The effect of adenovirus mediated LIF and OSM co-expressing on proliferation and differentiation of umbilical cord blood CD34+ hematopoietic stem/progenitor cell / 中华微生物学和免疫学杂志

Objective To establish Ad-LIF-OSM transgenic feeder cells for the expansion of CD34+ hematopoietic stem/progenitor cell and tentatively study its effect in expansion and differentiation of cord blood hematopoietic stem cell(HSC) in vitro.Methods In the foundation of pAdTrack-CMV-LIF-polyA+promoterΔ,the OSM gene was inserted to the vector plasmid.Then we structure the transfer plasmid pAdTrack-CMV-LIF-polyA+promoterΔ-OSM.The transfer vector and backbone vector were further cotransfected for homologous recombination. The result pAdEasy-1-pAdTrack-CMV-LIF-polyA + promoterΔ-OSM homologous recombination plasmid were transfected into the human embryonic kidney 293 (QBI-293A) cells,leading to formation of the recombinant adenviruses Ad-LIF-OSM which co-expressing LIF and OSM.Infect the feeder layer cells with groups of Adenovirus,detection the expressing of LIF and OSM in WI-38 cells by RT-PCR and ELISA.Compares the stem cells differentiation and proliferation of the different experimental groups in vitro by transwell and cell counting.Results The sequencing results show that the OSM genes were anastomotic in Ad-LIF-OSM.LIF and OSM gene could be detected in feeder layer cells which infected by Ad-LIF-OSM.Exogenetic LIF and OSM have special effect in culturing HSC in vitro.Conclusion The adenoviral vector co-expressing LIF and OSM (Ad-LIF-OSM) were successfully constructed.Ad-LIF-OSM transgenic feeder cells can effectively proliferate umbilical cord blood CD34+ HSPC in vitro and delay it differentiate.

Effect of Erzhi Tiangui Granule on Endometrial Receptivity of Infertility Induced by Luteal Phase Deficiency / 中医杂志

Objective To study the effect and mechanism of Erzhi Tiangui Granule(Granule for promoting the uterine functions) on infertility induced by luteal phase deficiency.Methods Sixty infertility patients owing to luteal phase deficiency were randomized into the tested group(treated with Erzhi Tiangui Granule) and the control group(treated with Liuwei Dihuang Granule),30 in each.The improvement of TCM symptoms,the serum levels of estradiol(E2) and progestone(P),the expression of leukemia inhibiting factor(LIF) of luteal metaphase before and after treatment,and the pregnancy rate were observed.Results After treatment,the tested group was significantly better than the control group in improving the TCM symptoms(P0.05);the pregnancy rate in the tested group was higher than that in the control group(P