ABSTRACT
Objective: To develop a bioassay method to quantify the antiplatelet aggregation bioactivity (AAB) of crude Chuanxiong Rhizoma, decotion pieces, and its Chinese patent medicine for quality assessment. Methods: Chuanxiong Rhizoma sample was extracted in water by reflux. The level of AAB in extract was quantified in vitro. The blood was taken from the heart of rabbit. The platelet aggregating in platelet-rich plasma was induced by adenosine-5'-diphosphate disodium salt. The ratio of platelet inhibition was chosen as AAB marker. Sodium ferulate was a reference. The amount of AAB in aqueous extract was quantified by the Amount reaction of parallel line analysis (2.2) method. The AAB data of herbal sample was calculated by combining the AAB of extract with its extraction rate. Moreover, AAB amounts were quantified in the eight samples including crude Chuanxiong Rhizoma, decoction pieces, and Chinese patent medicines to verify this developed method. Results: Both sodium ferulate and Chuanxiong extract showed significant AAB (P < 0.01). The reliability test for quantifying AAB in solidum ferulate and Chuanxiong Rhizoma extract was passed through, and the measured value was valid. The correlation coefficient was 0.993 4 (n = 4) between the amounts of solidum ferulate in the concentration range of 15-60 mg/mL and their ratios of platelet inhibition. The RSD for the amounts of AAB was 3.43% (n = 6) by six replicated tests with the confidence limit rate of 23.90% (n = 6). The AAB amounts were significantly different among tested samples, i.e. 3.183, 2.068, 1.957, and 1.931 U/g for four Chuanxiong Rhizoma crude samples, 1.304 and 1.021 U/g for Chuanxiong Rhizoma decotion pieces and processed slice with yellow wine, 0.506 and 0.919 U/g for Suxiao Jiuxin Pills and Lemai Granule. Conclusion: The developed method can accurately quantify the level of AAB in Chuanxiong Rhizoma crude, decoction pieces, and Chinese patent medicines, which can be used to assess the product quality of Chuanxiong Rhizoma.
ABSTRACT
OBJECTIVE:To observe the efficacy of lemai granule in adjunctive therapy of vertebro-basilar artery insufficiency.METHODS:186 patients with vertebro-basilar artery insufficiency were randomly divided into 2 groups,lemai granule treatment group and control group.The control group were administered with flunarizine hydrochloride capsules and xueshuan xinmaining capsules,while the treatment group underwent adjunctive therapy with lemai granules besides the same treatment as the control group,the course of medication was 4 weeks in both groups.RESULTS:The total effective rates in treatment and control group were 95.8%and 80.2%(P
ABSTRACT
OBJECTIVE:To consummate the quality control criteria of Lemai granule.METHODS:Qualitation identifica?tion of Radix paeoniae rubra and nutgrass galingale rhizome in Lemai granule were performed by TLC,and the content of tanshinol-the chief component in Radix Salviae miltiorrhizae was determined by HPLC.RESULTS:The results showed that the color spectrum identification of Radix paeoniae rubra and nutgrass galingale rhizome in Lemai granule were positive.Good linear relationship was achieved between tanshinol sodium and peak area when the concentration range of which was0.203?g～2.030?g(r=0.9998).The average recovery rate was98.93%(RSD=1.04%).CONCLUSION:The established method in this research can be used as the quality control for Lemai granule.