ABSTRACT
Objective To select the best condition of preparation process for the water-extraction and alcohol-precipitation method of Radix astragali in “Leifengguan” granules. Methods The effective compound astragaloside IV of Radix astragali was determined with the methods of TLC-scanning. The preparation process was screened with orthogonal design [L9(34)] and single factor analysis. Results The best condition of preparation process for water-extraction of Radix astragali was A3B2C2 that the drug materials were decocted for 3 times with 8 times amount of water, each time for 1 hour. After the solution was concentrated to proportion as 1 g/mL (drug materials/solution), alcohol was added to the solution to 60% alcohol. Conclusions The optimized preparation process was found to be stable with a good reproducibility.
ABSTRACT
Objective To optimize the conditions for the extraction of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules,and for the preparation of their ?-CD inclusion compounds.Methods With the percentages of extracted volatile oil of Fructus tsaoko and Radix saposhnikoviae as the target marker,single-element experiments were conducted to determine the optimum conditions for extracting the volatile oil.The application rate of included volatile oil was used as the target marker to optimize the conditions for including the oils with ?-CD.Results The optimized extraction conditions were: with 8-fold water added,the sliced herbal medicine of Fructus tsaoko and Radix saposhnikoviae should be extracted for six hours without advanced soaking with water.The optimized inclusion conditions were: applying the saturated aqueous solution method,15-fold 85 % ethanol was used to dissolve the volatile oil,the proportion of the above solution and ?-CD being 1︰8;at 25 ℃,the volatile oil was included for 30 minutes with ultrasonic wave.Conclusion The established process can be used for the extraction and the inclusion of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules.