Study on quantitative detection of bacterial endotoxin in recombinant novel coronavirus vaccine (CHO cell) by micro-dynamic chromogenic / 中国药理学通报

Aim To explore the feasibility of the micro- dynamic chromogenic method for quantitative detection of bacterial endotoxin in recombinant novel coronavirus vaccine ( CHO cell).Methods The micro-dynamic color method of Limulus reagent was used to establish a bacterial endotoxin standard curve.The dilution factor was determined through interference pre -experiment, the recoverv rate of the endotoxin added to the test so- J lution was determined, and the interference test to complete the quantitative detection test of the bacterial endotoxin content in the test product was performed, and the results were compared with those of the gel-clot method.Results Hie linear range of the concentration of the standard curve was 0.02 to 2.0 EU • mL 1 , and the regression equation of the standard curve was lgT =-0.302 7 lgC +2.858 7( r = 0.998 9).When recombinant novel coronavirus vaccine ( CHO cell) was cliluted 40 times or below, the micro -dynamic chromogenic reagent did not interfere with the bacterial endotoxin agglutination reaction, and the recovery rate was 50% to 200%.The test results were consistent with the gel- clot method.Conclusions The micro-dynamic chromogenic method can be used for the quantitative detection of bacterial endotoxins in recombinant novel coronavirus vaccine ( CHO cell) with accurate results, high sensitivity, and process monitoring.

A propósito de lastro, peso e leveza na instituição psicanalítica / About ballast, weight, and lightness in the psychoanalytic institution / A propósito del lastre, peso y levedad en la institución psicoanalítica / À propos de lest, poids et légèreté dans l'institution psychanalytique

Tomando o mito de Perseu como ponto de referência, o autor faz um breve voo sobre a instituição psicanalítica em geral, e sobre a instituição psicanalítica paulista, ressaltando seus momentos e atos fundadores. Deles, derivariam todo o lastro e o peso institucional, sendo dever de cada um a discriminação crítica entre um e outro, para que a psicanálise possa manter seu vigor original.

Taking the myth of Perseus as the reference point for his paper, the author writes a brief overview on the psychoanalytic institution in general. When the author writes about the psychoanalytic institution of São Paulo, he emphasizes its moments and founding acts, from which all the institutional "ballast" and weight would derive. The author believes everybody must critically distinguish that ballast from that weight in order to enable Psychoanalysis to keep its original vigor.

Tomando el mito de Perseo como punto de referencia, el autor realiza un breve vuelo sobre la institución psicoanalítica en general y sobre la institución psicoanalítica paulista, resaltando sus momentos y actos fundantes. De los mismos derivarían todo el lastre y el peso institucional, siendo deber de cada uno discriminar críticamente entre ambos para que el psicoanálisis pueda mantener su vigor original.

Tout en prenant comme point de repère le mythe de Persée, l'auteur fait un bref survol sur l'institution psychanalytique en général, et sur l'institution psychanalytique de São Paulo en particulier, en mettant en évidence leurs moments fondateurs et leurs actions fondatrices. C'étaient de ceux-ci qui dériveraient tout le lest et le poids institutionnel, et c'était le devoir de chacun faire la discrimination critique entre l'un et l'autre, pour que la psychanalyse puisse maintenir son vigueur original.

Language Evaluation Scale Trivandrum (LEST 3-6 years) – Development and Validation.

Language Evaluation Scale Trivandrum (LEST:3-6 years) with 31-items, was validated against ‘extended REELS’ with a community sample-606 children (3-6yrs). One item and two item delay as ‘LEST delay’ showed a sensitivity of (81%, 47%); specificity (68%, 94%), PPV (12%, 31%); NPV (98%, 97%) and accuracy (68.5%, 92%), respectively. LEST (3-6years) is a simple, valid, community screening tool.

Development and Validation of Language Evaluation Scale Trivandrum for Children Aged 0-3 years - LEST (0-3).

Objective: To develop and validate a simple screening tool which can be used in the Community to identify delay in language development among children of 0-3 years of age. Methods: The normal range for the 33-items of “Language Evaluation Scale Trivandrum for 0-3years-LEST(0-3)” were carefully selected from various existing language development charts and scales, by experts keeping in mind the face validity and content validity. The criterion validity was assessed using a community sample of 643 children of 0 to 3 years of age, including 340 (52.9%) boys. LEST (0-3) was validated against Receptive Expressive Energent Language Scale, for screening delay in language development among children of 0-3 years. Results: When one item delay was taken as ‘LEST delay’ (test positive), the sensitivity and specificity of LEST(0-3), was found to be 95.85% and 77.5%, respectively with a negative predictive value of 99.8% and LR (negative) of 0.05.When two item delay was taken as ‘LEST delay’ (test positive), the sensitivity and specificity of LEST(0-3), was found to be 66.7% and 94.8% respectively with a negative predictive value of 98.7% and LR (negative) of 0.35. The test-retest and inter-rater reliability were good and acceptable (Inter-class correlation of 0.69 for testretest and 0.94 for inter-rater). Conclusion: LEST (0-3) is a simple, reliable and valid screening tool for use in the community to identify children between 0-3 years with delay in language development, enabling early intervention practices.

Deux psychologies sociales marxistes dans l’Allemagne de l’époque du Mur de Berlin – Partie I: à l’Est / Two Marxist social psychologies in the Germany of the Berlin Wall epoch - Part I: the East

Cet article veut rendre compte d’une approche théorique qui a été élaborée dans la République Démocratique de l’Allemagne à l’époque du Mur de Berlin. Deux livres sont d’une grande importance pour leur présentation des principes théoriques qui y étaient empruntés au matérialisme dialectique ainsi que pour les rapports sur les recherches empiriques qui se sontinspirées de cette nouvelle construction théorique. La première édition du livre Einführung in die Marxistische Sozialpsychologie (Introduction à la Psychologie Sociale Marxiste) était publiée en 1966 par Hans Hiebsch et Martin Vorwerg. Les mêmes auteurs sont les éditeurs en 1979 d’un volume collectif Sozialpsychologie auquel ont collaboré onze de leurs collègues. Le présent article ne présente pas seulement les fondations théoriques de nature marxiste adoptées par lesauteurs de ces deux livres, il relate aussi leurs prises de position par rapport à la compatibilité ou incompatibilité de leurs nouvelles approches avec celles de traditions de recherche bien établiesen Amérique du Nord ou Europe Occidentale.

The aim of this article is to present the outlines of a theoretical approach that was initiated in the German Democratic Republic at the epoch of the Berlin Wall. Two books are especially important for their presentation of the theoretical principles that were borrowed frommaterial dialecticism and also for their reports on the empirical research that was effectuated in the new theoretical framework. The first edition of the book Einführung in die MarxistischeSozialpsychologie (Introducing Marxist Social Psychology) was published in 1966 and authored by Hans Hiebsch and Martin Vorwerg. The same authors edited in 1979 a collective book Sozialpsychologie in which participated also eleven more colleagues. The present article presents also the assessment by the authors about the compatibility or incompatibility of their new research approaches with well established social psychological approaches in North America andWestern Europe.