the DM and CP degradation occurred at 72h and 48h after incubation, respectively. In situ degradability of constituents of P. platycephala pods, in special CP, were not depressed for their tannin contents