ABSTRACT
Objective: To investigate the immunosuppressive components in Leigongteng Duogan Tablet (LDT). Methods: High performance liquid chromatography (HPLC) was applied to establish the fingerprint for LDT from seven manufactures. The immunosuppressive effect of LDT was observed on Con A-induced mice spleen cells, and the cell proliferation and contents of IFN-γ were recorded. Then the partial least squares regression (PLSR) analysis was used to investigate its spectrum-effect relationship. Results: The HPLC fingerprint for LDT from seven manufactures was established, and 18 characteristic peaks were confirmed. The in vitro cell experimental results showed that the proliferation of mice spleen cells and secretion of IFN-γ were inhibited by all LDT significantly compared with the control group. According to the results of PLSR, No. 1, 2, 6, 7, 17, and 18 peaks were strongly related to the proliferation inhibition of mice spleen cells and No. 1, 2, 6, 7, 8, 9, 12, and 13 peaks were related to the secretion of IFN-γ. Conclusion: The No. 1, 2, 6, 7, 8, 9, 12, 13, 17, and 18 peaks are the principal immunosuppressive substances in LDT.