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1.
Gac. méd. boliv ; 35(2): 55-58, dic. 2012. tab
Article in Spanish | LILACS | ID: lil-737866

ABSTRACT

Objetivos: evaluar la respuesta a la prueba de la Intradermorreacción de Montenegro (IDRM) en áreas endémicas para leishmaniasis y enfermedad de Chagas. Métodos: se aplicó la IDRM a jóvenes sin antecedentes previos de leishmaniasis, en dos regiones endémicas para leishmaniasis y enfermedad de Chagas respectivamente. La prueba se aplicó en tres oportunidades con intervalo de dos meses entre aplicaciones, se evaluó la respuesta a la misma, por medición de la induración post inoculación. Resultados: No se encontró reacciones positivas de IDRM en ninguna de las dos regiones. La presencia de anticuerpos anti T. cruzi entre algunos de los participantes tampoco produjo reacciones positivas de la prueba. La inoculación de la IDRM no generó reacciones positivas al final de las tres aplicaciones. Conclusiones: por nuestros hallazgos nosotros concluimos que la IDRM podría ser utilizada como herramienta epidemiológica para leishmaniasis en Bolivia en situaciones en las que coexisten la leishmaniasis y el Chagas.


Objectives: to evaluate the response to intradermal test of Montenegro in endemic areas of leishmaniasis and Chagas disease. Methods: the intradermal test of Montenegro was applied to young people without evidences of previous leishmaniasis in two regions endemics to leishmaniasis or Chagas disease respectively. The test was applied in three times, each one after two months. The response to it was evaluated, by measuring of induration post inoculation. Results: it was found no positive reactions from intradermal test of Montenegro in both regions. The presence of antibodies anti-T cruzi in some participants not caused positive reactions of the test. The inoculation of intradermal test of Montenegro did not generate any positive reactions at end of the three inoculations. Conclusions: for our findings, we conclude that this test could be used as an epidemiological tool for leishmaniasis in Bolivian context where coexisting leishmaniasis and Chagas disease.


Subject(s)
Leishmaniasis, Cutaneous
2.
Bol. malariol. salud ambient ; 49(1): 107-116, jul. 2009. tab
Article in Spanish | LILACS | ID: lil-630399

ABSTRACT

Leishmania infantum es el agente etiológico de la leishmaniasis visceral (LV). Sin embargo, tanto en el Viejo Mundo como en el Nuevo Mundo(América Central), este protozoario ha sido involucrado en casos de leishmaniasis cutánea atípica (LCA). Evidencias clínicas, parasitológicas, moleculares y entomológicas han demostrado que una situación similar está ocurriendo en un área periurbana de Altagracia de Orituco (Guárico, Venezuela). Con la finalidad de contribuir al entendimiento de este nuevo escenario epidemiológico, se realizó un estudio transversal en el cual se determinó la reactividad a la prueba de leishmanina [Leishmanin skin test (LST)] y la seroprevalencia a antígenos crudos de Leishmania spp. y al antígeno específico rK39 mediante la prueba de ELISA en una muestra de ocho casos de LCA detectados en el periodo 1997-2000, en sus cohabitantes (n=15) y en una muestra de 233 de la comunidad igualmente expuestas a riesgo. La dermoprevalencia resultó ser de 31,6% (67/212). El 28,8% (67/233) resultó positivo a la prueba de ELISA usando promastigotas de L. infantum (=L. chagasi) y 13,3% (31/233) poseían anticuerpos anti-L. braziliensis. Así mismo, la prueba de ELISA usando el antígeno rK39 resultó positiva en uno de los ocho casos estudiados y en dos de sus cohabitantes; así como también en 13,7% (32/233) de los individuos residentes en la comunidad. Los resultados obtenidos indican la circulación de especies del subgénero Leishmania y del subgénero Viannia en el área en estudio y que la presencia de anticuerpos anti-Leishmania en general se encontró asociada a la edad y al tiempo de residencia en la zona


Leishmania infantum is the etiological agent of the visceral leishmaniasis (VL). However, in the OldWorld as well in the New World (Central America), thisprotozo an has also been involved in cases of atypicalcutaneous leishmaniasis (ACL). Clinical, molecularand entomological evidences have demonstrated thata similar situation is happening in a peri-urban areaof Altagracia de Orituco (Guárico state, Venezuela). With the purpose of contributing to the understandingof such an epidemiological scenario, a cross-sectionalstudy was carryed out using the leishmanin skin test(LST) and screening for the seroprevalence to crudeantigens of Leishmania spp. and rK39 by means ofthe ELISA test in cases of ACL reported in the period1997-2000 (n=8), among their co-inhabitants (n=15) and in a randomly selected sample of 233 people. Thedermoprevalence was 31.6% (67/212); 28.8% (67/233)were positive to the ELISA test using promastigotes of L. infantum (= L. chagasi) and 13.3% (31/233) hadantibodies anti-L. braziliensis. In addition, the test of ELISA using the antigen rK39 was positive in oneout of the eight cases studied and in two out of theirco-inhabitants; as well as in 13.7% (32/233) of theinhabitants of the community. The results obtained inthis epidemiological study indicate the circulation of species of the sub-genus Leishmania as well as of thesubgenus Viannia. The presence of antibodies anti-Leishmania in general was associated to the age andthe time of residence in the zone


Subject(s)
Humans , Male , Adolescent , Adult , Female , Child , Middle Aged , Enzyme-Linked Immunosorbent Assay , Leishmania infantum/pathogenicity , Leishmaniasis, Visceral/diagnosis , Eukaryota , Parasitology
3.
Chinese Journal of Infectious Diseases ; (12)1999.
Article in Chinese | WPRIM | ID: wpr-551834

ABSTRACT

Objective To clone amastin coding genes from different strains of Leishmania parasites. Methods Using amastin cDNA sequence as the reference, dbEST data base established by National Center Biotechnology Information (NCBI), USA, was searched by BLAST tool. A 309 bp DNA fragment of Leishmania major was found and used as the probe for the screening of a DNA library. The amastin gene of Leishmania major Abdou was cloned and sequenced. Specific primers were designed and amastin genes for Leishmania mexicana WR972, Leishmania brizeliensis and Leishmania amazonensis joseph were amplified by polymerase chain reaction. Results The amastin genes from 4 strains of Leishmania parasites were cloned and sequenced. It was found that all 4 amastin genes contained unique open reading frame of 552 bp and encoded amastin protein of 183 amino acid residues. Conclusion The amastin genes of 4 strains of Leishmania parasites were successfully cloned.

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